Isolation of Tumour‐Derived Extracellular Vesicles From the Plasma of Dogs Affected by Intracranial Tumours Showing Heterologous and Cross‐Species Tropism: A Pilot Study DOI Creative Commons
Simona Vincenti, Alessandro Villa,

Zemira De Mitri

и другие.

Veterinary and Comparative Oncology, Год журнала: 2024, Номер 22(4), С. 621 - 628

Опубликована: Сен. 23, 2024

Canine and human brain tumours exhibit similar incidence rates prognoses. Recent studies have demonstrated that extracellular vesicles derived from patients (PDEVs) can be loaded with contrast agents tumour tropism in murine models. We showed a previous study gadolinium-labelled EVs canine gliomas (cPDEVs) selectively targets glioblastoma cells animal As further step, we investigated the potential heterologous cross-species of cPDEVs tumours. With perspective imminent clinical application as both markers drug delivery tools, successfully established isolation protocol for confirmed aseptic conditions procedure therefore sterility isolated EVs. To assess functionality tool, they were indocyanine green (ICG) injected into models cancer vivo fluorescence biodistribution studies. Biodistribution analysis mice revealed ICG-loaded subcutaneous accumulated exclusively neoplastic tissue, even when evaluated 24 h post-injection, thus showing selective nanoparticles. these tests, safe isolating loading autologous various markers, thereby paving way testing phase. These significant findings suggest use theranostic tool management tumours, promising implications translational medicine applications future.

Язык: Английский

Harnessing the Potential of Exosomes in Therapeutic Interventions for Brain Disorders DOI Open Access
Bai Lu,

Leijie Yu,

Mao‐Sheng Ran

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2491 - 2491

Опубликована: Март 11, 2025

Exosomes, which are nano-sized natural vesicles secreted by cells, crucial for intercellular communication and interactions, playing a significant role in various physiological pathological processes. Their characteristics, such as low toxicity immunogenicity, high biocompatibility, remarkable drug delivery capabilities—particularly their capacity to traverse the blood–brain barrier—make exosomes highly promising vehicles administration treatment of brain disorders. This review provides comprehensive overview exosome biogenesis isolation techniques, strategies loading functionalization exosomes, exosome-mediated barrier penetration mechanisms, with particular emphasis on recent advances exosome-based Finally, we address opportunities challenges associated utilizing system brain, summarizing barriers clinical translation proposing future research directions.

Язык: Английский

Процитировано

1

Extracellular vesicles as therapeutic modulators of neuroinflammation in Alzheimer’s disease: a focus on signaling mechanisms DOI Creative Commons

Jianping Han,

Xue Zhang, Lixing Kang

и другие.

Journal of Neuroinflammation, Год журнала: 2025, Номер 22(1)

Опубликована: Апрель 25, 2025

Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by the accumulation of amyloid-beta (Aβ) plaques and tau tangles, which contribute significantly to neuroinflammation, central driver pathogenesis. The activation microglia astrocytes, coupled with complex interactions between Aβ pathologies innate immune response, leads cascade inflammatory events. This process triggers release pro-inflammatory cytokines chemokines, exacerbating neuronal damage fostering cycle chronic inflammation that accelerates neurodegeneration. Key signaling pathways, such as nuclear factor-kappa B (NF-κB), Janus kinase/signal transducer activator transcription (JAK/STAT), mitogen-activated protein kinase (MAPK), phosphoinositide 3-kinase/protein (PI3K/Akt), are involved in regulating production these mediators, offering potential therapeutic targets for AD. Recently, extracellular vesicles (EVs) have emerged promising tool AD therapy, due their ability cross blood-brain barrier (BBB) deliver agents. Despite challenges standardizing EV-based therapies ensuring safety, EVs offer novel approach modulating neuroinflammation promoting neuroregeneration. review aims highlight intricate relationship emerging role therapeutics advancing treatment strategies.

