Microorganisms,
Год журнала:
2024,
Номер
12(11), С. 2135 - 2135
Опубликована: Окт. 24, 2024
Polyhydroxyalkanoates
(PHAs)
are
polyesters
synthesized
as
a
carbon
and
energy
reserve
material
by
wide
number
of
bacteria.
These
polymers
characterized
their
thermoplastic
properties
similar
to
those
plastics
derived
from
the
petrochemical
industry,
such
polyethylene
polypropylene.
PHAs
widely
used
in
medical
field
have
potential
be
other
applications
due
biocompatibility
biodegradability.
Among
PHAs,
P(3HB-
International Journal of Polymer Science,
Год журнала:
2024,
Номер
2024, С. 1 - 17
Опубликована: Янв. 19, 2024
Polyhydroxyalkanoates
(PHAs)
are
biodegradable
and
biocompatible
polymers
that
produced
by
microorganisms
as
storage
materials
under
limited
nutrition
excess
carbon.
These
PHAs
have
been
found
to
be
ideal
for
replacing
synthetic
plastics
use
in
packaging
biomedical
applications.
In
this
study,
an
alkaliphilic
moderately
halophilic
bacterium
Halomonas
alkalicola
Ext
was
isolated
from
Lake
Simbi
Nyaima
western
Kenya
investigated
PHA
production.
Sudan
Black
B
Nile
Red
A
staining
showed
had
distinct
ability
accumulation
of
PHAs.
To
optimize
production,
the
grown
submerged
fermentation
varying
culture
conditions
different
sources
concentrations
carbon
nitrogen.
With
one-factor-at-a-time
(OFTA)
approach,
optimal
yields
were
obtained
after
72
hours
at
a
pH
10.0,
temperature
35°C,
2.5%
(w/v)
NaCl.
The
yielded
highest
biomass,
amounts
on
2%
galactose
0.1%
ammonium
sulfate
nitrogen,
respectively.
record
yield
0.071
g
g-1
with
titer
achieved
3.397
g/L
equivalent
41.8%
content.
Using
response
surface
methodology,
increased
1.5%
1.44
g/L,
while
content
improved
1.1-fold
45.57%.
Polymer
analysis
revealed
extracted
poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
(PHBV)
(
id="M2">3−HB:3−HV=92:8
)
two
copolymer
subunits
3-hydroxyvaryrate
(3-HB)
3-hydroxybutyrate
(3-HV).
attained
efficient
conversion
into
PHBV
high
salinity
alkalinity
conditions.
