Biotechnological uses of purified and characterized alkaline cellulase from extremophilic Bacillus pumilus VLC7 from Lake Van

World Journal of Microbiology and Biotechnology, Год журнала: 2025, Номер 41(2)

Опубликована: Фев. 1, 2025

Abstract The present study focuses on the isolation and characterization of a bacterium from Lake Van adapted to an alkaline pH environment with significant cellulase production potential. identified isolate, Bacillus pumilus VLC7 (GenBank Acc No: OR415888.1), exhibited highest activity among other alkaliphilic isolates. purification employing protein precipitation by ammonium sulfate, ultrafiltration, ion exchange chromatography resulted in 20-fold specific 16 U/mg protein. Cellulase had molecular weight 76 kDa, 3.13 mM K m 0.160 V max values. enzyme displayed maximum at 9.0 40 °C retained least 80% its temperatures 25–60 for 90 min 4–12 one hour. Stability tests also revealed enzyme’s resilience various reagents, metal ions, organic solvents, detergents. Furthermore, biotechnological applications were explored, demonstrating effectiveness fabric biopolishing (removing pilling), as well removal dyes. research findings underscore potential B. valuable source eco-friendly industrial biotechnology applications. Graphical

Язык: Английский

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Genome Mining and Antagonism of Stenotrophomonas geniculata MK‐1, Against Peanut Foliage Fungus Diseases

Journal of Basic Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Stenotrophomonas geniculata, a bacterium, has been recognized as an eco-friendly substitute for chemical fungicides in managing peanut foliar diseases, web blotch, and early leaf spot. Core genome pan-genome analysis identified that strain MK-1 belongs to nucleotide polymorphism (SNP) confirmed maltophilia. The research revealed S. geniculata had notable antagonistic impact on Peyronellaea arachidicola Cercospora demonstrated biocontrol efficacy of over 95% against spot blotch disease. nonredundant protein sequences (NR) database 4324 annotations related with 2682 genes similar MK-1. COG categorized 3041 into 22 functional groups, 33 distinct metabolic pathways associated 1851 Kyoto Encyclopedia Genes Genomes (KEGG) annotations. Most linked metabolism are found 380 carbohydrate 44 secondary metabolite biosynthesis. Carbohydrate-Active enZYmes (CAZy) 194 non-ribosomal synthesis metabolites. Pathogen-Host Interactions (PHI) showed reduced virulence MK-1, while unaffected pathogenicity counts were 52. can produce antifungal siderophores metabolites, peptide synthetase (NRPS), siderophores.

Язык: Английский

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Substitution of chemical fertilizer by biogas slurry maintain wheat yields by regulating soil properties and microbiomes

Environmental Technology & Innovation, Год журнала: 2025, Номер unknown, С. 104161 - 104161

Опубликована: Март 1, 2025

Язык: Английский

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Metagenomic Analysis of Wild Apple (Malus sieversii) Trees from Natural Habitats of Kazakhstan

Plants, Год журнала: 2025, Номер 14(10), С. 1511 - 1511

Опубликована: Май 18, 2025

Kazakhstan’s rich biodiversity includes diverse apple populations, notably the wild tree (Malus sieversii) prized for traits like disease resistance and adaptability, potentially aiding breeding programs. Analyzing their microbiomes offers insights into bacterial diversity how it influences development, making a reliable method understanding ecological interactions. In this research, 334 samples were collected from different mountain ranges in southeastern Kazakhstan. An analysis using nanopore-based 16S rRNA sequencing showed distinct similarity microbiome compositions of Zhongar Ile Alatau ranges, with predominance Pseudomonadaceae, Enterobacteriaceae, Microbacteriaceae. contrast, Ketmen ridge higher prevalence Enterobacteriaceae. Alongside less represented Pseudomonadaceae family, region, bacteria Xanthomonadaceae, Alcaligenaceae, Brucellaceae families also present. Across all regions, beneficial plant-associated identified, such as Pseudomonas veronii, Stenotrophomonas geniculata, Kocuria rhizophila, enhancing plant resilience. However, opportunistic phytopathogens detected, including viridiflava Serratia marcescens, particularly region. These findings highlight complex microbial interactions M. sieversii, thus offering key host—microbe relationships that can inform preservation efforts.

Язык: Английский

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Whole genome analysis of Stenotrophomonas geniculata MK2 and antagonism against Botrytis cinerea in strawberry

International Microbiology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

Язык: Английский

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