Emodin influence pyroptosis-related Caspase 1-GSDMD axis alleviated cerebral ischemia-reperfusion injury in rats DOI Creative Commons
Liang Tao, Guofang Zhang, Xiaolin Hu

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Abstract Background Cerebrovascular disease encompasses a wide range of conditions characterized by cerebrovascular lesions or disruptions in blood flow. Ischemic stroke, among these conditions, is the most prevalent and known for its substantial morbidity, disability, mortality rates, making it leading cause global disability. Effective management ischemia-reperfusion injury holds paramount importance stroke treatment, regardless whether thrombolytic therapy administered. Previous studies have shown that Emodin exhibits anti-inflammatory neuroprotective properties, providing protection against various organs modulating pyroptosis. However, precise molecular mechanisms underlying effects cerebral remain poorly understood. Therefore, objective this study was to elucidate context ischemic stroke. Methods SD rats were randomly assigned different groups, including control group, sham operation model intervention group with varying dosages. Cerebral induced using middle artery occlusion (MCAO) method. Intraperitoneal injections 10mg/kg, 20mg/kg 40 mg/kg administered assess neurological changes rats. The modified Neurological Severity Score (mNSS) used evaluate deficits. infarct volume ratio determined through TTC staining, while HE staining employed observe pathomorphological changes. Using Western blotting (WB) technique immunofluorescence, we investigated expression levels cellular localization proteins associated cell pyroptosis, NLRP3, Caspase 1 GSDMD. Additionally, enzyme-linked immunosorbent assay (ELISA) measure IL-1β IL-18. whole animal approved Affiliated Hospital Zunyi Medical University (approval number KLLY(A)-2021-083) all methods are reported accordance ARRIVE guidelines. Results significant beneficial improving deficits caused injury. It effectively reduces volume, alleviates cytopathic damage suppresses pyroptosis-related proteins, Furthermore, decreases pro-inflammatory cytokines IL-18, thus attenuating inflammatory response. Conclusions upregulated after demonstrates rats, potentially mediated 1-GSDMD axis.

Язык: Английский

Evaluation of Bioactivity of Essential Oils: Cytotoxic/Genotoxic Effects on Colorectal Cancer Cell Lines, Antibacterial Activity, and Survival of Lactic Acid Bacteria DOI Creative Commons
Katarína Kozics, Monika Mesárošová, Monika Šramková

и другие.

Molecules, Год журнала: 2025, Номер 30(4), С. 890 - 890

Опубликована: Фев. 14, 2025

Colorectal cancer (CRC) ranks among the most frequently diagnosed malignancies and is associated with a significantly high mortality rate. In recent years, increasing attention has been directed toward naturally derived substances anticancer properties. our study, we focused on determining biological antibacterial effects of selected essential oils (EOs)-peppermint, oregano, tea tree, lemon, lavender, frankincense, oil blends (Zengest OnGuard). Analyses were performed human colon carcinoma cell lines (HCT-116 HT-29). The cytotoxic (MTT assay), genotoxic (comet reactive oxygen species levels (ROS-Glo™ H2O2 Assay) EOs determined. found that all studied have potential cyto/genotoxic CRC after 24 h exposure. results revealed Zengest, frankincense showed statistically highest [IC50 0.05 µg/mL] compared to other oils. These induced DNA damage also increased ROS levels. On hand, peppermint was shown lowest effect 0.67 HT-29 line. We evaluated OnGuard blend, their impact viability beneficial bacteria models, including Lacticaseibacillus rhamnosus, Lactiplantibacillus plantarum, paracasei, Lactobacillus brevis, pentosus, Weizmannia coagulans. Oregano exhibited strong activity, an inhibition zone 31 mm, while tree zones ranging from 12 15 mm. (oregano, OnGuard) demonstrated effects, MICs 0.5 µg/mL. Peppermint, Zengest blend did not inhibit growth lactic acid or W. coagulans, thus bacterial survival. they effects.

Язык: Английский

Процитировано

0
Biological activities of hydroxyanthracene derivatives (HADs) from Aloe species and their potential uses DOI Creative Commons
Julio Enríquez Merino, Alexandra G. Durán, Nuria Chinchilla

и другие.

