Genes and Environment,
Год журнала:
2024,
Номер
46(1)
Опубликована: Июнь 3, 2024
Abstract
Background
Rosmarinic
acid
(RA)
has
a
wide
range
of
beneficial
effects
on
human
health.
On
the
other
hand,
RA
been
reported
to
induce
metal-mediated
reactive
oxygen
species
(ROS)
generation
and
DNA
damage.
However,
its
mechanism
remains
unknown.
In
this
study,
clarify
underlying
mechanism,
we
analyzed
damage
in
isolated
treated
with
analog
isorinic
acid.
Results
plus
Cu(II),
but
not
Fe(III),
significantly
increased
8-oxo-7,8-dihydro-2’-deoxyguanosine
(8-oxodG)
formation,
an
indicator
oxidative
damage,
calf
thymus
DNA.
Furthermore,
comparison
8-oxodG
formation
induced
by
suggested
that
catechol
groups
could
be
associated
their
abilities
form
8-oxodG.
Interestingly,
Cu(II)
was
markedly
enhanced
addition
NADH,
endogenous
reductant.
To
elucidate
Cu(II)-induced
examined
32
P-labeled
presence
Cu(II).
caused
cleavage,
which
piperidine
treatment,
suggesting
causes
only
strand
breakage
also
base
modification.
inhibited
catalase
(H
2
O
scavenger),
bathocuproine
(Cu(I)
chelator),
methional
(scavenger
variety
ROS
than
•
OH)
typical
OH
scavengers
SOD,
indicating
involvement
H
,
Cu(I),
OH.
cleavage
site
analysis
showing
RA-induced
site-specific
(frequently
at
thymine
some
cytosine
residues)
supports
OH,
because
without
specificity.
Based
these
results,
Cu(I)
concomitant
autoxidation
lead
production
Cu(I)-hydroperoxide,
induces
o
-Quinone
-semiquinone
radicals
are
likely
again
reduced
dramatically
increases
particularly
low
concentrations
RA.
Conclusions
physiologically
relevant
effectively
through
redox
cycle
reactions
copper
NADH.
Cell Biochemistry and Function,
Год журнала:
2024,
Номер
42(7)
Опубликована: Окт. 1, 2024
ABSTRACT
Ocimum
species
have
a
great
interest
in
different
traditional
medicinal
systems.
This
study
examined
the
chemical
composition,
antioxidant
properties,
enzyme
inhibitory
effects,
and
antibacterial
antifungal
activities
of
aerial
parts
gratissimum
,
americanum
basilicum
from
Comoros
Islands.
The
extracts
were
analyzed
using
high‐performance
liquid
chromatography‐mass
spectrometry
(HPLC‐MS)
to
determine
their
composition.
Antioxidant
activity
was
assessed
2,2‐Diphenyl‐1‐picrylhydrazyl
(DPPH),
2,2′‐Azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic
acid)
diammonium
salt
(ABTS),
cupric
reducing
capacity
(CUPRAC),
ferric
power
(FRAP),
chelating
ability,
phosphomolybdenum
radical
scavenging
assays.
Enzyme
against
acetylcholinesterase
(AChE),
butrylcholinesterase
(BChE),
tyrosinase,
amylase,
glucosidase
evaluated
spectrophotometric
methods.
Antibacterial
tested
broth
microdilution
method
selected
pathogenic
microorganisms.
enzymes
proteins
silico
methods
with
biomolecules
these
plants.
In
addition,
111
metabolites
identified
advanced
HPLC/MS
techniques.
most
significant
number
detected
compounds
derivatives
hydroxycinnamic
acids,
followed
by
flavonoid
glycosides
aglycones
hydroxybenzoic
acids.
All
three
exhibited
activities,
O.
best‐reducing
abilities
CUPRAC
FRAP
assays
revealed
that
had
potent
effect
on
tyrosinase
(48.01
±
3.89
mg
kojic
acid
equivalent
[KAE]/g),
amylase
(1.08
0.02
mmol
acarbose
[ACAE]/g).
tests
demonstrated
possess
broad‐spectrum
activity.
Molecular
docking
results
showed
remarkable
binding
energies
target
proteins.
molecular
dynamics
simulations
chicoric
MurE
Staphylococcus
aureus
complex
as
promising
drug
candidate.
These
findings
support
medical
nutraceutical
uses
suggest
possibilities
for
natural
functional
applications.
Phytochemical Analysis,
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 16, 2024
Abstract
Introduction
Skin
cancer
poses
a
significant
health
risk
globally,
necessitating
effective
and
safe
therapeutic
interventions.
Epigallocatechin‐3‐gallate
(EGCG)
from
green
tea
rosmarinic
acid
(RA)
herbs
like
rosemary
offer
promising
anticancer
properties.
