Polymer,
Год журнала:
2023,
Номер
293, С. 126659 - 126659
Опубликована: Дек. 30, 2023
The
structural
evolution
of
poly(ethylene
vanillate)
(PEV)
crystals,
after
isothermal
crystallization
at
Tc
=
180
°C,
was
explored
by
synchrotron
wide-angle
and
small-angle
X-ray
scattering
(WAXS
SAXS)
measurements.
WAXS/SAXS
analyses
proved
that
an
additional
more
perfect
crystal
population
starts
to
grow
exactly
in
correspondence
the
exotherm
displayed
specific
heat
capacity
curve.
study
ascertained
two
different
reorganization/recrystallization
mechanisms
occur
upon
heating:
crystals
originate
from
a
recrystallization
process,
whereas
original
undergo
small
progressive
perfection
maybe
without
previous
complete
fusion.
Deconvolution
double
Lorentz-corrected
SAXS
profiles
performed
calculate
temperature
lamellar
thickness
for
populations.
populations
appear
differ
substantially
amorphous
thicknesses.
Hypotheses
on
relative
location
stacks
as
well
possible
thermodynamic
reason
triggers
formation
have
been
formulated.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(8), С. 4217 - 4224
Опубликована: Фев. 15, 2024
Vanillic
acid
(VA),
as
a
plant-derived
phenolic
compound,
has
widespread
applications
and
good
market
prospects.
However,
the
traditional
production
process
cannot
meet
demand.
In
this
study,
Pseudomonas
putida
KT2440
was
used
for
de
novo
biosynthesis
of
VA.
Multiple
metabolic
engineering
strategies
were
applied
to
construct
these
P.
putida-based
cell
factories,
including
introduction
Hs-OMTopt,
cofactor
S-adenosylmethionine
supply
pathway
through
overexpression
metX
metH,
reforming
solubility
increasing
second
copy
optimization
fermentation
medium.
The
resulting
strain,
XCS17,
biosynthesized
5.4
g/L
VA
from
glucose
in
fed-batch
system;
is
highest
titer
reported
up
recently.
This
study
showed
that
robust
platform
achieving
effective
acids.
