Sensors and Actuators B Chemical, Год журнала: 2023, Номер 393, С. 134193 - 134193
Опубликована: Июнь 21, 2023
Язык: Английский
Sensors and Actuators B Chemical, Год журнала: 2023, Номер 393, С. 134193 - 134193
Опубликована: Июнь 21, 2023
Язык: Английский
Talanta, Год журнала: 2023, Номер 266, С. 125124 - 125124
Опубликована: Авг. 26, 2023
Язык: Английский
Процитировано
53Food Chemistry, Год журнала: 2024, Номер 458, С. 140330 - 140330
Опубликована: Июль 3, 2024
Язык: Английский
Процитировано
40Coordination Chemistry Reviews, Год журнала: 2024, Номер 508, С. 215761 - 215761
Опубликована: Март 12, 2024
Язык: Английский
Процитировано
25Environmental Technology & Innovation, Год журнала: 2024, Номер 34, С. 103625 - 103625
Опубликована: Апрель 4, 2024
Contaminants, such as nucleic acids or toxic small molecules, threaten both human health and ecosystems when they infiltrate the environment. The precise highly sensitive identification of contaminants holds paramount importance across diverse domains, including safeguarding food integrity, facilitating clinical diagnostics, monitoring environmental conditions. Traditional methodologies, encompassing spectroscopy, chromatography, sequencing, metagenomics, have conventionally served pivotal roles in detection processes. Nevertheless, these methods encountered recurring challenges related to sensitivity, specificity, portability. This review focuses on groundbreaking CRISPR/Cas12-based biosensors. These biosensors leverage incredible precision programmability CRISPR/Cas system recognize specific targets. Here, we comprehensively assess fundamental mechanisms that enable detection, ranging from guide RNA design collateral cleavage. versatility CRISPR/Cas12 becomes evident through their applications. applications encompass medical safety, monitoring. transition conventional ultimately represents a significant milestone contaminant detection. By incorporating molecular biology, nanotechnology, bioinformatics, potential reshape landscape water CRIPSR-Cas diagnostics is transformative technology paves way for safer healthier future environment life.
Язык: Английский
Процитировано
23Coordination Chemistry Reviews, Год журнала: 2024, Номер 510, С. 215799 - 215799
Опубликована: Апрель 1, 2024
Язык: Английский
Процитировано
22Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 159557 - 159557
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
3TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 166, С. 117198 - 117198
Опубликована: Июль 24, 2023
Язык: Английский
Процитировано
26Analytical Chemistry, Год журнала: 2024, Номер 96(3), С. 1232 - 1240
Опубликована: Янв. 2, 2024
The emergence of nanoenzymes has catalyzed the robust advancement lateral flow immunoassay (LFIA) in recent years. Among them, multifunctional nanocomposite enzymes with core–shell architectures are considered preferable for promoting sensing ability due to their good biocompatibility, precise control over size, and surface properties etc. Herein, we developed a dual-channel ensured (DFLIA) platform utilizing magnetic, colorimetric, catalytic enzyme (Fe3O4@TCPP@Pd) [TCPP, Tetrakis (4-carboxyphenyl) porphyrin] ultrasensitive highly accurate rapid detection Escherichia coli O157:H7 (E. O157:H7). Fe3O4@TCPP@Pd-mAb exhibits superior performance compared traditional AuNPs, including enhanced sensitivity an extended linear range, benefiting from its high brightness signal, strong magnetic separation ability, peroxidase activity (Vmax = 2.32 μM S1–). Moreover, Fe3O4@TCPP@Pd-labeled mAb probe exhibited exceptional stability affinity toward E. (with constant approximately 1.723 × 109 M–1), indicating potential efficient capture pathogen. Impressively, Fe3O4@TCPP@Pd-DFLIA achieved pre- postcatalytic naked-eye sensitivities 255 cfu/mL 77 cfu/mL, respectively, representing 41-fold improvement conventional AuNP-based LFIA also possessed specificity reproducibility [relative standard deviation (RSD) < 10%]. Additionally, established DFLIA satisfactory recoveries detecting pork milk samples, further validating reliability this immunoassays demonstrating utilization bioassays clinical diagnostics.
Язык: Английский
Процитировано
13Chemical Engineering Journal, Год журнала: 2024, Номер 486, С. 150279 - 150279
Опубликована: Март 9, 2024
Язык: Английский
Процитировано
13Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 161110 - 161110
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
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