Serum angiotensin-converting enzyme 2 as a potential biomarker for SARS-CoV-2 infection and vaccine efficacy

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Окт. 12, 2022

Various species of the SARS-CoV-2 host cell receptor, angiotensin-converting enzyme 2 (ACE2), are present in serum, which may result from virus entry and subsequent proteolytic processing membrane receptor. We have recently demonstrated changes particular ACE2 infected humans, either cleaved fragments or circulating full-length species. Here, we further explore potential serum as a biomarker to test infection vaccine efficacy susceptible transgenic K18-hACE2 mice expressing human ACE2. First, samples derived challenged with lethal dose SARS-CoV-2, observed an increase levels fragment at day post-challenge, represent through entry. These elevated were maintained until death animals 6 post-challenge. The form displayed sizable peak 4, declined Noticeably, immunization two doses MVA-CoV2-S candidate prevented SARS-CoV-2. was extended vaccinated after re-challenge. findings highlight that could be for disease progression vaccination against

Язык: Английский

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Angiotensin converting enzyme 2 gene expression and markers of oxidative stress are correlated with disease severity in patients with COVID-19

Molecular Biology Reports, Год журнала: 2023, Номер 50(7), С. 5827 - 5836

Опубликована: Май 24, 2023

Oxidative stress is thought to play a significant role in the pathogenesis and severity of COVID-19. Additionally, angiotensin converting enzyme 2 (ACE2) expression may predict clinical course Accordingly, aim present study was evaluate association oxidative ACE2 with patients COVID-19.The comprised 40 COVID-19 matched healthy controls, recruited between September 2021 March 2022. ACE levels were measured using Hera plus SYBR Green qPCR kits GAPDH used as an internal control. Serum melatonin (MLT) levels, serum malondialdehyde (MDA) total antioxidant capacity (TAC) estimated ELISA. The correlations studied markers indicators disease evaluated. Significantly, lower observed compared controls. Patients had TAC MLT but higher MDA normal correlated diastolic blood pressure (DBP), Glasgow coma scale (GCS) scores, potassium levels. positively DBP, mean arterial (MAP), respiratory rate, GCS, platelet volume, creatinine significantly treated remdesivir inotropes. Receiver operating characteristic curve analysis demonstrates that all utility discriminating from controls.Increased increased poor outcomes hospitalized study. Melatonin supplementation provide adjuvant therapy decreasing death patients.

Язык: Английский

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GENOMIC PROFILING OF SARS-COV-2 STRAINS CIRCULATING IN SOUTH EASTERN REGION OF INDIA DURING THREE WAVES OF PANDEMIC

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 8, 2024

Continuous bio-surveillance of SARS-CoV-2 is an ongoing task at local, national and global levels since the pandemic onset for understanding genetic evolution vaccine efficacy. Present study was designed to track emergence new variants along duration three peaks infection in city Puducherry, India. A total 128 samples were subjected Illumina deep RNA sequencing. The results showed predominance uncommon, delta omicron first, second third waves respectively. most common pangolin lineage B.1.560 B.1.617.2. observed a 3133 11 mutations. Spike_D614G. set mutations key viral factors such as NS16 that are implicated be involved immune evasion. This may have impact on enhanced disease virulence, efficiency possible tolerance current antivirals. warrants further vitro studies understand significance While presented would also augment research evolutionary epidemiology SARS-CoV-2, it emphasizes need continuous monitoring predict forthcoming threats due or existing variants.

Язык: Английский

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Circulating angiotensin converting enzyme 2 and COVID-19

World Journal of Clinical Cases, Год журнала: 2022, Номер 10(34), С. 12470 - 12483

Опубликована: Дек. 1, 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has triggered a widespread outbreak since December 2019. SARS-CoV-2 infection-related illness been dubbed the disease 2019 (COVID-19) by World Health Organization. Asymptomatic and subclinical infections, hyper-inflammatory state, mortality are all examples of clinical signs. After attaching to angiotensin converting enzyme (ACE2) receptor, virus can enter cells through membrane fusion endocytosis. In addition enabling viruses cling target cells, connection between spike protein (S-protein) ACE2 may potentially impair functionality ACE2. Blood pressure is controlled ACE2, which catalyzes hydrolysis active vasoconstrictor octapeptide (Ang) II heptapeptide Ang-(1-7) free L-Phe. Additionally, Ang I be broken down into Ang-(1-9) metabolized Ang-(1-7). Numerous studies have demonstrated that circulating (cACE2) ability restore myocardial damage in variety cardiovascular diseases anti-inflammatory, antioxidant, anti-apoptotic, anti-cardiomyocyte fibrosis actions. There some suggestions for raising expression COVID-19 patients, might used as creation novel treatment therapies. With regard this, neutralized soluble recombinant human (hrsACE2), binds viral S-protein reduces organs, including heart, kidneys, lungs, lowering concentrations enhancing conversion This review aims investigate how presence cACE2 related. there will discussion number potential therapeutic approaches tip ACE/ACE-2 balance favor ACE-2/Ang-(1-7) axis.

Язык: Английский

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S protein, ACE2 and Host Cell Proteases in SARS-CoV2 Cell Entry and Infectivity; Is Soluble ACE2 a Two Blade Sword?

Опубликована: Дек. 2, 2022

Since the spread of deadly virus SARS-CoV2 in late 2019, researchers have restlessly been seeking for unraveling how factually enters host cells. Some proteins on each side interaction between and cells are involved as major contributors to this process: 1- nano-machine Spike protein behalf virus, 2- angiotensin converting enzyme II, mono-carboxypeptidase key component renin system cell, 3- some proteases exploited by SARS-CoV2, In review, complex process entrance into with contribution well sequential conformational changes tending increase probability complexification latter receptor cells, discussed. Besides, release catalytic ectodomain II its soluble form extracellular space positive or negative impact infectivity considered.

Язык: Английский

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