Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Окт. 20, 2023

Soybean root rot (SRR), caused by Fusarium oxysporum, is a severe soil-borne disease in soybean production worldwide, which adversely impacts the yield and quality of soybean. The most effective method for managing crop diseases decreasing reliance on chemical fungicides, such as Bacillus spp., via microbial biocontrol agents.In this study, soil-isolated strain BVE7 was identified B. velezensis, exhibiting broad-spectrum activity against various pathogens causing rot. sterile filtrate, at concentration 10%, demonstrated significant antifungal inhibiting conidial germination, production, mycelial growth F. oxysporum 61.11%, 73.44%, 85.42%, respectively, hyphal malformations. compound produced adaptability to standard environment. pot experiment showed that suspension could effectively control rot, with highest efficiency 75.13%. Furthermore, it considerably enhanced catalase, phenylalanine ammonia lyase, superoxide dismutase, peroxidase roots, while also preventing an increase malondialdehyde activity. By improving host resistance towards pathogens, damage fungi severity have been reduced.This study presents innovative utilization isolated from roots cold conditions, controlling oxysporum. findings highlight remarkable regional adaptive characteristics strain, making excellent candidate combating diverse environments. In conclusion, velezensis potential reducing SRR incidence can be considered viable option management.

Язык: Английский

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Unlocking plant defenses: Harnessing the power of beneficial microorganisms for induced systemic resistance in vegetables – A systematic review

Biological Control, Год журнала: 2023, Номер 188, С. 105428 - 105428

Опубликована: Дек. 16, 2023

Plants are susceptible to pathogen attacks, which employ strategies that overcome plant defenses and result in damage losses. The use of beneficial microorganisms can induce systemic resistance, leading greater efficiency disease control. objective this study was identify the main resistance genes metabolites involved process. A bibliographic search conducted on Scopus Web Science platforms, metadata were evaluated using Bibliometrix package R software. Subsequently, an investigation carried out their interactions with gene expression defense systems. In studies induced most cited Trichoderma, Bacillus, Pseudomonas, used Solanum lycopersicon L. followed by Arabidopsis thaliana. Among pathogens, employed Botrytis cinerea, Pseudomonas syringae, Rhizoctonia solani, Fusarium oxysporum. Beneficial influence responsible for signaling jasmonic acid, salicylic ethylene, production enzymes complex reactive oxygen species as mediators protection.

Язык: Английский

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Genomic and metabolomic insights into the antimicrobial compounds and plant growth-promoting potential of Bacillus velezensis B115

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 28, 2025

The B115 strain, isolated from the inter-root soil of healthy plants in a continuous cropping site Panax notoginseng, was identified as Bacillus velezensis by 16S rDNA sequence comparison and comparative genomic analysis. is strain beneficial microorganisms present zone plants, with favorable plant growth-promoting properties antagonistic effects against pathogen Fusarium oxysporum. However, whole genome remains unclear, thus restricting its potential applications. To address this gap, has been sequenced annotated to elucidate molecular mechanisms underlying antimicrobial activities. analysis revealed that comprises single circular chromosome 4,200,774 bp plasmid region 16,878 long, possessing GC content 45.95%. Moreover, 4349 protein-coding genes were predicted. Notably, contains substantial number (103) involved biosynthesis, transport, catabolism secondary metabolites. Through mining, 13 BGCs 540 encoding metabolites predicted roles identified, including members surfactin fengycin families. Utilizing LC–MS/MS technologies, 2318 detected fermentation broth B. B115, encompassing compounds such Corynebactin, Gamabufotalin, Pracinostat, Indoleacetic acid, (8)-Gingerol, Luteolin, Liquiritigenin, other antimicrobial, growth-promoting, antioxidant, antitumor properties. By exploring metabolite-related predicting based on whole-genome results, we further basis for ability promote growth inhibit activity.