Язык: Английский

Процитировано

0

Role of Extracellular Vesicles in the Progression of Brain Tumors DOI Creative Commons
Gabriella Schiera, Carlo Maria Di Liegro,

Francesco Vento

и другие.

Biology, Год журнала: 2024, Номер 13(8), С. 586 - 586

Опубликована: Авг. 2, 2024

Brain tumors, and, in particular, glioblastoma (GBM), are among the most aggressive forms of cancer. In spite advancement available therapies, both diagnosis and treatments still unable to ensure pathology-free survival GBM patients for more than 12–15 months. At basis poor ability cope with brain we can consider: (i) intra-tumor heterogeneity; (ii) heterogeneity tumor properties when compare different patients; (iii) blood–brain barrier (BBB), which makes difficult isolation tumor-specific biomarkers delivering therapeutic drugs brain. Recently, it is becoming increasingly clear that cancer cells release large amounts extracellular vesicles (EVs) transport metabolites, proteins, classes RNAs, DNA, lipids. These structures involved pathological process characterize any particular form Moreover, EVs able cross BBB directions. Starting from these observations, researchers now evaluating possibility use purified organic fluids (first all, blood saliva), order obtain, through non-invasive methods (liquid biopsy), biomarkers, perhaps, also obtaining nanocarriers targeted drugs.

Язык: Английский

Процитировано

3

Extracellular vesicles as delivery vehicles and therapeutic agents for glioblastoma treatment: A systematic review of in vitro and in vivo preclinical studies DOI Creative Commons
J. C. Ng, Nabil Ajwad, Shing Cheng Tan

и другие.

Asian Journal of Pharmaceutical Sciences, Год журнала: 2025, Номер unknown, С. 101043 - 101043

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Extracellular Vesicle-Based Drug Delivery Systems in Cancer Therapy DOI Open Access

Jiahao Wu,

Z.X. Jin,

Tao Fu

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4835 - 4835

Опубликована: Май 19, 2025

Extracellular vesicles (EVs) are lipid bilayer-enclosed particles secreted by cells and ubiquitously present in various biofluids. They not only mediate intercellular communication but also serve as promising drug carriers that capable of delivering therapeutic agents to target through their inherent physicochemical properties. In this review, we summarized the recent advances EV isolation techniques innovative drug-loading strategies. Furthermore, emphasized distinct advantages applications EVs derived from different cellular sources cancer treatment. Finally, critically evaluated ongoing clinical trials utilizing for delivery systematically assessed both opportunities challenges associated with implementing EV-based systems therapy.

Язык: Английский

Процитировано

0

Isolation of Tumour‐Derived Extracellular Vesicles From the Plasma of Dogs Affected by Intracranial Tumours Showing Heterologous and Cross‐Species Tropism: A Pilot Study DOI Creative Commons
Simona Vincenti, Alessandro Villa,

Zemira De Mitri

и другие.

Veterinary and Comparative Oncology, Год журнала: 2024, Номер 22(4), С. 621 - 628

Опубликована: Сен. 23, 2024

Canine and human brain tumours exhibit similar incidence rates prognoses. Recent studies have demonstrated that extracellular vesicles derived from patients (PDEVs) can be loaded with contrast agents tumour tropism in murine models. We showed a previous study gadolinium-labelled EVs canine gliomas (cPDEVs) selectively targets glioblastoma cells animal As further step, we investigated the potential heterologous cross-species of cPDEVs tumours. With perspective imminent clinical application as both markers drug delivery tools, successfully established isolation protocol for confirmed aseptic conditions procedure therefore sterility isolated EVs. To assess functionality tool, they were indocyanine green (ICG) injected into models cancer vivo fluorescence biodistribution studies. Biodistribution analysis mice revealed ICG-loaded subcutaneous accumulated exclusively neoplastic tissue, even when evaluated 24 h post-injection, thus showing selective nanoparticles. these tests, safe isolating loading autologous various markers, thereby paving way testing phase. These significant findings suggest use theranostic tool management tumours, promising implications translational medicine applications future.

Язык: Английский

Процитировано

0