Small Extracellular Vesicles Detection by Dielectrophoresis-Based Microfluidic Chip Filled with Transparent Antibody-Conjugated Microbeads for Breast Cancer Diagnosis

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 7, 2025

Small extracellular vesicles (sEVs) are ideal biomarkers for early diagnosis of tumors, but their low density and small size make them difficult to enrich detect in body fluid samples. This study combined dielectrophoresis (DEP) with immunoaffinity methods proposed a novel, fast, efficient acDEP-sEV immunochip, which by filling the chip reaction chamber antibody-conjugated microbeads, nonuniform electric field can be constructed, laminar flow disrupted, mass transfer efficiency improved, fluorescence signals focused, ultimately achieving rapid, sensitive, adjustable sEVs capture detection. method only requires 20–50 μL plasma sample liquid biopsy less than 35 min. We analyzed total sEVs, EpCAM, MUC1 positive clinical samples, found that evaluation multiple has extremely high sensitivity, accuracy, specificity. Meanwhile, also achieved limit detection 54 sEV/μL. hybrid approach provides new strategy rapid sensitive cancer other diseases based on sEVs.

Язык: Английский

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Hybridization-based sensor with large dynamic range for detection of circulating tumor DNA in clinical samples

Biosensors and Bioelectronics, Год журнала: 2025, Номер 281, С. 117342 - 117342

Опубликована: Апрель 10, 2025

Язык: Английский

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A novel method and classification criteria for analyzing urine turbidity and its relationship with urine dry chemical parameters

PLoS ONE, Год журнала: 2025, Номер 20(5), С. e0323351 - e0323351

Опубликована: Май 7, 2025

Background Urine turbidity is a significant diagnostic marker for early screening of urinary tract infections, kidney stones, and other related conditions. However, current methods analyzing urine often rely on subjective assessments. This study aims to investigate the relationship between color values measured by spectrophotometer, providing an objective quantification method both color, while also exploring underlying causes turbidity. Methods A cross-sectional was conducted among newly enrolled university students undergoing physical examination in Beijing. Basic demographic information morning samples were collected. assessed using human visual evaluation chemical analyzer, CIE L*a*b* (International Commission illumination) spectrophotometer. Routine performed Correlations turbidity, dry parameters analyzed. Results total 1220 participants (68.7% female, mean age: 23.66 years) included study. Spearman correlation analysis showed that significantly negatively correlated with L* (lightness) positively a* (redness) b* (yellowness). Regression identified as most affected parameter (standardized coefficient β=-1.030, p < 0.05). Receiver operating characteristic (ROC) highly effective distinguishing different levels, 89.165 achieving excellent sensitivity specificity (AUC = 0.984) 96% accuracy identifying turbid urine. In addition, specific gravity, protein, ( 0.05), its pH nonlinear. These findings suggest multiple factors collectively influence Conclusion provides novel approach assessing advancing modernization practices traditional medicine.

Язык: Английский

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Distance-based α-amylase biosensor fabricated with amylopectin-coated mesoporous membrane

Chinese Chemical Letters, Год журнала: 2023, Номер 35(1), С. 108462 - 108462

Опубликована: Апрель 16, 2023

Язык: Английский

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Identification of miR-106b-5p, miR-601, and miR-760 Expression and Their Clinical Values in Non-Small Cell Lung Cancer (NSCLC) Patients' Serum

Pathology - Research and Practice, Год журнала: 2023, Номер 248, С. 154663 - 154663

Опубликована: Июль 6, 2023

Язык: Английский

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