Язык: Английский

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Eco-Friendly Biomass Production and Identification of Active Compounds of Paenibacillus polymyxa EB.KN35 with Potent Anti-Fusarium oxysporum Effect

Microorganisms, Год журнала: 2025, Номер 13(4), С. 800 - 800

Опубликована: Март 31, 2025

Fusarium oxysporum is a fungal plant pathogen for over 100 agricultural crop species. There are strategies managing wilt, including antagonistic bacteria that offer promising and sustainable effect. In this work, among the various endophytic bacterial strains, Paenibacillus polymyxa EB.KN35 was selected as best antifungal strain against F. oxysporum. For eco-friendly biomass production of bacterium, some byproducts were tested cultivation, soybean processing byproduct (SPBP) found to be suitable C/N source P. fermentation. The utilization 14 L bioreactor system fermentation achieved high productivity (3.46 × 1011 CFU/mL) in short time (8 h). bioactive compound analysis, secreting several growth-promoting compounds such GA3, IAA, kinetin, zeatin (via HPLC) eleven volatile GC–MS). docking study indicated (1, 2, 4, 9) may play significant role inhibiting results highlight potential reusing bioproduction with use biocontrol agent biofertilizer.

Язык: Английский

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Regional fingerprint, enrichment and flavor contribution of Bacillus community in Daqu for Chinese Baijiu

Food Research International, Год журнала: 2025, Номер 211, С. 116460 - 116460

Опубликована: Апрель 19, 2025

Язык: Английский

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A comparative metabolomics study of Panax ginseng infected by Fusarium oxysporum

Journal of Plant Pathology, Год журнала: 2025, Номер unknown

Опубликована: Май 8, 2025

Язык: Английский

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Isolation and identification of antagonistic fungi for biocontrol of Impatiens hawkeri leaf spot disease and their growth-promoting potential

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Май 16, 2025

Impatiens hawkeri , a plant of great horticultural significance with high ornamental and economic value, is frequently afflicted by leaf spot disease caused Stagonosporopsis cucurbitacearum effective biological control methods for this are yet to be fully explored. To address issue, the plate confrontation method was used isolate five antagonistic fungal strains from Quercus spinosa which showed strong inhibitory effects against S. inhibition rates 53.12% CG6, 43.42% CG7, 68.13% CY12, 54.30% CJ18, 67.23% CJ19, respectively. Among them, strain CY12 demonstrated highest antifungal activity, significantly suppressing mycelial growth . Through morphological observation sequence analyses ITS, TEF, RPB2, identified as Fusarium solani Further experiments revealed that could produce cell wall hydrolases exhibited multiple growth-promoting properties, such phosphorus potassium solubilization, nitrogen fixation, indoleacetic acid (IAA) production, it also produced various wall-degrading enzymes like amylase, cellulase, β -glucanase. Additionally, six common pathogens, an rate 71.41% pathogen causing Rhododendron disease. Pot indicated effectively reduced occurrence on I. plants, increased seed germination 93% in group 98.33%, promoted seedling growth. These results suggest endophytic fungus has providing potential resources development fertilizers, further exploration its field application action mechanisms necessary.

Язык: Английский

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Diversity of Antifungal Properties in Bacterial Isolates from Different Plant Species Growing Across Uzbekistan

Microorganisms, Год журнала: 2025, Номер 13(5), С. 1161 - 1161

Опубликована: Май 20, 2025

Plant-associated bacteria play a crucial role in protecting plants from pathogens, yet the diversity and antagonistic potential of these across different plant species remain underexplored, especially central Asia. To investigate competitive dynamics between phytopathogenic fungi plant-associated bacteria, we collected stem root samples 50 nine regions Uzbekistan. A total 3355 bacterial isolates were obtained (1896 roots 1459 shoots) screened for antifungal activity against six fungal resulting 432 isolates. These identified through 16S rDNA sequencing, revealing 65 three phyla: Firmicutes, Proteobacteria, Actinobacteria, predominantly respective families Bacillaceae, Pseudomonadaceae, Caryophanaceae. The Salsola vvedenskii hosted highest antagonists (26 species), while other harbored fewer. Plant showed strong associations with specific communities, 14 each hosting unique antagonists. Enzymatic profiling revealed functional diversity, Bacillus producing protease, cellulase, lipase activities, Pseudomonas excelled xylanase, glucanase, cellobiase production. B. mojavensis 9r-29 stood out by all enzymes. findings underscore ecological biocontrol natural ecosystems, offering promising candidates sustainable protection strategies.

