Utilizing a Novel Halotolerant Bordetella Bacterium Combined with Co-Metabolites to Boost the Degradation of P-Nitrophenol in High-Salinity Wastewater DOI Open Access
Lei Qin,

Haorui Li,

Yingyu Tan

и другие.

Water, Год журнала: 2024, Номер 16(23), С. 3360 - 3360

Опубликована: Ноя. 22, 2024

A novel strain capable of fully utilizing p-nitrophenol (PNP) as the sole carbon source under high-salinity conditions was isolated from sediments wastewater discharged an aquaculture company. The identification Bordetella sp. confirmed by analyzing its morphological, physiological, and biochemical traits in conjunction with 16S rDNA sequence. Furthermore, pantothenic acid, serving a for co-metabolites, could significantly enhance biodegradation process tricarboxylic acid (TCA) cycle. Under optimal growth at temperature 30 °C, pH 8.0, aeration 0.32 m3·(m3·min)−1 salinity 3% (NaCl, w/v), degradation rate 350 mg·L−1 PNP increased 60.8% to 85.9% within 72 h after adding 12-liter bioreactor. intermediate products process, analyzed via GC/MS, were determined be hydroquinone, which suggests that pathway bacterium involves breakdown hydroquinone. Benefits have been derived microorganism’s tolerance high concentrations, coupled superior performance, offering new insights research basis efficient biological treatment wastewater.

Язык: Английский

Efficient Synthesis of Vitamin B5 in Escherichia coli by Engineering Ketopantoate Hydroxymethyltransferase and Cofactor Supply DOI

Kun Qiu,

Fuqiang Song, Ke Wang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

d-pantothenic acid (d-PA), also known as vitamin B5, is an essential precursor of coenzyme A and plays a crucial role in maintaining the physiological functions organisms. Ketopantoate hydroxymethyltransferase (PanB), encoded by panB gene, serves key rate-limiting enzyme d-PA synthesis. Additionally, catalytic function PanB requires cofactor 5,10-methylenetetrahydrofolate (5,10-CH2–THF). This study aimed to increase production engineering ketopantoate supply. The transcription factor bhsA that restricts was screened identified through applications. Subsequently, coexpressed with PanC regulate expression. Furthermore, highly mutant PanBMV123I/K124W generated Km/Kcat algorithm prediction engineering, leading 2.5-fold production. de novo synthesis pathway 5,10-CH2–THF enhanced, whereas its degradation suppressed improve Then, extracellular transport enhanced introducing transporter PanT from Streptococcus intermedius. plasmid-free strain DPA23 produced 78.48 g/L 5-L bioreactor, productivity 2.69 g/L/h after 24 h glucose yield 0.54 g/g. These strategies provided reference for constructing microbial cell factories derivatives.

Язык: Английский

Процитировано

2
Balancing the (R)-pantoate and β-alanine pathway of Escherichia coli by modular co-culture engineering to produce D-pantothenic acid from glycerol DOI

Hai‐Bin Qin,

Junping Zhou, Kun Niu

и другие.

Food Bioscience, Год журнала: 2025, Номер unknown, С. 105964 - 105964

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Metabolic engineering of Escherichia coli for the production of d-panthenol from 3-aminopropanol and glucose DOI
Junping Zhou, Zheng Zhang,

Xinyuan Xin

и другие.

Green Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

The novel de novo biosynthesis of d -panthenol from glucose and 3-aminopropanol was realized in Escherichia coli for the first time.

Язык: Английский

Процитировано

0
Dynamic Regulation of the l-Proline Pathway for Efficient l-Arginine Production in Escherichia coli DOI
Lijuan Wang, Kun Yang, Zhi Zhang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

l-Arginine, a semiessential amino acid crucial for human health, has broad applications in cosmetics, nutraceuticals, feed, and pharmaceuticals. In this study, we developed an Escherichia coli strain with enhanced l-arginine production by deregulating negative feedback, enhancing the synthesis pathway, increasing precursor cofactor availability. The engineered achieved titers of 6.41 g/L shake flasks 63.9 yield 0.31 g/g glucose 5 L fermenter. Blocking competitive l-proline pathway elevated titer to 9.36 but reduced biomass. To fine-tune without exogenous l-proline, dynamic regulatory method proB gene control. final strain, harboring driven temperature-sensitive promoter, 65.6 0.42 Balancing growth through regulation presents viable strategy refining bioproduction.

Язык: Английский

Процитировано

0
Intelligent biomanufacturing of water-soluble vitamins DOI
Fuqiang Song, Heng Zhang,

Zhijie Qin

и другие.

Trends in biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Utilizing a Novel Halotolerant Bordetella Bacterium Combined with Co-Metabolites to Boost the Degradation of P-Nitrophenol in High-Salinity Wastewater DOI Open Access
Lei Qin,

Haorui Li,

Yingyu Tan

и другие.

Water, Год журнала: 2024, Номер 16(23), С. 3360 - 3360

Опубликована: Ноя. 22, 2024

A novel strain capable of fully utilizing p-nitrophenol (PNP) as the sole carbon source under high-salinity conditions was isolated from sediments wastewater discharged an aquaculture company. The identification Bordetella sp. confirmed by analyzing its morphological, physiological, and biochemical traits in conjunction with 16S rDNA sequence. Furthermore, pantothenic acid, serving a for co-metabolites, could significantly enhance biodegradation process tricarboxylic acid (TCA) cycle. Under optimal growth at temperature 30 °C, pH 8.0, aeration 0.32 m3·(m3·min)−1 salinity 3% (NaCl, w/v), degradation rate 350 mg·L−1 PNP increased 60.8% to 85.9% within 72 h after adding 12-liter bioreactor. intermediate products process, analyzed via GC/MS, were determined be hydroquinone, which suggests that pathway bacterium involves breakdown hydroquinone. Benefits have been derived microorganism’s tolerance high concentrations, coupled superior performance, offering new insights research basis efficient biological treatment wastewater.

Язык: Английский

Процитировано

0