Neutral lipids restrict the mobility of broken DNA molecules during comet assays
Biology of the Cell,
Год журнала:
2025,
Номер
117(1)
Опубликована: Янв. 1, 2025
Abstract
One
widespread
technique
to
assess
in
relative
terms
the
amount
of
broken
DNA
present
genome
individual
cells
consists
immobilizing
cell's
nucleus
under
an
agarose
pad
(called
nucleoid)
and
subjecting
whole
electrophoresis
force
molecules
out
it.
Since
migrating
create
a
tail
behind
nucleoid,
this
is
named
comet
assay.
While
performing
assays
regularly,
we
systematically
observed
circular
regions
devoid
within
nucleoid
region.
We
characterize
here
that
these
correspond
clusters
neutral
(apolar)
lipids,
since
they
could
be
labeled
with
lipid‐dying
molecules,
increased
when
were
fed
oleic
acid,
irresponsive
electrophoretic
field.
Of
relevance,
de‐lipidation
assays,
either
vivo,
or
vitro
using
acetone,
show
lipids
(NL)
limit
ability
migrate
into
tail.
From
technical
point
view,
permits
wider
range
for
detection
molecules.
Biologically,
put
forward
notion
NL
contact
may
locally
exert
regulatory
functions
nucleus.
Язык: Английский
Parameters for sensing lipid loosening at the inner nuclear membrane by amphipathic helices
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 15, 2024
Amphipathic
helices
(AHs)
detect
differences
in
bulk
membrane
properties,
but
how
AHs
the
nuclear
surrounding
genome
is
not
well
understood.
Here,
we
computationally
screened
for
candidate
a
curated
list
of
characterized
and
putative
human
inner
(INM)
proteins.
Cell
biological
Язык: Английский
Lipids, from molecular mechanisms to diseases
Biochimie,
Год журнала:
2024,
Номер
227, С. 1 - 2
Опубликована: Ноя. 12, 2024
Язык: Английский
Neutral lipids restrict the mobility of broken DNA molecules during comet assays
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 13, 2024
Abstract
One
widespread
technique
to
assess
in
relative
terms
the
amount
of
broken
DNA
present
genome
individual
cells
consists
immobilizing
cell’s
nucleus
under
an
agarose
pad
(called
nucleoid)
and
subject
whole
electrophoresis
force
molecules
out
it.
Since
migrating
create
a
tail
behind
nucleoid,
this
is
named
comet
assay.
While
performing
assays
regularly,
we
systematically
observed
circular
regions
devoid
within
nucleoid
region.
We
characterize
here
that
these
correspond
clusters
neutral
(apolar)
lipids,
since
they
could
be
labelled
with
lipid-dying
molecules,
increased
when
were
fed
oleic
acid,
irresponsive
electrophoretic
field.
Of
relevance,
de-lipidation
either
vivo
,
or
vitro
show
lipids
limit
ability
migrate
into
tail.
From
technical
point
view,
permits
wider
range
for
detection
molecules.
Biologically,
put
forward
notion
contact
may
locally
exert
regulatory
functions
nucleus.
Язык: Английский
Nuclear lipids in chromatin regulation: Biological roles, experimental approaches and existing challenges
Biology of the Cell,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 8, 2024
Abstract
Lipids
are
crucial
for
various
cellular
functions.
Besides
the
storage
of
energy
equivalents,
these
include
forming
membrane
bilayers
and
serving
as
signaling
molecules.
While
significant
progress
has
been
made
in
comprehension
molecular
biology
lipids,
their
functions
cell
nucleus
remain
poorly
understood.
The
main
role
eukaryotic
is
to
provide
an
environment
regulation
chromatin
which
a
complex
DNA,
histones,
associated
proteins.
Recent
studies
suggest
that
nuclear
lipids
play
epigenetics.
Here,
we
discuss
experimental
methods
lipid‐chromatin
research,
including
biophysical,
structural,
approaches,
pointing
out
strengths
weaknesses.
We
take
view
have
far
more
widespread
impact
on
than
currently
acknowledged.
This
gap
mostly
due
existing
challenges
study
biology.
Several
new,
interdisciplinary
approaches
discussed
could
aid
elucidating
roles
gene
expression.
Язык: Английский