Phytochemistry Reviews, Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

Язык: Английский

Процитировано

0
Antioxidant and Anti-Inflammatory Activities of Methanol Extract of Senna septemtrionalis (Viv.) H.S. Irwin & Barneby Through Nrf2/HO-1-Mediated Inhibition of NF-κB Signaling in LPS-Stimulated Mouse Microglial Cells DOI Open Access

Jae Sung Lim,

Xiangying Li, Da Young Lee

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1932 - 1932

Опубликована: Фев. 24, 2025

Botanical extracts are recognized in traditional medicine for their therapeutic potential and safety standards. viable sustainable alternatives to synthetic drugs, being essential drug discovery various diseases. Senna septemtrionalis (Viv.) H.S. Irwin & Barneby is a medical plant traditionally used treat inflammation. However, its antioxidant anti-inflammatory properties the molecular pathways activated microglial cells require further investigation. Therefore, this study examines of methanol (SMEs) lipopolysaccharide (LPS)-stimulated mouse cells. SMEs significantly inhibit LPS-induced nitric oxide (NO) proinflammatory cytokine production, which mediated through dephosphorylation mitogen-activated protein kinases inhibition nuclear factor kappa B (NF-κB) translocation into nucleus. Additionally, SME treatment upregulated expression erythroid 2-related 2 (Nrf2) heme oxygenase (HO)-1, reducing oxidative stress, indicated by decrease reactive oxygen species restoration total glutathione content LPS-stimulated BV2 The inhibitory effects on inflammatory mediator production NF-κB were reversed Sn-protoporphyrin, specific HO-1 inhibitor. These findings demonstrate that protects activating Nrf2/HO-1 pathway inhibiting translocation.

Язык: Английский

Процитировано

0
Drug‐likeness evaluation and inhibitory mechanism of the emodin derivative on cardiac fibrosis based on metastasis‐associated protein 3 DOI Open Access
Heng Liu, Runze Li, Zhixia Wang

и другие.

British Journal of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

Abstract Background and Purpose Emodin inhibits cardiac fibrosis through metastasis‐associated protein 3 (MTA3), but its limited bioavailability hinders clinical application. To enhance emodin's potential, a new derivative, emodin succinyl ethyl ester, was synthesised by modifying the 3′‐OH position. This study assessed drug‐likeness, anti‐fibrotic properties molecular mechanisms involving MTA3. Experimental Approach Drug‐likeness of derivative were evaluated using computational‐aided drug design (CADD). Transverse aortic constriction (TAC)‐induced Angiotensin II (Ang II)‐stimulated fibroblasts used in vivo ex vivo, respectively, to determine effects on fibroblast transdifferentiation. Bioinformatics analysis, CADD, chromatin immunoprecipitation (ChIP), luciferase reporter assays functional experiments employed predict, identify validate relationship between MTA3 upstream transcription factors. Key Results The exhibited superior drug‐likeness compared effectively inhibiting transdifferentiation restored expression. E2F1 identified validated as transcriptional regulator, promoting α‐SMA COL1A2 expression, directly reducing expression fibroblasts. demonstrated stronger binding site than emodin, enhancing action. Conclusions Implications shows improved potent inhibition targeting E2F1, disrupting pro‐fibrotic function, restoring halting progression. advances derivative's potential therapy for provides insights into mechanisms.

Язык: Английский

Процитировано

0
Emodin influence pyroptosis-related Caspase 1-GSDMD axis alleviated cerebral ischemia-reperfusion injury in rats DOI Creative Commons
Liang Tao, Guofang Zhang, Xiaolin Hu

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Abstract Background Cerebrovascular disease encompasses a wide range of conditions characterized by cerebrovascular lesions or disruptions in blood flow. Ischemic stroke, among these conditions, is the most prevalent and known for its substantial morbidity, disability, mortality rates, making it leading cause global disability. Effective management ischemia-reperfusion injury holds paramount importance stroke treatment, regardless whether thrombolytic therapy administered. Previous studies have shown that Emodin exhibits anti-inflammatory neuroprotective properties, providing protection against various organs modulating pyroptosis. However, precise molecular mechanisms underlying effects cerebral remain poorly understood. Therefore, objective this study was to elucidate context ischemic stroke. Methods SD rats were randomly assigned different groups, including control group, sham operation model intervention group with varying dosages. Cerebral induced using middle artery occlusion (MCAO) method. Intraperitoneal injections 10mg/kg, 20mg/kg 40 mg/kg administered assess neurological changes rats. The modified Neurological Severity Score (mNSS) used evaluate deficits. infarct volume ratio determined through TTC staining, while HE staining employed observe pathomorphological changes. Using Western blotting (WB) technique immunofluorescence, we investigated expression levels cellular localization proteins associated cell pyroptosis, NLRP3, Caspase 1 GSDMD. Additionally, enzyme-linked immunosorbent assay (ELISA) measure IL-1β IL-18. whole animal approved Affiliated Hospital Zunyi Medical University (approval number KLLY(A)-2021-083) all methods are reported accordance ARRIVE guidelines. Results significant beneficial improving deficits caused injury. It effectively reduces volume, alleviates cytopathic damage suppresses pyroptosis-related proteins, Furthermore, decreases pro-inflammatory cytokines IL-18, thus attenuating inflammatory response. Conclusions upregulated after demonstrates rats, potentially mediated 1-GSDMD axis.

Язык: Английский

Процитировано

0