Electrochemical Biosensors 3D Printed by Fused Deposition Modeling: Actualities, Trends, and Challenges

Biosensors, Год журнала: 2025, Номер 15(1), С. 57 - 57

Опубликована: Янв. 17, 2025

The technology of 3D printing, particularly fused deposition modeling (FDM) has revolutionized the development electrochemical biosensors, offering a versatile and cost-effective approach for clinical applications. This review explores integration FDM in fabricating biosensing platforms tailored diagnostics, emphasizing its role detecting various biomarkers viral pathogens. Advances printing materials, especially emergence bespoke conductive filaments, have allowed production highly customizable efficient biosensors. A detailed discussion focuses on design application these biosensors detection, highlighting their potential to improve diagnostic accuracy. Furthermore, addresses current trends, including push towards miniaturization multianalyte alongside challenges such as material optimization regulatory hurdles. By providing comprehensive overview, this work underscores transformative impact 3D-printed diagnostics while also identifying critical areas future research development.

Язык: Английский

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Electrochemical vs. Optical Biosensors for Point-of-Care Applications: A Critical Review

Chemosensors, Год журнала: 2023, Номер 11(10), С. 546 - 546

Опубликована: Окт. 21, 2023

Analytical chemistry applied to medical and diagnostic analysis has recently focused on the development of cost-effective biosensors able monitor health status or assess level specific biomarkers that can be indicative several diseases. The improvement technologies relating possibility non-invasive sampling biological fluids, as well sensors for detection analytical signals computational capabilities systems routinely employed in everyday life (e.g., smartphones, computers, etc.), makes complete integration self-standing devices more accessible. This review aims discuss have been proposed last five years focusing two principal detecting approaches, optical electrochemical, which quantifying different kinds target analytes reaching limits below clinical sample levels required. These principles point-of-care (POC) extensively reported literature, even limited examples found market are based these strategies. work will show latest innovations considering electrochemical with most commonly platforms POC applications such paper-based wearable implantable devices.

Язык: Английский

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Developing a nanomaterial-based 3D-printed platform: Application as a cancer aptasensor via detection of heat shock protein 90 (HSP90)

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 409, С. 135592 - 135592

Опубликована: Март 4, 2024

Язык: Английский

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Real-Time Visualization of Endogenous H₂O₂ Production in Mammalian Spheroids by Electrochemiluminescence

Chemical & Biomedical Imaging, Год журнала: 2025, Номер 3(5), С. 310 - 321

Опубликована: Март 10, 2025

Two-dimensional cell culture may be insufficient when it comes to understanding human disease. The redox behavior of complex, three-dimensional tissue is critical disease genesis and propagation. Unfortunately, few measurement tools are available for such models yield quantitative insight into how reactive oxygen species (ROS) form over time. Here, we demonstrate an imaging platform the real-time visualization H2O2 formation mammalian spheroids made noncancerous embryonic kidney cells (HEK-293) metastatic breast cancer (MCF-7 MDA-MB-231). We take advantage luminol electrochemiluminescence reaction on a transparent tin-doped indium oxide electrode. luminescence this as function [H2O2] linear (R2 = 0.98) with dynamic range between 0.5 μM 0.1 mM, limit detection 2.26 ± 0.58 μM. Our method allows observation ROS activity in growing days advance current techniques without need sacrifice sample postanalysis. Finally, use our procedure key pathways cancerous can up-regulated downregulated through addition common metabolic drugs, rotenone carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. results suggest that Warburg Effect studied single spheroids, drugs one implicate specific formation. expect diagnostic tool have wide applications propagation system more closely related tissue.

Язык: Английский

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3D printed thermoplastic sensors for electrochemical biosensing

Current Opinion in Electrochemistry, Год журнала: 2025, Номер unknown, С. 101699 - 101699

Опубликована: Апрель 1, 2025

Язык: Английский

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Machine learning assisted and smartphone integrated homogeneous electrochemiluminescence biosensor platform for sample to answer detection of various human metabolites

Biosensors and Bioelectronics, Год журнала: 2023, Номер 238, С. 115582 - 115582

Опубликована: Авг. 8, 2023

Язык: Английский

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The 3D Printing of Nanocomposites for Wearable Biosensors: Recent Advances, Challenges, and Prospects

Bioengineering, Год журнала: 2023, Номер 11(1), С. 32 - 32

Опубликована: Дек. 27, 2023

Notably, 3D-printed flexible and wearable biosensors have immense potential to interact with the human body noninvasively for real-time continuous health monitoring of physiological parameters. This paper comprehensively reviews progress in biosensors. The review also explores incorporation nanocomposites 3D printing A detailed analysis various processes fabricating is reported. Besides this, recent advances platforms such as sweat sensors, glucose electrocardiography electroencephalography tactile oximeters, tattoo respiratory sensors are discussed. Furthermore, challenges prospects associated presented. an invaluable resource engineers, researchers, healthcare clinicians, providing insights into advancements capabilities biosensor domain.

Язык: Английский

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A smartphone-based fluorescent biosensor with metal-organic framework biocomposites and cotton swabs for the rapid determination of tetrodotoxin in seafood

Analytica Chimica Acta, Год журнала: 2024, Номер 1311, С. 342738 - 342738

Опубликована: Май 17, 2024

Язык: Английский

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Deep Learning-Assisted Smartphone-Based Electrochemiluminescence Visual Monitoring Biosensor: A Fully Integrated Portable Platform

Micromachines, Год журнала: 2024, Номер 15(8), С. 1059 - 1059

Опубликована: Авг. 22, 2024

A novel, portable deep learning-assisted smartphone-based electrochemiluminescence (ECL) cost-effective (~10$) sensing platform was developed and used for selective detection of lactate. Low-cost, fast prototyping screen printing wax methods with paper-based substrate were to fabricate miniaturized single-pair electrode ECL platforms. The lab-made 3D-printed black box served as a reaction chamber. This integrated smartphone buck-boost converter, eliminating the need expensive CCD cameras, photomultiplier tubes, bulky power supplies. advancement makes this ideal point-of-care testing applications. Foremost, integration learning approach enhance not just accuracy sensors, but also expedite diagnostic procedure. models trained (3600 images) tested (900 using images obtained from experimentation. Herein, user convenience, an Android application graphical interface developed. app performs several tasks, which include capturing real-time images, cropping them, predicting concentration required bioanalytes through learning. device’s capability work in real environment by performing lactate sensing. fabricated device shows good liner range (from 50 µM 2000 µM) acceptable limit value 5.14 µM. Finally, various rigorous analyses, including stability, reproducibility, unknown sample analysis, conducted check durability stability. Therefore, becomes versatile applicable across domains harnessing cutting-edge technology integrating it smartphone.

Язык: Английский

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Confinement-enhanced electrochemiluminescence by Ru(dcbpy)32+-functionalized γ-CD-MOF@COF-LZU1 porous hybrid material as micro-reactor for CYFRA 21-1 detection

Talanta, Год журнала: 2024, Номер 273, С. 125959 - 125959

Опубликована: Март 26, 2024

Язык: Английский

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