Combining
these
compounds
may
enhance
their
effectiveness,
prompting
the
need
for
reliable
analytical
method
to
quantify
them.
Objective
Herein,
we
present
development
validation
of
high‐performance
thin‐layer
chromatography
(HPTLC)
concurrent
quantification
EGCG
RA
in
lipid‐based
nanoparticles
biological
samples.
Methodology
The
underwent
optimisation
through
design
experiments
(DoE),
resulting
establishment
robust
chromatographic
conditions.
separation
process
utilised
aluminium
HPTLC
plates
coated
with
silica
gel
60
F
254
as
stationary
phase,
mobile
phase
comprising
ethyl
acetate,
toluene,
formic
acid,
methanol
ratio
4:4:1:1
v/v.
Results
retention
factor
(
R
f
)
values
obtained
were
0.38
0.61
RA.
demonstrated
linearity
over
range
100–500
ng/band
both
excellent
correlation
coefficients.
Limits
detection
determined,
indicating
high
sensitivity.
Precision
evaluations
revealed
relative
standard
deviation
below
2%,
ensuring
reproducibility.
Recovery
assays
nanoparticles,
plasma,
urine
samples
recoveries
(96.2%–102.1%).
Forced
degradation
studies
minimal
under
various
stress
conditions,
photolytic
showing
least
impact.
Conclusion
developed
offers
rapid,
sensitive,
approach
quantifying
RA,
laying
groundwork
further
investigation
agents
alone
combination
therapies.
Plants,
Год журнала:
2023,
Номер
13(1), С. 89 - 89
Опубликована: Дек. 27, 2023
Rosmarinus
officinalis
L.
compounds,
especially
its
main
polyphenolic
carnosic
acid
(CA)
and
rosmarinic
(RA),
influence
various
facets
of
cancer
biology,
making
them
valuable
assets
in
the
ongoing
fight
against
cancer.
These
two
secondary
metabolites
exhibit
formidable
antioxidant
properties
that
are
a
pivotal
contributor
development
Their
antitumor
effect
has
been
related
to
diverse
mechanisms.
In
case
CA,
it
capacity
induce
cell
death
cells
through
rise
ROS
levels
within
cells,
inhibition
protein
kinase
AKT,
activation
autophagy-related
genes
(ATG)
disrupt
mitochondrial
membrane
potential.
Regarding
RA,
actions
encompass
apoptosis
induction
caspase
activation,
proliferation
by
interrupting
cycle
progression
epigenetic
regulation,
antioxidative
stress-induced
DNA
damage,
interference
with
angiogenesis
curtail
tumor
growth.
To
understand
molecular
interaction
between
rosemary
compounds
(CA
RA)
is
involved
inflammation,
S100A8,
we
have
performed
series
docking
analyses
using
available
three-dimensional
structures
(PDBID:
1IRJ,
1MR8,
4GGF).
The
ligands
showed
different
binding
intensities
active
sites
target
molecules,
except
for
CA
1MR8
protein.
Rosmarinic
acid
(RosA)
is
a
natural
polyphenol
compound
that
has
been
shown
to
be
effective
in
the
treatment
of
inflammatory
disease
and
variety
malignant
tumors.
However,
its
specific
mechanism
for
lung
adenocarcinoma
(LUAD)
not
fully
elucidated.
Therefore,
this
study
aims
clarify
RosA
LUAD
by
integrating
bioinformatics,
network
pharmacology
vivo
experiments,
explore
potential
active
ingredients
traditional
Chinese
medicine
treating
LUAD.
Firstly,
was
used
screen
targets,
LUAD-related
differential
expressed
genes
(DEGs)
were
acquired
from
GEO
database.
The
intersection
regulated
(RDEGs)
obtained
through
Venn
diagram.
Secondly,
GO
KEGG
enrichment
analysis
RDEGs
performed,
protein–protein
interaction
networks
(PPIs)
constructed
identify
visualize
hub
RDEGs.
Then,
molecular
docking
between
further
evaluation
carried
out
using
bioinformatics
predictive
value
Finally,
verified
establishing
xenograft
model
NSCLC
nude
mouse.
Bioinformatics
other
showed
that,
compared
with
control
group,
expressions
MMP-1,
MMP-9,
IGFBP3
PLAU
tissues
significantly
up-regulated,
PPARG
FABP4
down-regulated,
these
had
In
experimental
results
could
inhibit
growth
transplanted
tumors
mice
bearing
cancer
cells,
reduce
positive
expression
Ki67
tumor
tissue,
hinder
proliferation
cells.
Upregulated
activating
PPAR
signaling
pathway
increases
level
ROS
promotes
apoptosis
addition,
can
also
MMP-9
IGFBP3,
migration
invasion
tissue
This
demonstrated
induce
regulating
proliferation,
thereby
exerting
anti-LUAD
effects.
provides
new
insight
into
therapeutic
avenue
