Experimental and Therapeutic Medicine, Год журнала: 2024, Номер 29(2)
Опубликована: Ноя. 25, 2024
In recent years, the Japanese population has been aging and risk of contracting various age‑related diseases increased. Thus, there is a need to analyze components that are characteristic examine their association with detect at an early stage. present study, microRNAs (miRNAs/miRs) in serum extracellular vesicles (EVs) 82‑102‑week‑old mice were analyzed identify miRNAs aging. Increased expression mmu‑miR‑21a‑5p was observed. These may be derived from senescent vascular endothelial cells, RNA‑sequencing data (GSE130727) HUVECs induced senesce by 4 Gy radiation revealed involved cell cycle DNA repair. Annotations senescence‑related pathways also identified. Reduced miR‑21‑5p target gene, which identical sequence humans mice, confirmed. age under similar conditions, increased senescence‑associated β‑galactosidase activity intracellular A portion secreted extracellularly internalizing tetraspanin‑positive EVs, into space. The study demonstrated upregulated secretion enhanced during senescence. findings suggested represents biomarker for
Язык: Английский