Synthetic biology and engineering,
Год журнала:
2023,
Номер
1(1), С. 1 - 9
Опубликована: Янв. 1, 2023
Hydroxybenzoic
acids
(HBAs),
including
4-HBA,
3-HBA,
and
2-HBA,
are
valuable
platform
chemicals
for
production
of
commodity
materials
fine
chemicals.
Herein,
we
employed
metabolic
engineering
techniques
to
enhance
the
these
HBAs
in
Corynebacterium
glutamicum
ATCC
13032.
Our
approach
augmented
shikimate
pathway
eliminated
genes
associated
with
HBA
degradation,
particularly
phenol
2-monooxygenase
encoded
by
cg2966.
Increased
titers
3-HBA
4-HBA
were
achieved
via
selection
suitable
promoters
3-hydroxybenzoate
synthase
chorismate
pyruvate
lyase.
A
tac-M1
promoter
was
lyase
expression
8.3
g/L
achieved.
Efficient
2-HBA
enabled
maintaining
a
balanced
isochorismate
Consequently,
strains
KSD5-tacM1-H
KSD5-J2-PE
exhibited
levels
19.2
12.9
respectively,
using
1
L
jar
fermenter
containing
80
glucose.
Therefore,
this
engineered
strain
holds
significant
potential
other
products
derived
from
chorismate.
Environmental Technology & Innovation,
Год журнала:
2023,
Номер
31, С. 103182 - 103182
Опубликована: Май 4, 2023
Pharmaceutical
compounds
play
an
important
role
in
enhancing
the
quality
of
human
life.
They
substantially
increase
life
expectancy
humans
and
well-being
livestock.
The
expansion
global
population
has
increased
usage
pharmaceuticals
enormous
way.
This
led
to
emergence
pharmaceutical
as
environmental
pollutants
because
these
components
are
continuously
released
various
water
sources
terrestrial
ecosystems.
during
their
synthesis,
waste
from
veterinary
healthcare
sectors,
dumping
drugs
that
not
used.
known
persist
potential
even
at
lower
concentrations
can
create
serious
issues
for
ecosystems,
especially
aquatic
systems.
Various
efforts
being
made
remove
or
reduce
toxicity
Bioremediation
using
fungi
is
one
most
secure
sustainable
ways
decontaminating
polluted
environments.
With
strong
morphology
diverse
metabolic
abilities,
Fungi
employ
different
methods
including
fungal
enzymes
clear
pollutants.
Studies
have
proven
transform
into
less
toxic
components.
review
highlights
bioremediation
compounds.
Styrene
is
an
important
industrial
chemical.
Although
several
studies
have
reported
microbial
styrene
production,
the
amount
of
produced
in
batch
cultures
can
be
increased.
In
this
study,
was
using
genetically
engineered
Escherichia
coli.
First,
we
evaluated
five
types
phenylalanine
ammonia
lyases
(PALs)
from
Arabidopsis
thaliana
(AtPAL)
and
Brachypodium
distachyon
(BdPAL)
for
their
ability
to
produce
trans-cinnamic
acid
(Cin),
a
precursor.
AtPAL2-expressing
E.
coli
approximately
700
mg/L
Cin
found
that
BdPALs
could
convert
into
styrene.
To
assess
constructed
strain
co-expressed
AtPAL2
ferulic
decarboxylase
Saccharomyces
cerevisiae.
After
biphasic
culture
with
oleyl
alcohol,
production
yield
glucose
were
3.1
g/L
26.7%
(mol/mol),
respectively,
which,
best
our
knowledge,
are
highest
values
obtained
cultivation.
Thus,
applied
large–scale
Critical Reviews in Biotechnology,
Год журнала:
2024,
Номер
44(7), С. 1367 - 1385
Опубликована: Янв. 1, 2024
Spent
grains
are
one
of
the
lignocellulosic
biomasses
available
in
abundance,
discarded
by
breweries
as
waste.
The
brewing
process
generates
around
25-30%
waste
different
forms
and
spent
alone
account
for
80-85%
that
waste,
resulting
a
significant
global
volume.
Despite
containing
essential
nutrients,
i.e.,
carbohydrates,
fibers,
proteins,
fatty
acids,
lipids,
minerals,
vitamins,
efficient
economically
viable
valorization
these
is
lacking.
Microbial
fermentation
enables
grain
biomass
into
numerous
commercially
valuable
products
used
energy,
food,
healthcare,
biomaterials.
However,
still
needs
more
investigation
to
overcome
challenges,
such
transportation,
cost-effective
pretreatment,
strategy.
lower
product
cost
achieve
market
feasibility
customer
affordability.
This
review
summarizes
potential
