This
study
innovatively
introduced
two
deep
eutectic
solvents
(DES),
ChCl/oxalic
acid
(CO)
and
ChCl/ethylene
glycol
(CE),
into
a
34-day
co-composting
process
of
distillery
sludge
(DS)
distiller's
grains
waste
(DGW)
to
address
ammonia
emissions
challenges.
The
addition
DES
increased
dissolved
organic
carbon
by
67.60%
91.63%,
offering
more
utilizable
for
microorganisms.
SYTO9/PI
staining
enzyme
activity
tests
showed
CE
had
higher
bacterial
metabolic
levels
during
the
thermophilic
phase
than
control
(CK).
Bacterial
community
analysis
revealed
that
early
dominance
Lactobacillus
Lysinibacillus
in
accelerated
onset
phase,
reduced
pile
pH,
significantly
decreased
urease
production
reducing
Ureibacillus.
Consequently,
treatment
led
substantial
drop
73.43%
nitrogen
loss
53.84%,
compared
CK.
Besides,
fostered
abundant
functional
microbial
cooling
maturation
phases,
enhancing
degradation
humification
matter.
Regulating
the
C/N
ratio
in
composting
is
a
widely
accepted
approach
to
accelerate
humification
process
during
composting.
However,
currently,
most
traditional
study
focus
only
on
total
ratio,
and
few
research
concern
effect
of
carbon
or
nitrogen
components
at
different
bioavailable
level
key
substances
these
humification.
This
aims
clarify
mechanism
spent
mushroom
substrate
(SMS).
Two
typically
sources,
cattle
manure
(CM)
Hainan
chicken
(CH),
were
added
into
SMS
with
CM:CH
1:0,
3:1,
1:1,
1:3,
0:1,
respectively.
The
physicochemical
properties,
fluorescent
components,
humic
substance,
humus
precursors
determined
evaluate
process.
molecular
evolution
elucidated
by
excitation–emission
matrix
parallel
factor
Fourier
transform
infrared
spectrum
two-dimensional
correlation.
relationship
between
intermediate
precursor,
was
clarified.
results
indicated
that
regulating
material
bioavailability
adding
compound
N-rich
sources
an
effective
strategy
C1H3
treatment
(CM:CH
1:3)
CH
obtained
relatively
greater
properties
highest
growth
rate
acid
(HA),
which
31.96%
27.02%,
rapid
reaction
polysaccharide,
ketone,
quinone,
amide
DOM
(LCP1)
might
be
for
fast
CH.
polyphenol,
reducing
sugar
amino
originated
from
labile-carbon-proportion
I
recalcitrant-carbon-proportion
(RCP),
II
(LCP2)
RCP,
labile-nitrogen-proportion
(LNP1),
respectively,
related
HA.
result
this
provides
insight
perspective
bioavailability,
may
contribute
disposing
residue.
Regulating
nitrogen
source
composition
is
efficient
approach
to
accelerate
the
spent
mushroom
substrate
(SMS)
composting
process.
However,
currently,
most
traditional
study
only
focuses
on
total
C/N
ratio
of
initial
material.
Few
research
concerns
effect
carbon
or
components
at
different
degradable
level
and
their
corresponding
decomposed-substances
humification
This
deciphers
compare
mechanism
mixed
manure-N
sources
SMS
from
bioavailability
molecular
perspective.
Two
biodegradable
sources,
cattle
manure
(CM)
Hainan
chicken
(CH),
were
added
into
with
CM:CH
1:0,
3:1,
1:1,
1:3,
0:1,
respectively.
The
physicochemical
properties
humic
substances
determined
evaluate
compost
quality.
Coupling
analysis
spectroscopy,
fluorescence,
intermediate
precursors
conducted
characterizing
formation
process
acid.
results
indicated
that
regulating
carbon-nitrogen
nutrient
biodegradability
material
by
adding
an
effective
strategy
C1H3
(CM:CH
1:3)
CH
treatments
obtained
great
highest
growth
rate
acid
(31.96%
27.02%,
respectively).
rapid
reaction
polysaccharide,
ketone,
quinone,
amide
in
DOM
(LCP1)
might
be
key
for
fast
CH.
polyphenol,
reducing
sugar
amino
originated
labile-carbon-proportion
I
recalcitrant-carbon-proportion
(RCP),
II
(LCP2)
RCP,
labile-nitrogen-proportion
(LNP1),
respectively,
main
driving
HA.
result
this
provides
a
accelerating
elucidates
perspective
C-N
evolution,
which
may
help
enhance
disposing
efficiency
residue
practical
field.
Horticultural Science and Technology,
Год журнала:
2023,
Номер
41(5), С. 605 - 616
Опубликована: Окт. 27, 2023
High-throughput
sequencing
and
a
16S
rRNA
gene
clone
library
amplicon
analysis
were
used
to
study
the
endophytic
microbial
diversity
in
citrus
leaves
response
Xanthomonas
citri
pv.
Citri
(Xcc)
infection
an
effort
develop
biocontrol
scheme
for
canker
disease.
There
are
more
species
of
moderate
disease
(MCD)
than
severe
(SCD)
terms
both
Shannon
Simpson
index
measurements.
Taxonomy
has
shown
that
MCD
Firmicutes
group
(25.2%)
outnumber
SCD
(0.55%).
Some
can
suppress
Xcc,
but
leaves,
they
competitive
position
do
not
have
dominating
niche;
therefore,
their
population
is
greatly
decreased.
Sphingomonas
Methylobacterium,
two
genera
phylum
Proteobacteria,
growth-promoting
stress-resistant
most
plants
comprise
approximately
60%
group,
whereas
was
less
abundant.
In
conclusion,
restructures
bacterial
communities
infected
causing
community
evolve
toward
“weakening
its
spear
strengthening
shield.”
This
research
provides
support
idea
introducing
helpful
strains
advance
may
alter
relative
abundance
bacteria
given
area
hence
decrease
likelihood
by
harmful
bacteria.
The
future
technology
lies
on
functions,
elucidation
plant
processes
pathogen
occurrence,
development
biological
control
technologies.
This
study
innovatively
introduced
two
deep
eutectic
solvents
(DES),
ChCl/oxalic
acid
(CO)
and
ChCl/ethylene
glycol
(CE),
into
a
34-day
co-composting
process
of
distillery
sludge
(DS)
distiller's
grains
waste
(DGW)
to
address
ammonia
emissions
challenges.
The
addition
DES
increased
dissolved
organic
carbon
by
67.60%
91.63%,
offering
more
utilizable
for
microorganisms.
SYTO9/PI
staining
enzyme
activity
tests
showed
CE
had
higher
bacterial
metabolic
levels
during
the
thermophilic
phase
than
control
(CK).
Bacterial
community
analysis
revealed
that
early
dominance
Lactobacillus
Lysinibacillus
in
accelerated
onset
phase,
reduced
pile
pH,
significantly
decreased
urease
production
reducing
Ureibacillus.
Consequently,
treatment
led
substantial
drop
73.43%
nitrogen
loss
53.84%,
compared
CK.
Besides,
fostered
abundant
functional
microbial
cooling
maturation
phases,
enhancing
degradation
humification
matter.
