PROTACs in platelets: emerging antithrombotic strategies and future perspectives DOI
Justin S. Trory, Jordan Vautrinot, Carl May

и другие.

Current Opinion in Hematology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 21, 2024

Purpose of review Proteolysis-targeted chimeras (PROTACs) are heterobifunctional compounds that selectively target proteins for degradation and an emerging therapeutic modality to treat diseases such as cancer neurodegenerative disorders. This will widen the area application by highlighting ability PROTACs remove from anucleate platelets evaluate their antithrombotic potential. Recent findings Proteomic biochemical studies demonstrated human possess Ubiquitin Proteasomal System well E3 ligase cereblon (CRBN) therefore may be susceptible PROTAC-mediated protein degradation. confirmed CRBN ligand-based targeting generic tyrosine kinases, Btk and/or Fak lead efficacious selective in platelets. Downregulation Btk, a key player involved signalling thrombosis, but not haemostasis, resulted impaired in-vitro thrombus formation. Summary Platelets targeted have limited resynthesise proteins, ensuring long-term downregulation proteins. Therefore, serve additional research tool study platelet function offer new potential prevent thrombosis. Future should focus on enhancing cell specificity avoid on-target side effects other blood cells.

Язык: Английский

Targeted degradation of membrane and extracellular proteins with LYTACs DOI

Yuyang Li,

Yang Yang, Renshuai Zhang

и другие.

Acta Pharmacologica Sinica, Год журнала: 2024, Номер unknown

Опубликована: Авг. 5, 2024

Язык: Английский

Процитировано

6

Evolution of Alzheimer’s Disease Therapeutics: From Conventional Drugs to Medicinal Plants, Immunotherapy, Microbiotherapy and Nanotherapy DOI Creative Commons
Emma Ortiz, Pedro Montes, Citlali Ekaterina Rodríguez-Pérez

и другие.

Pharmaceutics, Год журнала: 2025, Номер 17(1), С. 128 - 128

Опубликована: Янв. 17, 2025

Alzheimer's disease (AD) represents an escalating global health crisis, constituting the leading cause of dementia among elderly and profoundly impairing their quality life. Current FDA-approved drugs, such as rivastigmine, donepezil, galantamine, memantine, offer only modest symptomatic relief are frequently associated with significant adverse effects. Faced this challenge in line advances understanding pathophysiology neurodegenerative condition, various innovative therapeutic strategies have been explored. Here, we review novel approaches inspired by advanced knowledge underlying pathophysiological mechanisms disease. Among alternatives, immunotherapy stands out, employing monoclonal antibodies to specifically target eliminate toxic proteins implicated AD. Additionally, use medicinal plants is examined, synergistic effects components may confer neuroprotective properties. The modulation gut microbiota also addressed a peripheral strategy that could influence neuroinflammatory degenerative processes brain. Furthermore, potential emerging approaches, microRNAs regulate key cellular nanotherapy, which enables precise drug delivery central nervous system, analyzed. Despite promising these strategies, incidence continues rise. Therefore, it proposed achieving effective treatment future require integration combined maximizing different interventions.

Язык: Английский

Процитировано

0

Insights From Protein Frustration Analysis of BRD4-Cereblon Degrader Ternary Complexes Show Separation of Strong from Weak Degraders DOI
Tianyi Yang, Elizaveta Mukhaleva, Wenyuan Wei

и другие.

RSC Medicinal Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Strong ligand directed degraders stabilize the hydrophobic residue burial between E3 ligase and target protein to be degraded. Weak destabilize ternary complex through multiple mechanisms.

