Archives of Microbiology, Год журнала: 2024, Номер 207(1)
Опубликована: Дек. 30, 2024
Язык: Английский
Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160259 - 160259
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
International Journal of Antimicrobial Agents, Год журнала: 2025, Номер unknown, С. 107479 - 107479
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Theranostics, Год журнала: 2025, Номер 15(8), С. 3474 - 3489
Опубликована: Фев. 24, 2025
Background: Diabetic wounds infected with methicillin-resistant Staphylococcus aureus (MRSA) are challenging to heal due biofilm formation, which impairs conventional antibiotics limited penetration and severe side effects. Near-infrared (NIR)-driven nanomotors autonomous motion photothermal effects show promise for antibacterial therapy but often lack targeted specificity. Lysostaphin (Ly), an enzyme targeting bacterial cell walls, offers excellent potential against drug-resistant MRSA. Methods: A novel NIR-driven CSIL nanomotor has fabricated by co-loading indocyanine green (ICG) lysostaphin onto spinous yolk-shell structured C/SiO2@C nanoparticles. The motion, penetration, efficacy of evaluated in vitro, while their eradication wound healing performance assessed MRSA-infected diabetic mouse model using a cascade photodynamic (CPDT) strategy. Results: exhibit properties MRSA-targeting They can effectively eradicate MRSA biofilms both vitro vivo, suppress virulence biofilm-related genes, thus promoting shaping microenvironment dominated M2 macrophages. CPDT strategy is able avoid excessive ROS production thermal damage, enabling safe effective therapy. Conclusion: nanomotors, integrated photothermal, photodynamic, properties, represent novel, efficient approach wounds, offering significant advantages over antibiotics.
Язык: Английский
Процитировано
0
Frontiers in Microbiology, Год журнала: 2025, Номер 16
Опубликована: Март 20, 2025
Background Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant clinical challenge due to its multidrug resistance. Diacerein (DIA), primarily used treat degenerative joint diseases, has recently been found exhibit antibacterial activity, though specific mechanisms remain unclear. Methods The minimum inhibitory concentration (MIC) and bactericidal (MBC) of DIA, as well in - vitro combination susceptibility testing, were determined using the broth microdilution method. Additionally, resistance induction assays, time-growth curve measurements, membrane fluidity, intracellular protein levels, reactive oxygen species (ROS) assessed. inhibition clearance MRSA biofilms by DIA evaluated crystal violet staining method, with bacterial morphology observed via scanning electron microscopy confocal laser microscopy. Finally, transcriptome analysis was conducted identify gene expression changes treated RT-qPCR verification performed. Results MIC MBC against 32 μg/mL 128 μg/mL, respectively, synergistic effects when combined ampicillin. increased ROS levels fluidity MRSA, decreased soluble synthesis, altered morphology. significantly inhibited biofilm formation disrupted pre existing biofilms. Transcriptome revealed 1,045 differentially expressed genes between DIA-treated group control group, involving pathways such tricarboxylic acid cycle, phosphorylation, ribosome metabolism, nucleotide metabolism. Conclusion In summary, anti-biofilm activities does not easily induce Its may involve multiple aspects, including energy
Язык: Английский
Процитировано
0
Microbiological Research, Год журнала: 2024, Номер 292, С. 127995 - 127995
Опубликована: Дек. 7, 2024
Язык: Английский
Процитировано
2
Drug Design Development and Therapy, Год журнала: 2024, Номер Volume 18, С. 6413 - 6426
Опубликована: Дек. 1, 2024
In recent years, the incidence of fungal infections has been rising annually, especially among immunocompromised populations, posing a significant challenge to public health. Although antifungal medications provide some relief, escalating problem resistance sharply curtails their effectiveness, presenting an urgent clinical dilemma that demands immediate attention. Research shown is closely related quorum sensing (QS), and QS inhibitors (QSIs) are considered effective solution this issue. Garlic, as natural QSI, demonstrated effects in inhibiting growth, preventing biofilm formation, enhancing immunity, combating resistance. This study explores potential garlic mitigating drug identifies its key role mechanism, these findings offer new perspective for treatment infections, addressing increasingly severe However, application still faces several challenges, such ensuring standardization active ingredient extraction, well issues safety stability. Future research should focus on mechanism promote interdisciplinary collaboration develop more natural, effective, safe QSI drugs like garlic, while actively conducting trials validate efficacy safety. Additionally, incorporating advanced technologies nanotechnology enhance stability targeting, comprehensive strategy infections. Overall, Our provides scientific evidence supporting novel lays groundwork development future QSIs therapeutic use. It offers insights, particularly populations drug-resistant strains.
Язык: Английский
Процитировано
0
Archives of Microbiology, Год журнала: 2024, Номер 207(1)
Опубликована: Дек. 30, 2024
Язык: Английский
Процитировано
0