Abstract
An
estimated
258
million
tons
of
plastic
enter
the
soil
annually.
Joining
persistent
types
microplastic
(MP),
there
will
be
an
increasing
demand
for
biodegradable
plastics.
There
are
still
many
unknowns
about
pollution
by
either
type,
and
one
large
gap
is
fate
composition
dissolved
organic
matter
(DOM)
released
from
MPs
as
well
how
they
interact
with
microbiomes
in
agricultural
systems.
In
this
study,
polyethylene
MPs,
photoaged
to
different
degrees,
virgin
polylactic
acid
were
added
at
levels
incubated
100
days
address
knowledge
gap.
We
find
that,
upon
MP
addition,
labile
components
low
aromaticity
degraded
transformed,
resulting
increased
oxidation
degree,
reduced
molecular
diversity,
changed
nitrogen
sulfur
contents
DOM.
Terephthalate,
acetate,
oxalate,
L-lactate
DOM
4-nitrophenol,
propanoate,
nitrate
major
molecules
available
microbiomes.
The
bacteria
involved
metabolism
mainly
concentrated
Proteobacteria,
Actinobacteriota,
Bacteroidota,
fungi
Ascomycota
Basidiomycota.
Our
study
provides
in-depth
understanding
microbial
transformation
its
effects
evolution
soils.
Abstract
Biochar
and
organic
fertilizer
substitution
have
been
utilized
to
improve
crop
yields
promote
sustainable
agricultural
development.
However,
few
studies
focused
on
how
biochar
regulate
the
diversities
of
bacterial
meta-communities
sub-communities
(i.e.,
habitat
specialists
generalists)
within
soil
aggregates
Loess
Plateau.
A
field
experiment,
containing
six
treatments,
namely
no
(CK),
plus
10
4
kg
ha
−1
(CK
+
B),
traditional
N
(TF,
150
),
(TF
replacing
(OF),
(OF
was
adopted
explore
influences
size
distribution,
nutrient
levels,
extracellular
enzymatic
stoichiometry,
meta-community
sub-community
diversities,
structures,
ecological
networks,
assembly
processes
bulk
soil,
large
macro-aggregates
(>
2
mm),
small
(0.25–2
micro-aggregates
(<
0.25
mm)
in
proso
millet
farmland.
Results
showed
that
compared
CK
B
treatment,
OF
treatment
enhanced
distribution
while
decreasing
micro-aggregates.
also
markedly
elevated
contents
total
nitrogen
(TN),
carbon
(SOC),
nitrate
(NO
3
−
-N),
ammonium
(NH
as
well
activities
C-,
N-,
P-acquiring
enzymes,
limitation,
reduced
limitation
macro-aggregates.
Compared
treatments
significantly
increased
observed
OTUs
specialist
communities
macroaggregates,
having
effect
generalist
communities.
The
impacts
additions,
aggregates,
community
structure
composition
were
greater
than
those
generalists.
RDA
analysis
NH
-N
content
had
a
significant
influence
three
aggregates.
Ecological
network
indicated
whole
complexity
stability
treatment.
Meanwhile,
with
amendment
more
complex
tighter
without
biochar.
Null-model
regimes
rarely
affected
mainly
determined
by
stochastic
process.
These
observations
offer
theoretical
basis
for
understanding
intrinsic
relationships
between
microbial
regulated
fertilization
semi-arid
region.
Microorganisms,
Год журнала:
2025,
Номер
13(3), С. 571 - 571
Опубликована: Март 3, 2025
Aerobic
composting
is
widely
used
for
the
degradation
of
organic
matter,
simultaneously
reducing
presence
antibiotic
resistance
genes
(ARGs)
in
swine
manure.
However,
phenomenon
abundance
rebound
or
even
enrichment
ARGs
still
a
problem.
The
effect
and
mechanism
humus
soil
(Hs)
on
ARG
reduction
by
adding
it
into
piles
(0%
control
group
(CK);
10%
S1
group;
20%
S2
30%
S3
group)
after
thermophilic
phase
was
investigated.
results
indicated
that
Hs
promoted
matter
nitrogen
loss.
During
days
15–36,
greatest
69.91%
total
observed
S2,
while
rebounded
222.75%
CK
decreased
only
13.71%
S3.
With
addition,
85.42%
mobile
genetic
elements
(MGEs)
100%
removal
rates
aadA5,
aadA9,
sul1,
sul2,
tetX
were
achieved.
Moreover,
addition
immediately
changed
bacterial
community
structure
substrate
varied
successional
direction
treatments.
Additionally,
significantly
positive
correlations
(|r|
>
0.6;
p
<
0.05)
found
between
top
20
genera
ARGs.
potential
host
bacteria
from
Lactobacillus,
Fermentimonas,
Pusillimonas,
Ruminofilibacter
to
Romboutsia,
Streptococcus
highlighting
shift
driven
Hs,
which,
turn,
influenced
variations
This
study
verified
feasibility
inhibiting
effectively
influencing
microecological
niche
pile,
offering
an
approach
promoting
animal
wastes.
