Research Square (Research Square),
Год журнала:
2022,
Номер
unknown
Опубликована: Ноя. 10, 2022
Abstract
Background
Small
cell
lung
cancer
(SCLC)
is
a
highly
lethal
malignant
tumor.
It
accounts
for
approximately
15%
of
newly
diagnosed
cancers.
Long
non-coding
RNAs
(lncRNAs)
can
regulate
gene
expression
and
contribute
to
tumorigenesis
through
interactions
with
microRNAs
(miRNAs).
However,
there
are
only
few
studies
reporting
the
profiles
lncRNAs,
miRNAs,
mRNAs
in
SCLC.
Also,
role
differentially
expressed
relation
competitive
endogenous
(ceRNA)
network
SCLC
remain
unclear.
Results
In
present
study,
we
first
performed
next
generation
sequencing
(NGS)
six
pairs
tumors
adjacent
non-cancerous
tissues
obtained
from
patients.
Overall,
29
48
510
were
found
be
samples
(|log
2
[fold
change]
|
>
1;
P
<
0.05).
Bioinformatics
analysis
was
predict
construct
lncRNA-miRNA-mRNA
ceRNA
network,
which
included
9
11
392
mRNAs.
Four
up-regulated
lncRNAs
related
regulatory
pathways
selected
validated
by
quantitative
PCR.
addition,
examined
most
upregulated
lncRNA,
TCONS_00020615,
cells.
We
that
TCONS_00020615
may
TCONS_00020615–hsa-miR-26b-5p–TPD52
pathway.
Conclusions
Taken
together,
our
findings
help
identify
some
potential
biomarkers
therapy
targets
study
provide
new
evidence
underlying
mechanism
Small
cell
lung
cancer
(SCLC)
is
a
highly
lethal
malignant
tumor.
It
accounts
for
approximately
15%
of
newly
diagnosed
cancers.
Long
non-coding
RNAs
(lncRNAs)
can
regulate
gene
expression
and
contribute
to
tumorigenesis
through
interactions
with
microRNAs
(miRNAs).
However,
there
are
only
few
studies
reporting
the
profiles
lncRNAs,
miRNAs,
mRNAs
in
SCLC.
Also,
role
differentially
expressed
relation
competitive
endogenous
(ceRNA)
network
SCLC
remain
unclear.In
present
study,
we
first
performed
next
generation
sequencing
(NGS)
six
pairs
tumors
adjacent
non-cancerous
tissues
obtained
from
patients.
Overall,
29
48
510
were
found
be
samples
(|log2[fold
change]
|>
1;
P
<
0.05).
Bioinformatics
analysis
was
predict
construct
lncRNA-miRNA-mRNA
ceRNA
network,
which
included
9
11
392
mRNAs.
Four
up-regulated
lncRNAs
related
regulatory
pathways
selected
validated
by
quantitative
PCR.
In
addition,
examined
most
upregulated
lncRNA,
TCONS_00020615,
cells.
We
that
TCONS_00020615
may
TCONS_00020615-hsa-miR-26b-5p-TPD52
pathway.Our
study
provided
comprehensive
tissues.
constructed
networks
provide
new
evidence
underlying
mechanism
also
lncRNA
carcinogenesis
American Journal of Cancer Research,
Год журнала:
2024,
Номер
14(3), С. 996 - 1014
Опубликована: Янв. 1, 2024
RNASEH1-AS1,
a
long
non-coding
RNA
(lncRNA)
divergently
transcribed
from
the
antisense
strand
of
its
neighboring
protein-coding
gene
ribonuclease
H1
(RNASEH1),
has
recently
been
demonstrated
to
be
involved
in
tumor
progression.
However,
association
between
RNASEH1-AS1
and
hepatocellular
carcinoma
(HCC)
remains
unclear.
In
present
study,
first,
expression
HCC
correlation
with
clinicopathological
features,
prognosis,
diagnosis,
immune
cell
infiltration
patients
was
inspected
using
relevant
R
packages
based
on
The
Cancer
Genome
Atlas
(TCGA)
data.
found
up-regulated
most
cancer
types,
including
HCC,
overexpression
significantly
associated
histologic
grade
AFP
level
as
well
poor
an
independent
risk
factor
affecting
overall
survival
good
diagnostic
prognostic
values
for
HCC.
inversely
plasmacytoid
dendritic
cells
(pDC),
B
neutrophils.
Second,
total
1109
positively
co-expressed
genes
(PCEGs)
were
screened
out
by
analysis
batches
(|Spearman's
r|
>0.4
adjusted
Disease Markers,
Год журнала:
2022,
Номер
2022, С. 1 - 12
Опубликована: Авг. 29, 2022
LncRNAs
are
known
to
regulate
a
plethora
of
key
events
cellular
processes;
however,
little
is
about
the
function
lncRNAs
in
autophagy.
Here
current
study,
we
report
lncRNA-IGFBP4
which
has
previously
been
proliferation
and
reprogramming
cancer
cells,
but
its
role
autophagy
not
yet
known.
We
found
that
serum
starvation
provokes
autophagy-induced
downregulation
levels.
Next,
determined
c-Myc
can
negatively
HeLa
cells.
Phenotypically,
upon
depletion
lncRNA-IGFBP4,
cells
undergo
through
ULK1/Beclin1
signaling.
Furthermore,
TCGA
data
analysis,
lncRNA-IGFB4
overexpressed
most
cancers
including
cervical
cancer.
Based
on
these
findings,
conclude
maintains
homeostasis
regulating
World Journal of Gastrointestinal Oncology,
Год журнала:
2023,
Номер
15(4), С. 644 - 664
Опубликована: Апрель 11, 2023
The
expression
of
brain
cytoplasmic
RNA1
(BCYRN1)
is
linked
to
the
clinicopathology
and
prognosis
several
types
cancers,
among
which
hepatocellular
carcinoma
(HCC)
one
most
frequent
cancer
worldwide.To
explore
prognostic
value
immunotherapeutic
potential
BCYRN1
in
HCC
by
bioinformatics
meta-analysis.Information
was
obtained
from
Cancer
Genome
Atlas
database.
First,
correlation
between
clinicopathologic
characteristics
patients
explored.
Univariate
multivariate
regression
analyses
were
employed
examine
relationship
prognosis.
Secondly,
functions
pathways
explored
means
enrichment
analysis
differentially-expressed
genes.
relationships
tumor
microenvironment,
immune
cell
infiltration,
checkpoint,
drug
sensitivity
immunotherapy
effect
also
investigated.
Finally,
three
major
databases
searched
used
conduct
a
meta-analysis
on
patient
prognosis.BCYRN1
significantly
higher
compared
normal
tissues
poor
clinicopathological
characteristics.
Enrichment
showed
that
regulates
extracellular
matrix
transmission
signaling
molecules,
participates
metabolism
nutrients,
such
as
proteins,
tumor-related
pathways.
efficacy
immunotherapy.
Furthermore,
this
study
overexpression
related
worse
outcome
patients.Overexpression
relates
may
be
factor
target
HCC.
