Cultivation of filamentous fungi in airlift bioreactors: advantages and disadvantages

Applied Microbiology and Biotechnology, Год журнала: 2025, Номер 109(1)

Опубликована: Фев. 10, 2025

Abstract Filamentous fungi or mycelia are a valuable bioresource to produce several biomolecules and enzymes, especially because of their biodegradation potential for key role enablers circular bioeconomy. can be grown in submerged cultivation maximise the volumetric productivity bioprocess, instead using more established time-consuming solid-state cultivation. Multicellular sensitive shear stresses induced by mechanical agitation, this aspect greatly affects morphology (pelletisation) connected productivity. An efficient compromise is growth filamentous airlift bioreactors (ALR) where oxygen transfer (K L a) optimal, but stress reduced. In review, we critically analysed advantages disadvantages ALR-based fungi, comparing these also with stirred tank reactors bubble column reactors; focused on scientific literature that highlights findings both production enzymes myco-biomass ALR; included studies control pelletisation fungal biomass batch semi-continuous cultivation, highlighting interlinked hydrodynamics; finally, regarding modifications ALR order enhance production. Key points • prolonged continuous fungi. show optimal gas hold-up K an airflow has high superficial velocity critical diameter (1–6 mm). Suspended aggregates (pellet) maintain fluidised motion if size/density controlled.

Язык: Английский

Exploring the Fermentation Potential of Kluyveromyces marxianus NS127 for Single-Cell Protein Production

Fermentation, Год журнала: 2025, Номер 11(2), С. 70 - 70

Опубликована: Фев. 2, 2025

Kluyveromyces marxianus is a food-grade yeast known for its diverse beneficial traits, making it an attractive candidate both food and biotechnology applications. This study explores the potential of as promising alternative protein source single-cell (SCP) production. Various strains were isolated screened from traditional fermented dairy products, with NS127 identified most strain due to superior growth characteristics, high SCP yield, environmental tolerance. Notably, demonstrated significant substrate conversion capacity biomass yield 0.63 g biomass/g molasses, achieving dry concentration 66.64 g/L 28.37 g/L. The extracted exhibited excellent solubility (62.55%) emulsification properties (13.15 m2/g) under neutral conditions, alongside foaming stability (93.70–99.20%) across broad pH range (3–11). These results underscore viable provide solid theoretical foundation industrial application.

Язык: Английский

From microbial proteins to cultivated meat for alternative meat-like products: a review on sustainable fermentation approaches

Journal of Biological Engineering, Год журнала: 2025, Номер 19(1)

Опубликована: Май 14, 2025

Язык: Английский