Environmental Research, Год журнала: 2024, Номер unknown, С. 120484 - 120484
Опубликована: Ноя. 1, 2024
Язык: Английский
Biochemical Engineering Journal, Год журнала: 2024, Номер 204, С. 109247 - 109247
Опубликована: Фев. 6, 2024
Язык: Английский
Процитировано
1
Fermentation, Год журнала: 2024, Номер 10(9), С. 480 - 480
Опубликована: Сен. 15, 2024
Lignocellulosic biomass (LCB) offers numerous advantages as a sustainable energy resource, such its abundance, degradability, environmental compatibility, non-toxic nature, and cost-effectiveness [...]
Язык: Английский
Процитировано
1
ACS Synthetic Biology, Год журнала: 2023, Номер 12(6), С. 1794 - 1803
Опубликована: Май 12, 2023
The GRAS (generally recognized as safe) strain Pediococcus acidilactici is well known for its antibacterial and probiotic functions. Furthermore, P. has excellent high temperature salt resistance, it an ideal host the production of food enzymes, additives, pharmaceuticals. In this regard, desirable feasible to enhance these products through metabolic engineering acidilactici. However, rare gene expression elements greatly obstruct development study, we screened constructed a library promoter-5'-UTR (PUTR) complexes in DY15 regulating at transcription translation levels. post-log phase, mRNA protein level ranges 90 native PUTRs were 0.059-2010% 0.77-245%, respectively, P32 promoter. Besides, several exhibited great stability under temperature, salt, ethanol stress. We analyzed structure obtained conserved regions promoter 5'-UTR. Based on identified core PUTRs, panel combinatorial with higher stable strongest PUTR was 853% level. Finally, applied optimize aminotransferase improve phenyllactic acid (PLA) DY15. achieved yield 950.6 mg/L, which 79.2% than wild-type strain. These results indicated that gradient strength had potential precisely achieve various goals
Язык: Английский
Процитировано
2
Environmental Research, Год журнала: 2024, Номер unknown, С. 120484 - 120484
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
0