Electrochemical Investigation of TLR4/MD-2-Immobilized Polyaniline and Hollow Polyaniline Nanofibers: Toward Real-Time Triaging of Gram-Negative Bacteria Responsible for Delayed Wound Healing DOI
Rahul Gangwar, Pravat Kumar Sahu, Karri Trinadha Rao

и другие.

IEEE Sensors Letters, Год журнала: 2023, Номер 7(12), С. 1 - 4

Опубликована: Окт. 19, 2023

Detecting gram −ve bacterial colonies is crucial in address-ing the clinical challenges associated with chronic wounds and delayed healing. These bacteria can exacerbate wound conditions, hindering natural healing potentially leading to infections. The electrochemical sensing platform presented this study serves as a valuable tool for healthcare professionals make timely targeted treatment decisions. Toward this, we developed cost-effective leveraging TLR4/MD-2 complex detect colonies. Our biosensors were meticulously fashioned using polyaniline (PANi) hollow PANi (HPANi) nanofibers. Notably, HPANi-based sensors, owing their distinctive structure, facilitated amplified responses under comparable experimental conditions compared PANi-based counterparts. designed demonstrated exceptional accuracy identifying Escherichia coli (gram −ve), showcasing theoretical detection limit of 0.215 CFU/mL remarkably improved 0.14 HPANi. sensors displayed outstanding selectivity bacteria, even amidst +ve fungi. Moreover, our remarkable sensitivity, yielding 3.04 ((ΔR/R)/CFU/mL)/cm 2 sensor, surpassing performance sensor at 1.98 .

Язык: Английский

ZnO Nanoflower-Based Electrochemical SARS-CoV-2 Molecular Biosensors with Improved Diagnostic Accuracy DOI

Ullas Pandey,

Partha Pratim Goswami, Shiv Govind Singh

и другие.

ACS Applied Nano Materials, Год журнала: 2024, Номер 7(1), С. 683 - 694

Опубликована: Янв. 4, 2024

Despite the promising performance of nanomaterial-based electrochemical detection techniques, product-level point-of-care sensor systems are still evolving. Unique approaches toward nanoscale tunability, enriched measurements, and data analysis therefore imperative. The recent outbreak coronavirus disease provides a suitable test bench this pursuit, attracting several innovative efforts from research community. Accordingly, work reports an ultrasensitive selective molecular biosensor for RT-PCR-confirmed hospital samples SARS-CoV-2 built on ZnO nanoflowers. unique experimental analytical methodologies drastically improve device efficiency, relying three conserved genomic sequences (E, N, ORF1ab genes). It involves multiple techniques like cyclic voltammetry, impedance spectroscopy, differential pulse voltammetry where normalized responses these used to create 1D, 2D, 3D points representing each sample clustering both by heuristic (2D separation plane) ML approach (logistic regression) accurate diagnosis. Enhancing features also enlarges Euclidean distances (ED) points, which raises efficiency 93.75%. As space allows (with nearly identical EDs) have different spatial orientations efficient clustering, were dichotomized into positive negative with 100% sensitivity, specificity. Furthermore, owing nanostructured transducer, viral load was successfully quantified in spiked control commendable sensitivity limits as low 14.137 fM (E-gene). device's fast response time (<45 min) justifies its potential on-field applicability.

Язык: Английский

Процитировано

5
One-step copper deposition-induced signal amplification for multiplex bacterial infection diagnosis on a lateral flow immunoassay device DOI

Yuh-Shiuan Chien,

Tsung‐Ting Tsai,

Jiahui Lin

и другие.

Biosensors and Bioelectronics, Год журнала: 2024, Номер 267, С. 116849 - 116849

Опубликована: Окт. 11, 2024

Язык: Английский

Процитировано

2
Silk-Polyurethane Composite based flexible electrochemical biosensing platform for pathogen detection DOI
Karri Trinadha Rao, Rahul Gangwar,

Aditya Bhagavathi

и другие.

Biosensors and Bioelectronics, Год журнала: 2024, Номер 271, С. 117024 - 117024

Опубликована: Дек. 2, 2024

Язык: Английский

Процитировано

2
Green-Synthesized Amino Carbons for Impedimetric Biosensing of E. coli O157:H7 DOI
Pravat Kumar Sahu, Rahul Gangwar,

Asha Ramesh

и другие.

ACS Infectious Diseases, Год журнала: 2024, Номер 10(5), С. 1644 - 1653

Опубликована: Апрель 11, 2024

This study describes the synthesis of amino-functionalized carbon nanoparticles derived from biopolymer chitosan using green and its application toward ultrasensitive electrochemical immunosensor highly virulent Escherichia coli O157:H7 (E. O157:H7). The inherent advantage high surface-to-volume ratio enhanced rate transfer kinetics is leveraged to push limit detection (LOD), without compromising on selectivity. prepared were systematically characterized by employing CO2-thermal programmed desorption (CO2-TPD), Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), ultraviolet–visible (UV–visible), transmission electron microscopy (TEM). estimated 0.74 CFU/mL a sensitivity 5.7 ((ΔRct/Rct)/(CFU/mL))/cm2 in impedance (EIS) affirm utility sensor. proposed biosensor displayed remarkable selectivity against interfering species, making it well suited for real-time applications. Moreover, chitosan-derived semiconducting shows excellent comparative analysis compared conducting amine-functionalized synthesized via chemical modification, demonstrating vast potential as an E.

Язык: Английский

Процитировано

1
Rapid assembly of mixed thiols for toll-like receptor-based electrochemical pathogen sensing DOI Creative Commons
Rajesh G. Pillai,

Khalid Azyat,

Nora W. C. Chan

и другие.

Analytical Methods, Год журнала: 2024, Номер 16(41), С. 7021 - 7032

Опубликована: Янв. 1, 2024

Herein, we describe a rapid and facile fabrication of electrochemical sensors utilizing two different toll-like receptor (TLR) proteins as biorecognition elements to detect bacterial pathogen associated molecular patterns (PAMPs).

Язык: Английский

Процитировано

1
Electrochemical Investigation of TLR4/MD-2-Immobilized Polyaniline and Hollow Polyaniline Nanofibers: Toward Real-Time Triaging of Gram-Negative Bacteria Responsible for Delayed Wound Healing DOI
Rahul Gangwar, Pravat Kumar Sahu, Karri Trinadha Rao

и другие.

IEEE Sensors Letters, Год журнала: 2023, Номер 7(12), С. 1 - 4

Опубликована: Окт. 19, 2023

Detecting gram −ve bacterial colonies is crucial in address-ing the clinical challenges associated with chronic wounds and delayed healing. These bacteria can exacerbate wound conditions, hindering natural healing potentially leading to infections. The electrochemical sensing platform presented this study serves as a valuable tool for healthcare professionals make timely targeted treatment decisions. Toward this, we developed cost-effective leveraging TLR4/MD-2 complex detect colonies. Our biosensors were meticulously fashioned using polyaniline (PANi) hollow PANi (HPANi) nanofibers. Notably, HPANi-based sensors, owing their distinctive structure, facilitated amplified responses under comparable experimental conditions compared PANi-based counterparts. designed demonstrated exceptional accuracy identifying Escherichia coli (gram −ve), showcasing theoretical detection limit of 0.215 CFU/mL remarkably improved 0.14 HPANi. sensors displayed outstanding selectivity bacteria, even amidst +ve fungi. Moreover, our remarkable sensitivity, yielding 3.04 ((ΔR/R)/CFU/mL)/cm 2 sensor, surpassing performance sensor at 1.98 .

Язык: Английский

Процитировано

1