Astrocyte dysfunction alters GABAergic communication and ammonia metabolism in the streptozotocin-induced sporadic Alzheimer's disease model DOI Creative Commons

Éverton Carlos Brezolin,

Vitor Gayger-Dias, Vanessa-Fernanda Da Silva

и другие.

Journal of Alzheimer s Disease Reports, Год журнала: 2024, Номер 8(1), С. 1381 - 1393

Опубликована: Март 1, 2024

Background In the sporadic model of Alzheimer's disease (AD), induced by intracerebroventricular streptozotocin (STZ) administration, cognitive impairment is accompanied specific astrocytic changes in hippocampus prior to amyloid deposition. Objective Hypothesizing that synthesis GABA, via MAO-B, contributes ammonia elevation, thereby compromising antioxidant defense and ATP synthesis, possibly contributing damage, we determined hippocampal levels glutamine synthetase (GS), monoamine oxidase B (MAO-B) other enzymes related GABA metabolism. Methods Immunoblotting RT-PCR assays were carried out samples Wistar rats, at 4 16 weeks post-STZ, STZ-induced AD model, corresponding pre-amyloid phases, respectively. Results We observed a reduction GS activity increased MAO-B content, both weeks, reinforcing idea astroglial dysfunction precedes phase. These alterations an increase content ornithine decarboxylase 1 (ODC1), which catalyzes putrescine (substrate for MAO-B), gene expression arginine-glycine amidinotransferase (AGAT), enzyme involved creatine, generation agonists. only seen phase model. Conclusions Our findings contribute explain greater damage occurs energy metabolism this stage, addition GABAergic loss. The reinforce importance STZ further our understanding phases.

Язык: Английский

Glioprotective Effects of Sulforaphane in Hypothalamus: Focus on Aging Brain DOI
Camila Leite Santos, Fernanda Becker Weber, Adriane Belló‐Klein

и другие.

Neurochemical Research, Год журнала: 2024, Номер 49(9), С. 2505 - 2518

Опубликована: Июнь 17, 2024

Язык: Английский

Процитировано

1

Diverse Applications of the Anti-Diabetic Drug Metformin in Treating Human Disease DOI Creative Commons
Chris-Tiann Roberts,

Nils Raabe,

Lara Wiegand

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 17(12), С. 1601 - 1601

Опубликована: Ноя. 27, 2024

Metformin is a commonly used drug for treating type 2 diabetes. an inexpensive with low/no side effects and well tolerated in human patients of different ages. Recent therapeutic strategies disease have considered the benefits repurposing. This includes use anti-diabetic metformin. Accordingly, anti-inflammatory, anti-cancer, anti-viral, neuroprotective, cardioprotective potentials metformin deemed it suitable candidate plethora diseases. As results from preclinical studies using cellular animal model systems appear promising, clinical trials context non-diabetes-related illnesses been started. Here, we aim to provide comprehensive overview potential models its suggested relationship epigenetics ailments epigenetic components.

Язык: Английский

Процитировано

1

Sexual Dimorphism and Hypothalamic Astrocytes: Focus on Glioprotection DOI Creative Commons
Natalie K. Thomaz, Larissa Daniele Bobermin, André Quincozes‐Santos

и другие.

Neuroglia, Год журнала: 2024, Номер 5(3), С. 274 - 288

Опубликована: Авг. 2, 2024

Sexual dimorphism refers to biological differences between males and females in the same species, including morphological, physiological, behavioral characteristics. Steroid hormones are associated with changes several brain regions, as well pathophysiology of aging, obesity, neuropsychiatric diseases. The hypothalamus controls physiological processes, metabolism, reproduction, circadian rhythm, body homeostasis. Refined communication neurons glial cells, particularly astrocytes, coordinates hypothalamic functions. Therefore, from previously published studies, this review aims highlight sex-related rodent since we believe that region is essential for understanding dimorphic patterns influenced by steroid sex hormones. Thus, concepts sexual dimorphism, hypothalamic-pituitary-gonadal axis, role hormonal influence on astrocyte functions, neuroglial communication, disorders glioprotective mechanisms hypothalamus.

Язык: Английский

Процитировано

0

Astrocyte dysfunction alters GABAergic communication and ammonia metabolism in the streptozotocin-induced sporadic Alzheimer's disease model DOI Creative Commons

Éverton Carlos Brezolin,

Vitor Gayger-Dias, Vanessa-Fernanda Da Silva

и другие.

Journal of Alzheimer s Disease Reports, Год журнала: 2024, Номер 8(1), С. 1381 - 1393

Опубликована: Март 1, 2024

Background In the sporadic model of Alzheimer's disease (AD), induced by intracerebroventricular streptozotocin (STZ) administration, cognitive impairment is accompanied specific astrocytic changes in hippocampus prior to amyloid deposition. Objective Hypothesizing that synthesis GABA, via MAO-B, contributes ammonia elevation, thereby compromising antioxidant defense and ATP synthesis, possibly contributing damage, we determined hippocampal levels glutamine synthetase (GS), monoamine oxidase B (MAO-B) other enzymes related GABA metabolism. Methods Immunoblotting RT-PCR assays were carried out samples Wistar rats, at 4 16 weeks post-STZ, STZ-induced AD model, corresponding pre-amyloid phases, respectively. Results We observed a reduction GS activity increased MAO-B content, both weeks, reinforcing idea astroglial dysfunction precedes phase. These alterations an increase content ornithine decarboxylase 1 (ODC1), which catalyzes putrescine (substrate for MAO-B), gene expression arginine-glycine amidinotransferase (AGAT), enzyme involved creatine, generation agonists. only seen phase model. Conclusions Our findings contribute explain greater damage occurs energy metabolism this stage, addition GABAergic loss. The reinforce importance STZ further our understanding phases.

Язык: Английский

Процитировано

0