Advances in the bioproduction of d-allulose: A comprehensive review of current status and future prospects

Food Research International, Год журнала: 2025, Номер 202, С. 115767 - 115767

Опубликована: Янв. 18, 2025

Язык: Английский

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Rational Design of Spontaneous Self-Cyclization Enzymes In Vivo and In Vitro with Improved Thermal Tolerance and Activity

ACS Catalysis, Год журнала: 2024, Номер 14(7), С. 5469 - 5480

Опубликована: Март 28, 2024

Enzymes have selectivity, require mild catalytic conditions, and are important cornerstones in many industrial processes. Protein self-cyclization has opened up the possibility of preserving fragile enzymes during long-term high-temperature catalysis. However, mechanism for improvement thermal tolerance not been elucidated, severely limiting their applications. Herein, we provide a strategy rational design fusion proteins based on structural analysis to obtain cyclized with improved properties. First, constructed that preferentially translated SpyCatcher (CBT) or SpyTag (TBC), both which could form stable single significantly tolerance. Especially, half-life TBC obtained by modifying N-terminal at 40 °C was 10.83 times wild enzymes. Structural revealed terminus protein, SpyCatcher, folded into conformation adversely affected stability. In addition, structure pocket orientation residues CBT were different from those wild-type enzymes, led decrease activity. These conclusions confirmed when another enzyme, sucrose phosphorylase, cyclized. Finally, glucosidase also superior strain preparation ginsenoside F1 high titers as whole-cell catalyst extended use. conclusion, demonstrated first time conjugated long oligopeptide activity stability It necessary translate units less steric hindrance reduce impact protein structure. The provides simple effective modification poor tolerance, providing considerable prospects biosynthesis vivo vitro.

Язык: Английский

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Green biotechnological synthesis of rare sugars/alcohols: D-allulose, Allitol, D-tagatose, L-xylulose, l-ribose

Food Research International, Год журнала: 2025, Номер 206, С. 116058 - 116058

Опубликована: Фев. 25, 2025

Язык: Английский

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A circular economy approach for harnessing inorganic and organic carbons coupled with waste remediation using Chlorella minutissima for sustainable biodiesel production

Journal of environmental chemical engineering, Год журнала: 2025, Номер unknown, С. 117069 - 117069

Опубликована: Май 1, 2025

Язык: Английский

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Recent advances in discovery, protein engineering, and heterologous production of ketose 3-epimerase for rare sugar biosynthesis

Trends in Food Science & Technology, Год журнала: 2024, Номер 149, С. 104552 - 104552

Опубликована: Май 20, 2024

Язык: Английский

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Advances in the biosynthesis of D-allulose

World Journal of Microbiology and Biotechnology, Год журнала: 2024, Номер 40(12)

Опубликована: Ноя. 2, 2024

Язык: Английский

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Advancements in the Heterologous Expression of Sucrose Phosphorylase and Its Molecular Modification for the Synthesis of Glycosylated Products

Molecules, Год журнала: 2024, Номер 29(17), С. 4086 - 4086

Опубликована: Авг. 28, 2024

Sucrose phosphorylase (SPase), a member of the glycoside hydrolase GH13 family, possesses ability to catalyze hydrolysis sucrose generate α-glucose-1-phosphate and can also glycosylate diverse substrates, showcasing wide substrate specificity. This enzyme has found extensive utility in fields food, medicine, cosmetics, garnered significant attention as focal point research transglycosylation enzymes. Nevertheless, SPase encounters numerous obstacles industrial settings, including low yield, inadequate thermal stability, mixed regioselectivity, limited activity. In-depth exploration efficient expression strategies molecular modifications based on crystal structure functional information is now critical priority. paper systematically reviews source microorganisms, structure, catalytic mechanism SPase, summarizes heterologous systems hosts vectors, examines application modification progress synthesizing typical glycosylated products. Additionally, it anticipates broad prospects production related fields, laying groundwork for its engineering application.

Язык: Английский

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Green directional conversion of food waste into glycerol in a two-step differentiation enzymolysis and facultative fermentation using Saccharomyces cerevisiae: Performance assessment and mechanism

Biomass and Bioenergy, Год журнала: 2024, Номер 189, С. 107360 - 107360

Опубликована: Авг. 31, 2024

Язык: Английский

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Semi-Rational Engineering of Aldoxime Dehydratase for Conducting a Chemoenzymatic Sequence to Prepare 2-Furonitrile from Xylose

ACS Sustainable Resource Management, Год журнала: 2024, Номер 1(10), С. 2214 - 2224

Опубликована: Окт. 15, 2024

Exploiting efficient methods for converting biomass-based resources into high-value-added chemicals has attracted extensive interest in sustainable development the chemical industry. Here, we have developed a chemoenzymatic sequence synthesizing 2-furonitrile (2-FN) from xylose, biobased five-carbon monosaccharide derived agricultural waste. Firstly, 1,2-dichloroethane (DCE)/H2O biphasic system (1:1, v/v) was adopted to produce 2-furaldehyde oxime (2-FOx) xylose by integrating two steps of dehydration and oximation one-pot using temporal compartmentalization strategy, resulting yield 2-FOx over 78%. Secondly, catalytic efficiency aldoxime dehydratase Pseudomonas putida F1 (OxdF1) significantly improved engineering substrate access tunnel distal residue. The activity an optimal mutant L318I–N266S reached 3.94 U·mg–1 towards 2-FOx, approximately 6 times higher than that wild-type OxdF1 (0.65 U·mg–1). Consequently, 2-FN prepared 400 mL reaction mixture at room temperature continuous feeding strategy. After 1.5 h, 100 mM completely converted with space-time 6.2 g·L–1·h–1. process proposed alternative strategy materials under mild conditions without highly toxic cyanide.

Язык: Английский

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Fabricating compartmented nanoreactor via protein self-regulated biomimetic mineralization for multienzyme immobilization

Advanced Composites and Hybrid Materials, Год журнала: 2024, Номер 7(6)

Опубликована: Ноя. 18, 2024

Язык: Английский

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