Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus

Gynecology Obstetrics and Reproductive Medicine, Год журнала: 2024, Номер unknown, С. 1 - 9

Опубликована: Апрель 8, 2024

OBJECTIVE: This study investigated vimentin expression in placentas of patients with preeclampsia and gestational diabetes mellitus (GDM). STUDY DESIGN: Placentas preeclamptic women (n=25), GDM control cases (n=25) were enrolled this study. Placental samples fixed zinc-formalin further processed for paraffin wax tissue embedding. Demographic laboratory parameters recorded. Vimentin immune activity was analyzed the placental sections immunohistochemistry. Sections imaged under a light microscope. A semiquantitative measurement done between groups by comparing signal significance calculated. Network construction pathway enrichment analysis conducted using Cytoscape (v3.10.1) ShinyGO, respectively. RESULTS: high group. The group showed positive cytotrophoblast syncytiotrophoblast cells connective villi generally recorded as negative villi, fibrinoid substances, Bioinformatic that AGE-RAGE signaling cancer-related pathways mainly observed Vimentin-associated pathways, which finally activate inflammatory both GDM. CONCLUSION: patterns reveal nuanced regulatory mechanisms, emphasizing need exploration into functional roles physiology pathology.

Язык: Английский

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A Systematic Review of Proteomics in Obesity: Unpacking the Molecular Puzzle

Current Obesity Reports, Год журнала: 2024, Номер 13(3), С. 403 - 438

Опубликована: Май 4, 2024

The present study aims to review the existing literature identify pathophysiological proteins in obesity by conducting a systematic of proteomics studies. Proteomics may reveal mechanisms development and clarify links between related diseases, improving our comprehension its clinical implications.

Язык: Английский

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The Role of RKIP in the Regulation of EMT in the Tumor Microenvironment

Cancers, Год журнала: 2022, Номер 14(19), С. 4596 - 4596

Опубликована: Сен. 22, 2022

The Raf Kinase Inhibitor Protein (RKIP) is a unique gene product that directly inhibits the Raf/Mek/Erk and NF-kB pathways in cancer cells resulting inhibition of cell proliferation, viability, EMT, metastasis. Additionally, RKIP involved regulation resistance to both chemotherapy immunotherapy. low expression many types responsible, part, for pathogenesis its multiple properties. EMT metastasis by led classification as tumor suppressor. However, mechanism which mediates inhibitory effects on metastases was not clear. We have proposed one involves negative various products mediate mesenchymal phenotype well positive epithelial via signaling cross talks between each product. examined several such Snail, vimentin, N-cadherin, laminin EPCAM E-cadherin laminin. found indeed these correlations were detected cross-talks. In addition, we also bioinformatic data sets different human cancers findings corroborated, large observed cross-talks with few exceptions some types. overall support underlying suppressor regulates Hence, development agent can selectively induce expressions should result activation pleiotropic anti-cancer activities including sensitization resistant respond chemotherapeutics immunotherapeutics.

Язык: Английский

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Virus-host Interactions in Early Japanese Encephalitis Virus Infection

Virus Research, Год журнала: 2023, Номер 331, С. 199120 - 199120

Опубликована: Май 1, 2023

Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic that can cause severe viral encephalitis. Initial interactions between JEV and host cells are required for productive infection initiation of the life cycle. The elucidation these critical, not only to understand pathogenesis infection, but also design efficient antiviral strategies. In this review, we outline known cellular components involved in entry into cells, with particular focus on initial virus-host cell interaction surface downstream early events such as endocytosis, membrane fusion, genome release.

Язык: Английский

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Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures

Journal of Biological Chemistry, Год журнала: 2023, Номер 299(8), С. 104963 - 104963

Опубликована: Июнь 24, 2023

Vimentin intermediate filaments form part of the cytoskeleton mesenchymal cells, but under pathological conditions often associated with inflammation, vimentin depolymerize as result phosphorylation or citrullination, and oligomers are secreted released into extracellular environment. In space, can bind surfaces cells matrix, interaction between trigger changes in cellular functions, such activation fibroblasts to a fibrotic phenotype. The mechanism by which binds external cell membranes whether alone act an adhesive anchor for is largely uncharacterized. Here, we show that various types (normal null fibroblasts, stem A549 lung carcinoma cells) attach spread on polyacrylamide hydrogel substrates covalently linked vimentin. Using traction force microscopy spheroid expansion assays, characterize how different respond Cell attachment spreading vimentin-coated inhibited hyaluronic acid degrading enzymes, synthase inhibitors, soluble heparin N-acetyl glucosamine, all treatments have little no effect same binding collagen-coated hydrogels. These studies highlight effectiveness substrate-bound ligand suggest carbohydrate structures, including glycocalyx glycosylated surface proteins contain novel class adhesion receptors either directly support substrate fine-tune properties.

