Опубликована: Янв. 1, 2024
Язык: Английский
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Янв. 9, 2025
The extracellular matrix (ECM) stores signaling molecules and facilitates mechanical biochemical in cells. However, the influence of biomimetic "rejuvenation" ECM structures on aging- degeneration-related cellular activities tissue repair is not well understood. We combined physical extrusion precise "on–off" alternating cross-linking methods to create anisotropic biomaterial microgels (MicroRod MicroSphere) explored how they regulate cell nucleus pulposus (NP) their potential antidegenerative effects intervertebral discs. NP cells exhibited aligned growth along surface MicroRod, enhanced proliferation, reduced apoptosis. This suggests an adaptive response involving adhesion mechanosensing, which causes cytoskeletal extension via environmental cues. maintain nuclear membrane integrity through YAP/TAZ pathway, activates cGAS-STING pathway rectify aging mechanisms. In vivo, MicroRod carries reduces inflammatory factor protease secretion degenerated discs, inhibiting degeneration promoting regeneration. Our findings highlight role stress maintaining activity antiaging harsh environments, providing a foundation for further research development biomaterials.
Язык: Английский
Процитировано
0
Small, Год журнала: 2025, Номер unknown
Опубликована: Фев. 21, 2025
Low back pain (LBP) resulting from sciatic nerve compression presents major challenges in management, as traditional therapies provide only short-term relief and pose risks of systemic toxicity. In this study, an innovative Fe3O4@ZIF-8-RVC (FZR) dual-responsive nanoplatform is introduced that integrates magnetic targeting with pH-sensitive, sustained drug release to overcome these limitations. The FZR encapsulates ropivacaine (RVC) within the ZIF-8-coated Fe3O4 core, enabling precise prolonged analgesia at injury site through guidance acid-triggered release. vitro vivo assessments indicate achieves high loading, acidic environments, excellent biocompatibility, significantly extending analgesic effects chronic models while minimizing exposure. Behavioral tests molecular analyses LBP rat confirm effectively suppresses pain-related neuronal activity central sensitization markers. This offers a safe, long-lasting, targeted therapeutic approach, holding strong potential for advancing related neuropathic conditions.
Язык: Английский
Процитировано
0
Journal of Controlled Release, Год журнала: 2025, Номер 381, С. 113556 - 113556
Опубликована: Фев. 28, 2025
Язык: Английский
Процитировано
0
Biomaterials, Год журнала: 2025, Номер unknown, С. 123321 - 123321
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
JOR Spine, Год журнала: 2024, Номер 7(4)
Опубликована: Ноя. 5, 2024
Abstract Background Extracellular vesicles (EVs) function as biomarkers and are crucial in cell communication regulation, with therapeutic potential for intervertebral disc (IVD)‐related low back pain (LBP). EV cargo is often affected by tissue health, which may affect the potential. There currently limited knowledge of how IVD cell‐derived EVs varies health differences proteomic profile predicted biological functions. Methods Our study purified from human conditioned media size‐exclusion chromatography. Nanoparticle tracking analysis was conducted to measure size concentration. Transmission electron microscopy Western blot were performed examine structure markers. Tandem mass tag‐mass spectrometry determine protein cargo. Results Most exosomes intermediate microvesicles an increasing amount linked disease progression. Of proteins detected, 88.6% shared across non‐degenerate, mildly‐degenerate, degenerate samples. GO KEGG analyses revealed that mildly‐degenerate samples most distinct, high abundance strongly associated extracellular matrix (ECM) organization structure. Shared proteins, highly expressed non‐degenerate samples, showed strong associations adhesion, ECM–receptor interaction, vesicle‐mediated transport, respectively. Conclusions findings indicate cells different degrees degeneration share a majority proteins. However, level expression differs grade. Cargo exhibits differences. A better understanding changes degenerative process provide novel information related molecular mechanisms underlying suggest new treatment modalities IVD‐related LBP.
Язык: Английский
Процитировано
1
Biomedical Technology, Год журнала: 2024, Номер 9, С. 100057 - 100057
Опубликована: Ноя. 21, 2024
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0