Talaromyces pinophilus exoproteome reveals high GH54 α-arabinofuranosidase production induced by soybean hulls

Biochemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 109626 - 109626

Опубликована: Янв. 1, 2025

Язык: Английский

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Effective semi-fed-batch saccharification with high lignocellulose loading using co-culture of Clostridium thermocellum and Thermobrachium celere strain A9

Frontiers in Microbiology, Год журнала: 2025, Номер 15

Опубликована: Янв. 7, 2025

Maximizing saccharification efficiency of lignocellulose and minimizing the production costs associated with enzyme requirements are crucial for sustainable biofuel production. This study presents a novel semi-fed-batch method that uses co-culture Clostridium thermocellum Thermobrachium celere strain A9 to efficiently break down high solid-loading lignocellulosic biomass without need any external enzymes. optimizes enhances glucose from alkaline-treated rice straw, representative biomass. Initially, C. T. was established treated straw loading 150 g/l, supplemented Tween 20, which enhanced enzymes stability prevented unproductive binding lignin, achieving remarkable concentration up 90.8 g/l. Subsequently, an additional 100 g/l introduced, resulting in total 140 representing 70.1% theoretical yield 250 load. In contrast, batch using initial substrate 20 resulted 55.5 only 27.7%. These results suggest co-cultivation A9, is efficient microbial saccharifying high-concentration Moreover, this approach effectively manages solids loading, efficiency, reduces enzymes, thus lowering simplifying process industrial applications.

Язык: Английский

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Cellulase from Halomonas elongata for biofuel application: enzymatic characterization and inhibition tolerance investigation

Preparative Biochemistry & Biotechnology, Год журнала: 2025, Номер unknown, С. 1 - 18

Опубликована: Янв. 21, 2025

Halophilic bacteria are promising candidates for biofuel production because of their efficient cellulose degradation. Their cellulases exhibit high activity, even in the presence inhibitors and under extreme conditions, making them ideal biorefinery applications. In this study, we isolated a strain Halomonas elongata (Kadal6) from decomposed cotton cloth on Rameshwaram seashore. Morphological, biochemical, 16S rRNA analyses revealed that Kadal6 was 99.93% similar to cellulase-producing strain, H. MH25661. The tolerance cellulase assessed through molecular docking with model MH25661 generated by I-TASSER experimentally using response surface methodology (RSM) Kadal6. A study indicated inhibition constant ethanol, hydroxymethylfurfural (HMF), furfural. Cellulase (CellHe) showed maximum rate 44.27% at 55 °C, 15% 6.5 g/L furfural HMF. enzyme retained 50% its activity these inhibitors, remained unaffected 1 HMF, although occurred 3 g/L. demonstrated significant both vitro silico, indicating potential applications harsh environments.

Язык: Английский

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Enhanced enzymatic digestibility of steam-exploded short rotation hardwood species Betula pendula and its potential for lactic acid production

Journal of Cleaner Production, Год журнала: 2025, Номер unknown, С. 145042 - 145042

Опубликована: Фев. 1, 2025

Язык: Английский

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Cost-effective fungal enzyme production and its role in methane, enhancement through biowaste hydrolysis

Journal of environmental chemical engineering, Год журнала: 2025, Номер 13(2), С. 115847 - 115847

Опубликована: Фев. 18, 2025

Язык: Английский

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