Язык: Английский

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Enhancement of Wound Healing Efficacy by Chitosan-based Hydrocolloid on Sprague Dawley Rats

In Vivo, Год журнала: 2023, Номер 37(3), С. 1052 - 1064

Опубликована: Янв. 1, 2023

Background/Aim: Chitosan-based functional materials have attracted considerable attention worldwide for applications in wound healing, especially skin due to their efficiency hemostasis, anti-bacterial, and regeneration. Various chitosan-based products been developed healing applications, but most of these face limitations either efficacy or cost-effectiveness. Therefore, there is a need develop unique material that can handle all concerns be utilized acute chronic wounds. This study investigated mechanisms new hydrocolloid patches inflammatory reduction formation by using wound-induced Sprague Dawley Rats. Materials Methods: Our combined patch with chitosan achieve practical accessible medical would enhance healing. chitosan-embedded has shown significant influence preventing expansion inflammation increment on rat models. Results: The significantly increased the rate accelerated stage suppressing pro-inflammatory cytokines activity (e.g., TNF-α, IL-6, MCP-1, IL-1β). Moreover, product was effective promoting regeneration, demonstrated increase number fibroblasts through specific biomarkers vimentin, α-SMA, Ki-67, collagen I, TGF-β1). Conclusion: not only elucidated reducing enhancing proliferation, also provided cost-effective method dressing.

Язык: Английский

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Single-cell tracking as a tool for studying EMT-phenotypes

Experimental Cell Research, Год журнала: 2024, Номер 437(1), С. 113993 - 113993

Опубликована: Март 13, 2024

This article demonstrates that label-free single-cell video tracking is a useful approach for in vitro studies of Epithelial-Mesenchymal Transition (EMT). EMT highly heterogeneous process, involved wound healing, embryogenesis and cancer. The process promotes metastasis, increased understanding can aid development novel therapeutic strategies. role EMT-associated biomarkers depends on biological context, making it challenging to compare interpret data from different studies. We demonstrate comprehensive phenotype analysis. In this study we performed 72-h long recordings. quantified several behaviours at level during induced MDA-MB-468 cells. revealed notable variations migration speed, with dose-response patterns varying distributions speed. By registering cell morphologies the recording, determined preferred paths morphological transitions. also found clear association between speed morphology. elevated rates death, diminished proliferation, an increase mitotic failures followed by re-fusion sister-cells. method allows phenotypes lineages, which be particularly epigenetic Sister-cells were have significant similarities their speeds morphologies, illustrating heritability these traits.

Язык: Английский

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A review of protein-protein interaction and signaling pathway of Vimentin in cell regulation, morphology and cell differentiation in normal cells

Journal of Receptors and Signal Transduction, Год журнала: 2022, Номер 42(5), С. 512 - 520

Опубликована: Март 16, 2022

The Vimentin intermediate filament (VIF) is an essential cytoskeleton component. It shows dynamically changing expression patterns throughout various phases of the differentiation process, suggesting that protein physiologically important. Vimentin's functions have recently been clear, so Vimentin-deficient animals was described as a change morphology and signaling pathway. Recent research has discovered many vital roles for were previously unknown. VIF emerges organizer proteins involved in movement cell signaling. highly dynamic complicated phosphorylation seems to be regulator mechanism activities. Changes IF are often linked with cancer progression, especially those leading enhanced invasion cellular migration. This review will discuss function filaments normal physiology, adhesion structures, shape, pathways. genes interaction gene network discussed more studies. However, aimed at understanding different cascades interactions might offer novel methods creating therapeutic medicines. Enrichr GEO datasets used ontology (GO) pathway enrichment analyses. STRING online predict functional connections proteins-proteins, followed by Cytoscape analysis find master genes. revealed eight genes, Fas, Casp8, Casp6, Fadd, Ripk1, Des, Tnnc2, Tnnt3, required protein-protein differentiation.

Язык: Английский

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Relationship between Epithelial-to-Mesenchymal Transition and Tumor-Associated Macrophages in Colorectal Liver Metastases

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(24), С. 16197 - 16197

Опубликована: Дек. 19, 2022

The liver is the most common metastatic site in colorectal cancer (CRC) patients. Indeed, 25–30% of cases develop metastasis (CLM), showing an extremely poor 5-year survival rate and resistance to conventional anticancer therapies. Tumor-associated macrophages (TAMs) provide a nurturing microenvironment for CRC metastasis, promoting epithelial-to-mesenchymal transition (EMT) through TGF-β signaling pathway, thus driving tumor cells acquire mesenchymal properties that allow them migrate from primary invade new site. EMT known contribute disruption blood vessel integrity generation circulating (CTCs), being closely related high potential numerous solid cancers. Despite fact it well-recognized crosstalk between inflammatory crucial process, association role TAMs still poorly understood. In this review, we elaborated on exert induction during CLM development. Since are major source liver, also focused novel insights into their TGF-β-induced EMT.

Язык: Английский

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Bis-arylidene oxindoles for colorectal cancer nanotherapy

Bioorganic Chemistry, Год журнала: 2024, Номер 146, С. 107294 - 107294

Опубликована: Март 17, 2024

Язык: Английский

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