Microorganisms,
Год журнала:
2024,
Номер
12(5), С. 998 - 998
Опубликована: Май 15, 2024
Surfactin
is
widely
used
in
the
petroleum
extraction,
cosmetics,
biopharmaceuticals
and
agriculture
industries.
It
possesses
antibacterial
antiviral
activities
can
reduce
interfacial
tension.
Bacillus
are
commonly
as
production
chassis,
but
wild-type
subtilis
168
cannot
synthesise
surfactin.
In
this
study,
phosphopantetheinyl
transferase
(PPTase)
gene
sfp*
(with
a
T
base
removed)
was
overexpressed
enzyme
activity
restored,
enabling
B.
to
surfactin
with
yield
of
747.5
±
6.5
mg/L.
Knocking
out
ppsD
yvkC
did
not
enhance
synthesis.
Overexpression
predicted
transporter
yfiS
increased
its
titre
1060.7
89.4
mg/L,
while
overexpression
yerP,
ycxA
ycxA-efp
had
little
or
negative
effects
on
synthesis,
suggesting
YfiS
involved
efflux.
By
replacing
native
promoter
srfA
operon
encoding
synthase
three
promoters,
synthesis
significantly
reduced.
However,
knockout
global
transcriptional
regulator
codY
enhanced
1601.8
91.9
The
highest
reached
3.89
0.07
g/L,
0.63
0.02
g/g
DCW,
after
36
h
fed-batch
fermentation
5
L
fermenter.
This
study
provides
reference
for
further
understanding
constructing
microbial
cell
factories.
Frontiers in Chemistry,
Год журнала:
2024,
Номер
12
Опубликована: Янв. 19, 2024
Biosurfactants
have
garnered
increased
attention
lately
due
to
their
superiority
of
properties
over
fossil-derived
counterparts.
While
the
cost
production
remains
a
significant
hurdle
surpass
synthetic
surfactants,
biosurfactants
been
anticipated
gain
larger
market
share
in
coming
decades.
Among
these,
glycolipids,
type
low-molecular-weight
biosurfactant,
stand
out
for
efficacy
reducing
surface
and
interfacial
tension,
which
made
them
highly
sought-after
various
surfactant-related
applications.
Glycolipids
are
composed
hydrophilic
carbohydrate
moieties
linked
hydrophobic
fatty
acid
chains
through
ester
bonds
that
mainly
include
rhamnolipids,
trehalose
lipids,
sophorolipids,
mannosylerythritol
lipids.
This
review
highlights
current
landscape
glycolipids
covers
specific
glycolipid
productivity
diverse
range
products
found
global
market.
Applications
such
as
bioremediation,
food
processing,
petroleum
refining,
biomedical
uses,
increasing
agriculture
output
discussed.
Additionally,
latest
advancements
reduction
challenges
utilizing
second-generation
feedstocks
sustainable
also
thoroughly
examined.
Overall,
this
proposes
balance
between
environmental
advantages,
economic
viability,
societal
benefits
optimized
integration
secondary
biosurfactant
production.
Biotechnology Advances,
Год журнала:
2022,
Номер
60, С. 108013 - 108013
Опубликована: Июнь 23, 2022
Microbial
biosurfactants
have
attracted
the
attention
of
researchers
and
companies
for
last
decades,
as
they
are
considered
promising
candidates
to
replace
chemical
surfactants
in
numerous
applications.
Although
years,
considerable
advances
were
performed
regarding
strain
engineering
use
low-cost
substrates
order
reduce
their
production
costs,
one
main
bottlenecks
is
at
industrial
scale.
Conventional
aerobic
biosurfactant
processes
result
excessive
foaming,
due
high
agitation
aeration
rates
necessary
increase
dissolved
oxygen
concentration
allow
microbial
growth
production.
Different
approaches
been
studied
overcome
this
problem,
although
with
limited
success.
A
not
widely
explored
alternative
development
foam-free
through
anaerobic
biosurfactant-producing
microorganisms.
Surfactin,
produced
by
Bacillus
subtilis,
most
lipopeptide
biosurfactant,
powerful
known
so
far.
licheniformis
strains
produce
lichenysin,
a
which
structure
similar
surfactin.
However,
despite
its
extraordinary
surface-active
properties
potential
applications,
lichenysin
has
scarcely
studied.
According
previous
studies,
B.
better
adapted
than
could
be
good
biosurfactants.
In
review,
limitations
surfactin
under
conditions
will
analyzed,
possibility
implementing
production,
expand
market
applications
different
fields,
discussed.