Язык: Английский

Процитировано

0

Insights From Protein Frustration Analysis of BRD4-Cereblon Degrader Ternary Complexes Show Separation of Strong from Weak Degraders DOI Creative Commons
Tianyi Yang, Elizaveta Mukhaleva, Wenyuan Wei

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 10, 2025

Abstract PROteolysis TArgeting Chimeras (PROTACs), also known as Ligand-Directed Degraders (LDDs), are an innovative class of small molecules that leverage the ubiquitin-proteasome system to induce degradation target proteins. Structure based design methods not readily applicable for designing LDDs due dynamic nature ternary complexes. This study investigates properties five LDD-mediated BRD4-Cereblon complexes, focusing on challenges evaluating linker efficiency difficulty in identifying suitable computational metrics correlate well with cooperativity or propensity LDDs. We uncovered protein frustration, a concept originally developed understand folding, calculated residues protein-protein interface complexes recapitulate strength Our findings indicated hydrophobic among highly frustrated pairs, and they crucial distinguishing strong degraders from weak ones. By analyzing frustration patterns, we identified key interactions critical effectiveness complex. These insights provide practical guidelines prioritizing more efficient degraders, paving way development next-generation improved therapeutic potential.

Язык: Английский

Процитировано

0

Green procedures for synthesizing potential hNMDA receptor allosteric modulators through reduction and one-pot reductive acetylation of nitro(hetero)arenes using a superparamagnetic Fe3O4@APTMS@Cp2ZrClx (x = 0, 1, 2) nanocatalyst DOI Creative Commons
Hossein Mousavi, Behzad Zeynizadeh,

Farhad Sepehraddin

и другие.

Nanoscale Advances, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

In this research, we have developed diverse strategies for synthesizing potential h NMDA receptor allosteric modulators through reduction and one-pot reductive acetylation of nitro(hetero)arenes using a mesoporous zirconocene-containing nanocatalyst.

Язык: Английский

Процитировано

0

Preparation and characterization of ASDs improves the solubility and dissolution performance of a PROTAC drug DOI
Heng Zhang, Hengqian Wu, Lili Wang

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 106837 - 106837

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Integrating Proteolysis‐Targeting Chimeras (PROTACs) with Delivery Systems for More Efficient and Precise Targeted Protein Degradation DOI

Jiachan Lin,

Zirui Chen, Dan Zhang

и другие.

Macromolecular Rapid Communications, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Targeted protein degradation (TPD) using the proteolysis-targeting chimeras (PROTACs) is emerging as a revolutionary technology, offering potential strategy for cancer treatment by inducing of overexpressed oncogenic proteins in tumors. PROTACs function recruiting E3 ligases and utilizing ubiquitin-proteasome pathway (UPS) to catalyze target proteins. Compared traditional small molecules inhibitors, exhibit enhanced selectivity, ability overcome drug resistance, traditionally deemed "undruggable". However, poor water solubility low cellular permeability significantly limit their pharmacokinetic properties, while systemic toxicity may hinder clinical application. To address these limitations, strategies that integrate with delivery systems are gaining attention. This review summarizes latest advancements various enhance vivo efficacy reduce off-target effects PROTACs, including prototype nanoparticles, covalent modification-based prodrug strategies, innovative multi-headed designs, microneedle systems, discussing design principles associated challenges. The combination potent multifunctional holds promise accelerating translation improving therapeutic treatment.

Язык: Английский

Процитировано

0

PROTACs in platelets: emerging antithrombotic strategies and future perspectives DOI
Justin S. Trory, Jordan Vautrinot, Carl May

и другие.

Current Opinion in Hematology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 21, 2024

Purpose of review Proteolysis-targeted chimeras (PROTACs) are heterobifunctional compounds that selectively target proteins for degradation and an emerging therapeutic modality to treat diseases such as cancer neurodegenerative disorders. This will widen the area application by highlighting ability PROTACs remove from anucleate platelets evaluate their antithrombotic potential. Recent findings Proteomic biochemical studies demonstrated human possess Ubiquitin Proteasomal System well E3 ligase cereblon (CRBN) therefore may be susceptible PROTAC-mediated protein degradation. confirmed CRBN ligand-based targeting generic tyrosine kinases, Btk and/or Fak lead efficacious selective in platelets. Downregulation Btk, a key player involved signalling thrombosis, but not haemostasis, resulted impaired in-vitro thrombus formation. Summary Platelets targeted have limited resynthesise proteins, ensuring long-term downregulation proteins. Therefore, serve additional research tool study platelet function offer new potential prevent thrombosis. Future should focus on enhancing cell specificity avoid on-target side effects other blood cells.

Язык: Английский

Процитировано

0