Effect of dietary Dendrobium nobile Lindl. On production performance, immune function and caecal microbiota of Chishui black-bone hens

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 21, 2025

The objective of this study was to investigate the effects dietary supplementation Dendrobium nobile Lindl. (DNL) on production performance, immune function, and caecal microbiota Chishui black-bone hens. A total one hundred eighty 600-day-old hens were randomly allocated two experimental groups, with six replicates 15 chickens per group. control group fed a basal diet, supplemented 2100 mg/kg DNL in diet. results demonstrated that compared group, addition diet significantly (P < 0.05) increased average egg rate from 38.97 44.95%. Interferon-gamma (IFN-γ) levels lower than whereas serum immunoglobulin (IgA) higher 0.05). However, adding exerted no significant effect quality. Asp, Ser, Gly, Leu, Phe, His, Pro, TAA, EAA, NEAA eggs those Additionally, EAA/TAA groups 40.38% 40.76%, respectively, EAA/NEAA 76.62% 74.72%, both which 40% 60% thresholds specified WHO/HAO ideal protein guidelines. also indicated had contents palmitic acid (C16:0), stearic (C18:0), (C16:1), oleic (C18:ln9c), behenolic (C22:0), (C16:), saturated fatty acids (SFA), monounsaturated (MUFA) In contrast, hexadecenoic (C15:0), heptadecanoic (C17:0), α-linolenic (C18:3n3), arachidonic (C20:0), C10 (C17:1), linoleic (C18:2n6c), eicosapentaenoic (C20:2), Docosapentaenoic Acid (DPA), polyunsaturated (PUFA), n-6PUFA reduced Moreover, when added hens, composition intestinal flora altered. Specifically, relative abundances Bacteroides, Lactobacillus, Subdoligranulum, Parabacteroides increased, Desulfovibrio, Lachnoclostridium, Ruminococcaceae, Fournierella, Faecalibacterium, Oribacterium decreased. conclusion, feed can increase IgA reduce IFN-γ content during late laying period. Furthermore, it enhance types amino eggs, as well ratio proteins. Simultaneously, improved microbial community abundance beneficial bacteria.

Язык: Английский

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Inulin alleviates chronic ketamine-induced impairments in memory and prepulse inhibition by regulating the gut microbiota, inflammation, and kynurenine pathway

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 294, С. 139503 - 139503

Опубликована: Янв. 5, 2025

Язык: Английский

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Ultrasound-assisted metabolite detection in different extraction processes of Bletilla striata and bitter metabolite detection

Ultrasonics Sonochemistry, Год журнала: 2025, Номер 114, С. 107266 - 107266

Опубликована: Фев. 10, 2025

Bletilla striata, a medicinal orchidaceous plant, is recognized for its significant pharmacological value. However, the lack of comparative metabolomic data across different extraction methods analyzing bioactive components has significantly undervalued application potential B. striata in traditional Chinese medicine market. Using six ultrasound-assisted and UPLC-MS/MS, this study identified 1,945 metabolites extracts. The dominant categories were lipids (51.35%), flavonoids (18.00%), phenolic acids (12.51%). KEGG analysis revealed alterations isoflavonoids biosynthesis pathways. Thirteen bitter metabolites, including cinnamic acid, tubers, underscoring their applications, such as anti-inflammatory, antioxidant antibacterial activities. Optimizing can better preserve extracts, thereby enhancing applications food pharmaceutical industries.

Язык: Английский

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Lactobacillus Johnsonii YH1136 alleviates schizophrenia-like behavior in mice: a gut–microbiota–brain axis hypothesis study

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 2, 2025

Based on the microbiota-gut-brain axis (MGBA) hypothesis, probiotics play an increasingly important role in treating various psychiatric disorders. Schizophrenia (SCZ) is a common mental disease with complex pathogenesis and challenging to treat. Although studies have elucidated mechanisms associated interactions between SCZ, few specifically used as therapeutic intervention for SCZ. Accordingly, current study determines whether L. johnsonii YH1136 effectively prevents SCZ-like behavior mice identifies key microbes metabolites. An SCZ mouse model was established by intraperitoneal injection of MK-801; administered via oral gavage. significantly improves abnormal behaviors, including psychomotor hyperactivity sociability alleviates aberrant enzyme expression tryptophan metabolism mice. Additionally, upregulates hippocampal brain-derived neurotrophic factor (BDNF) levels while downregulating 2,3-dioxygenase (TDO2), indoleamine-pyrrole 1 (IDO1), kynurenine aminotransferase (KAT1). Subsequent 16S rRNA sequencing intestinal contents suggests that modulates gut flora structure composition increasing relative abundance Lactobacillus decreasing Dubosiella N-acetylneuraminic acid hypoxanthine are serum metabolites mediating interaction MGBA These results partially reveal mechanism underlying effects mice, supporting development probiotic formulations against

Язык: Английский

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Asiaticoside improves depressive-like behavior in mice with chronic unpredictable mild stress through modulation of the gut microbiota

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Окт. 18, 2024

Background Asiaticoside, the main active ingredient of Centella asiatica, is a pentacyclic triterpenoid compound. Previous studies have suggested that asiaticoside possesses neuroprotective and anti-depressive properties, however, mechanism its anti-depressant action not fully understood. In recent years, growing body research on anti-depressants has focused microbiota-gut-brain axis, we noted disruption gut microbial community structure diversity can induce or exacerbate depression, which plays key role in regulation depression. Methods Behavioral experiments were conducted to detect depression-like behavior mice through sucrose preference, forced swimming, open field tests. Additionally, composition short-chain fatty acid (SCFA) levels mouse feces analyzed 16S rRNA sequencing gas chromatography-mass spectrometry (GC-MS). Hippocampal brain-derived neurotrophic factor (BDNF) 5-hydroxytryptamine receptor 1A (5-HT1A) expression was assessed by western blotting. Changes serum inflammatory factors, neurotransmitters, hormones measured using ELISA. Results This study revealed oral administration significantly improved chronic unpredictable mild stress (CUMS) mice. It partially restored CUMS mice, altered SCFA metabolism, regulated hypothalamic–pituitary–adrenal axis (HPA axis) levels, upregulated BDNF 5-HT1A protein expression, increased serotonin (5-hydroxytryptamine, 5-HT) concentration. These findings reveal exerts antidepressant effects via axis. Conclusions results model.

Язык: Английский

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Exerkine irisin mitigates cognitive impairment by suppressing gut-brain axis-mediated inflammation

Journal of Advanced Research, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

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Mendelian randomization analysis of causal and druggable circulating inflammatory proteins in schizophrenia

Frontiers in Psychiatry, Год журнала: 2024, Номер 15

Опубликована: Окт. 31, 2024

Schizophrenia (SZ) is a severe mental disorder with complex origins. Observational studies suggested that inflammatory factors may play role in the pathophysiology of SZ and we aim to investigate potential genetic connection between them by examining causal impact circulating proteins on SZ.

Язык: Английский

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Xin-Jia-Tong-Xie-Yao-Fang Restores the Intestinal Barrier to Alleviate Irritable Bowel Syndrome Via Microbial Butyrate Mediated Pi3k/Akt Pathway Suppression

Опубликована: Янв. 1, 2024

Язык: Английский

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