Block-Polymer-Restricted Sub-nanometer Pt Nanoclusters Nanozyme-Enhanced Immunoassay for Monitoring of Cardiac Troponin I

Analytical Chemistry, Год журнала: 2023, Номер 95(38), С. 14494 - 14501

Опубликована: Сен. 14, 2023

Noble-metal nanozymes have demonstrated great potential in various fields. However, aggregation of single-particle nanoparticles severely affects their exposed catalytically active sites to the extent exhibiting weak enzyme-like activity. Here, we present an organic block surfactant (polyvinylpyrrolidone, PVP) construct monodisperse water-stable Pt nanoclusters (Pt NCs) for enhanced immunoassay cardiac troponin I (cTnI). The PVP-modified NC nanozyme exhibited up 16.3 U mg-1 peroxidase-mimicking activity, which was mainly attributed ligand modification on surface and electron-absorbing effect NCs. NCs a lower OH-transition potential, as determined by density functional theory. Under optimized experimental conditions, strategy ultrawide dynamic response range 0.005-50 ng mL-1 cTnI targets with detection limit 1.3 pg mL-1, far superior some reported test protocols. This work provides designable pathway design artificial enzymes high activity further expand practical enzyme alternatives.

Язык: Английский

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Photoelectrochemical sensors based on paper and their emerging applications in point-of-care testing

Biosensors and Bioelectronics, Год журнала: 2023, Номер 236, С. 115400 - 115400

Опубликована: Май 28, 2023

Язык: Английский

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Shaping the Future of the Neurotransmitter Sensor: Tailored CdS Nanostructures for State-of-the-Art Self-Powered Photoelectrochemical Devices

ACS Sensors, Год журнала: 2024, Номер 9(5), С. 2684 - 2694

Опубликована: Май 2, 2024

Semiconductor-based photoelectrochemical (PEC) test protocols offer a viable solution for developing efficient individual health monitoring by converting light and chemical energy into electrical signals. However, slow reaction kinetics electron–hole complexation at the interface limit their practical application. Here, we reported triple-engineered CdS nanohierarchical structures (CdS NHs) modification scheme including morphology, defective states, heterogeneous structure to achieve precise of neurotransmitter dopamine (DA) in plasma noninvasive body fluids. By precisely manipulating Cd–S precursor, achieved control over ternary NHs obtained well-defined layered self-assembled through surface carbon treatment. The integration defect states thin layer effectively established carrier directional transfer pathways, thereby enhancing sites improving conversion efficiency. microelectrode fabricated demonstrated remarkable negative response toward DA, enabling development miniature self-powered PEC device quantification human saliva. Additionally, utilization density functional theory calculations elucidated structural characteristics DA state CdS, thus establishing crucial theoretical groundwork optimizing polymerization process DA. present study offers potential engineering approach high efficiency semiconductors as well proposing novel concept designing sensitive testing strategies.

Язык: Английский

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Smartphone-based point-of-care photoelectrochemical immunoassay coupling with ascorbic acid-triggered photocurrent-polarity conversion switching

Biosensors and Bioelectronics, Год журнала: 2024, Номер 267, С. 116749 - 116749

Опубликована: Сен. 6, 2024

Язык: Английский

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Recent advances in metal–organic framework-based photoelectrochemical and electrochemiluminescence biosensors

The Analyst, Год журнала: 2023, Номер 148(10), С. 2200 - 2213

Опубликована: Янв. 1, 2023

This review summarizes recent advances on metal-organic frameworks for the applications in photoelectrochemical and electrochemiluminescence biosensors.

Язык: Английский

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Smartphone-Based Portable Bio-Chemical Sensors: Exploring Recent Advancements

Chemosensors, Год журнала: 2023, Номер 11(9), С. 468 - 468

Опубликована: Авг. 22, 2023

Traditionally, analytical chemistry and diagnosis relied on wet laboratories skilled professionals utilizing sophisticated instruments for sample handling analysis. However, with the development of novel materials sensing techniques, there has been a significant shift towards use standalone sensors, allowing tests to be conducted on-site or even in real time, leading cost- time-efficiency. With their widespread adoption globally, smartphones have emerged as an ideal platform such boasting extensive sensor capabilities, advanced processing power, communication functionalities. Smartphone-based assays make optical electrochemical built-in cameras, ambient light other features sensing, while micro-USB port, Bluetooth, wireless connection facilitate data transmission analog voltage application sensing. Previous overview papers explored smartphone-based specific domains; this review provides comprehensive examination recent advancements encompassing both methods. The fundamental principles these sensors implementation using smartphones, showcases applications, presents innovative designs that take advantage inherent functionalities capabilities smartphones. concludes by offering outlook prospects includes reflective section emphasizing potential impact chemical biological analyses. This resource aims provide information researchers practitioners interested cutting-edge methodologies.

Язык: Английский

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Recent advances on portable photoelectrochemical biosensors for diagnostics

Electroanalysis, Год журнала: 2023, Номер 35(12)

Опубликована: Окт. 14, 2023

Abstract Efficient and accurate diagnostic methods are important for human health monitoring prevention of major diseases, so the establishment efficient convenient testing clinical diagnosis is urgent. Photoelectrochemical (PEC) biosensors have attracted much attention because their high sensitivity, low detection background, especially advantages combining photoexcitation electrochemical detection. With rapid development integrated circuit technology many wireless transmissions highly aggregated portable micro devices manufactured, its small size, easy to carry, ability visualize results test shown be potential application such devices. This review aims cover recent advances in PEC biosensing within field detection, including principles biosensors, common photovoltaic materials, scalable fabrication techniques biosensing. Future prospects also discussed.

Язык: Английский

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Recent advances in metal–organic frameworks as emerging platforms for immunoassays

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 171, С. 117520 - 117520

Опубликована: Янв. 4, 2024

Язык: Английский

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One-pot synthesis of stable and fast Bi2O2S/Bi2O3 heterojunction self-powered photoelectrochemical sensors for sensitive detection of tetracycline

Materials & Design, Год журнала: 2024, Номер 239, С. 112781 - 112781

Опубликована: Фев. 23, 2024

The overuse of tetracycline, a broad-spectrum antibiotic, has posed severe threat to both the ecological environment and public safety. This paper presents one-step synthesis Bi2O2S/Bi2O3 heterojunctions utilizing simple environmentally friendly chemical method. These were subsequently employed in fabrication photoelectrochemical sensors tailored for tetracycline detection. Under optimal conditions, with bias voltage 0 V, sensor manifests exceptional self-powered capability, yielding photocurrent density approximately 270nA/cm2. detection sensitivity spans wide linear range from 0.1 pM 200 nM, accompanied by remarkably low limit 0.5 (S/N = 3). Furthermore, demonstrates commendable stability, rapid response characteristics, high selectivity, consistent reproducibility. findings not only offer novel opportunity advancing but also present fresh perspective realm development.

Язык: Английский

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Paper-based point of care diagnostics for cancer biomarkers

Sensors & Diagnostics, Год журнала: 2024, Номер 3(4), С. 504 - 535

Опубликована: Янв. 1, 2024

Advancements in assay design, detection techniques, signal transduction and enhancement strategies using smart nanomaterials.

Язык: Английский

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