Язык: Английский

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Evaluación del antagonismo de Bacillus spp. sobre hongos fitopatógenos de lima persa (Citrus x latifolia Tan.)

Revista Bio Ciencias, Год журнала: 2025, Номер unknown

Опубликована: Май 22, 2025

Lima persa (Citrus x latifolia Tan.) es un cultivo clave en Veracruz, con una producción afectada por enfermedades fúngicas. Para reducir el uso de fungicidas químicos, se ha investigado Agentes Control Biológico (ACB) como las bacterias del género Bacillus. En este estudio, evaluó efecto antagónico in vitro Bacillus velezensis (Bv) y dos cepas subtilis (Bs1 Bs2) sobre los patógenos causantes la muerte descendente (Lasiodiplodia sp.) antracnosis (Colletotrichum sp.), aislados huertas lima Tierra Blanca, Veracruz. Se midió Porcentaje Inhibición Crecimiento Radial (PICR) placas agar papa dextrosa. Las mostraron inhibición fitopatógenos evaluados. Bs2 presentó mayores porcentajes (66.69% 61.78%) frente a Lasiodiplodia sp. (Las1 Las2). Bs1 mostró 49.7% Colletotrichum (Colle). contraste, bajo antagónico, solo 8.4% Las1 nula otras cepas. Estos resultados indican que tienen alto potencial ACB contra afectan persa.

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Isolation and Identification of Burkholderia stagnalis YJ-2 from the Rhizosphere Soil of Woodsia ilvensis to Explore Its Potential as a Biocontrol Agent Against Plant Fungal Diseases

Microorganisms, Год журнала: 2025, Номер 13(6), С. 1289 - 1289

Опубликована: Май 31, 2025

Plant fungal diseases remain a major threat to global agricultural production, necessitating eco-friendly and sustainable strategies. Conventional chemical fungicides often lead the development of resistant pathogen strains cause environmental contamination. Therefore, biocontrol agents is particularly important. In this study, we identified Burkholderia stagnalis YJ-2 from rhizosphere soil Woodsia ilvensis as promising strain using 16S rRNA whole-genome sequencing. This demonstrated broad-spectrum antifungal activity against plant pathogens, with its bioactive extracts maintaining high stability across temperature range 25–100 °C pH 2–12. We used in vitro assays further show that metabolites B. disrupted hyphal morphology Valsa mali, resulting swelling, reduced branching, increased pigmentation. Fluorescence labeling confirmed stably colonized roots stems tomato wheat plants. Furthermore, various formulations microbial based on were evaluated for their efficacy pathogens. The seed-coating formulation notably protected seedlings Alternaria solani infection without affecting germination (p > 0.1), while wettable powder exhibited significant control effects early blight tomatoes, preventive treatment showing better than therapeutic treatment. Additionally, bone glue agent showed substantial inhibitory effect apple tree canker. Whole-genome analysis revealed 7,705,355 bp genome (67.68% GC content) 6858 coding genes 20 secondary metabolite clusters, including three clusters (YJ-2_GM002015-YJ-2_GM002048, YJ-2_GM0020090-YJ-2_GM002133, YJ-2_GM06534-YJ-2_GM006569) are related homologous biosynthetic gene known metabolites, such icosalide, ornibactin, sinapigladioside. knocked out core two metabolic found only YJ-2_GM006534-YJ-2_GM006569 cluster had corresponding function potential clusters. contrast wild-type YJ-2, deletion YJ-2_GM006563 by 8.79%. These findings highlight supporting theoretical foundation thereby promoting disease management.

Язык: Английский

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