Metabolic Engineering of Zymomonas mobilis for Xylonic Acid Production from Lignocellulosic Hydrolysate

Fermentation, Год журнала: 2025, Номер 11(3), С. 141 - 141

Опубликована: Март 13, 2025

Bio-based xylonic acid produced from inexpensive lignocellulosic biomass has enormous market potential and enhances the overall economic benefits of biorefinery processes. In this study, introduction genes encoding xylose dehydrogenase driven by promoter Ppdc into Z. mobilis using a plasmid vector resulted in accumulation at titer 16.8 ± 1.6 g/L. To achieve stable production, gene cassette for production was integrated genome chromosomal locus ZMO0038 ZMO1650 endogenous type I-F CRISPR-Cas system. The resulting recombinant strain XA3 reduced to 12.2 0.56 g/L, which could be copy number difference between integration. Oxygen content then identified key factor production. further increase capability, strain, XA9, with five copies constructed integrating ZMO1094, ZMO1547, ZMO1577 on basis XA3. increased 51.9 0.1 g/L maximum yield 1.10 g/g, is close theoretical pure sugar medium. addition, XA9 genetically can produce 16.2 0.14 1.03 0.01 g/g hydrolysate. Our study thus hydrolysate, demonstrating capability as chassis biochemical

Язык: Английский

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Impact of Oxalic Acid Pretreatment on the Solubility and Fermentability of Hemicelluloses in Brewer’s Spent Grain

Industrial Biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Язык: Английский

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Physiological Functions of the By-Products of Passion Fruit: Processing, Characteristics and Their Applications in Food Product Development

Foods, Год журнала: 2025, Номер 14(9), С. 1643 - 1643

Опубликована: Май 7, 2025

The by-products of passion fruit are typically discarded during processing, contributing to resource waste and environmental harm. These residues rich in dietary fiber polyphenols, compounds linked health benefits, including blood sugar regulation, improved lipid profiles, gut microbiome balance, weight management. Beyond their nutritional value, these possess dual functional roles food systems: bioactive components act as natural fortifiers health-promoting agents. Recent studies indicate they can enhance quality by improving water retention texture while serving prebiotics promote beneficial bacteria growth. This functionality supports both innovation metabolic health, particularly reducing post-meal spikes. To advance research industry applications, this review synthesizes recent findings on the properties use products such dairy, pasta, meat. analysis aims guide sustainable utilization underrated resources expand role development.

Язык: Английский

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Valorising hemicellulosic fraction of corncobs concomitantly into ethanol and xylitol using Candida tropicalis Y6

Waste Management Bulletin, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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