Synthetic biology and engineering,
Год журнала:
2023,
Номер
1(1), С. 1 - 9
Опубликована: Янв. 1, 2023
Hydroxybenzoic
acids
(HBAs),
including
4-HBA,
3-HBA,
and
2-HBA,
are
valuable
platform
chemicals
for
production
of
commodity
materials
fine
chemicals.
Herein,
we
employed
metabolic
engineering
techniques
to
enhance
the
these
HBAs
in
Corynebacterium
glutamicum
ATCC
13032.
Our
approach
augmented
shikimate
pathway
eliminated
genes
associated
with
HBA
degradation,
particularly
phenol
2-monooxygenase
encoded
by
cg2966.
Increased
titers
3-HBA
4-HBA
were
achieved
via
selection
suitable
promoters
3-hydroxybenzoate
synthase
chorismate
pyruvate
lyase.
A
tac-M1
promoter
was
lyase
expression
8.3
g/L
achieved.
Efficient
2-HBA
enabled
maintaining
a
balanced
isochorismate
Consequently,
strains
KSD5-tacM1-H
KSD5-J2-PE
exhibited
levels
19.2
12.9
respectively,
using
1
L
jar
fermenter
containing
80
glucose.
Therefore,
this
engineered
strain
holds
significant
potential
other
products
derived
from
chorismate.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Март 6, 2024
Abstract
In
the
quest
for
alternative
renewable
energy
sources,
a
new
self-assembled
hybrid
configuration
of
cellulose-coated
oil-in-water
emulsion
particles
with
yeast
was
formed.
this
research,
addition
yeasts
(
S.
cerevisiae
)
to
micro-particle
revealed
novel
self-assembly
in
which
cell
is
connected
surrounding
micro-particles.
This
may
enhance
simultaneous
saccharification
and
fermentation
process
by
substrate
channeling.
Glucose
produced
hydrolysis
cellulose
shells
coating
micro-particles,
catalyzed
cellulytic
enzymes
attached
their
coating,
directly
fermented
ethanol
are
connected.
The
results
indicate
yield
62%,
based
on
content
emulsion,
achieved
yeast/micro-particle
hybrids.
functionality
expected
serve
as
micro-reactor
cascade
biochemical
reactions
“one-pot”
consolidated
transforming
valuable
chemicals,
such
biodiesel.
L-malic
acid
(MA)
is
a
vital
platform
chemical
with
huge
market
demand
because
of
its
broad
industrial
applications.
A
cell
factory
for
MA
production
was
engineered
by
strengthening
the
intrinsic
pathway
without
inserting
foreign
genes
into
Trichoderma
reesei.
The
native
transporter
gene
in
T.
reesei
genome
characterized
(trmae1),
and
overexpression
significantly
improved
production,
which
increased
from
2
to
56.24
g/L.
Native
pyruvate
carboxylase,
malate
dehydrogenase,
malic
enzyme,
glucose
were
overexpressed
further
improve
titer
yield
production.
Fungal
morphology
adapted
produce
fermenter
deleting
gul1.
235.8
g/L
produced
final
strain
5-L
1.48
mol
per
productivity
1.23
g/L/h.
This
study
provides
novel
insights
understanding
remodeling
synthesis
pathway.
Synthetic biology and engineering,
Год журнала:
2023,
Номер
1(1), С. 1 - 9
Опубликована: Янв. 1, 2023
Hydroxybenzoic
acids
(HBAs),
including
4-HBA,
3-HBA,
and
2-HBA,
are
valuable
platform
chemicals
for
production
of
commodity
materials
fine
chemicals.
Herein,
we
employed
metabolic
engineering
techniques
to
enhance
the
these
HBAs
in
Corynebacterium
glutamicum
ATCC
13032.
Our
approach
augmented
shikimate
pathway
eliminated
genes
associated
with
HBA
degradation,
particularly
phenol
2-monooxygenase
encoded
by
cg2966.
Increased
titers
3-HBA
4-HBA
were
achieved
via
selection
suitable
promoters
3-hydroxybenzoate
synthase
chorismate
pyruvate
lyase.
A
tac-M1
promoter
was
lyase
expression
8.3
g/L
achieved.
Efficient
2-HBA
enabled
maintaining
a
balanced
isochorismate
Consequently,
strains
KSD5-tacM1-H
KSD5-J2-PE
exhibited
levels
19.2
12.9
respectively,
using
1
L
jar
fermenter
containing
80
glucose.
Therefore,
this
engineered
strain
holds
significant
potential
other
products
derived
from
chorismate.
