Detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi-distributed FBG-assisted interferometric probes

Sensing and Bio-Sensing Research, Год журнала: 2024, Номер 46, С. 100699 - 100699

Опубликована: Окт. 23, 2024

Язык: Английский

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Acoustic-assisted centrifugal microfluidics for particle/cell separation

Chemical Engineering and Processing - Process Intensification, Год журнала: 2024, Номер 201, С. 109803 - 109803

Опубликована: Апрель 29, 2024

Язык: Английский

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Electrochemical Sensors: Types, Applications, and the Novel Impacts of Vibration and Fluid Flow for Microfluidic Integration

Biosensors and Bioelectronics, Год журнала: 2025, Номер 272, С. 117099 - 117099

Опубликована: Янв. 2, 2025

Язык: Английский

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Portable, Quantitative, Real-time Isothermal Nucleic Acid Amplification Test Using Microfluidic Device-Coupled UV-LED Photodiode Detector

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117194 - 117194

Опубликована: Янв. 1, 2025

Язык: Английский

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Visual Detection of Canine Monocytic Ehrlichiosis Using Polymerase Chain Reaction-Based Lateral Flow Biosensors

Animals, Год журнала: 2025, Номер 15(5), С. 740 - 740

Опубликована: Март 5, 2025

A conventional PCR (cPCR) remains an effective molecular technique for the diagnosis of canine monocytic ehrlichiosis. However, agarose gel electrophoresis requires additional time after thermal cycling. In present study, we developed a PCR-based lateral flow biosensor (PCR-LFB) to detect Ehrlichia canis (E. canis). Lateral strips allow simple and rapid detection products provide alternative electrophoresis. The sensitivity, specificity, limit PCR-LFB were compared those TaqMan probe-based real-time PCRs (qPCRs). was performed with 5' 6-FITC biotin-labeled primers specific E. canis, targeting dsb gene. assay 10-6 target DNA sequence in 10-fold dilution recombinant plasmid, which is 10 times lower than that qPCR. Among confirmed qPCR results 30 dog samples, false-positive not detected by PCR-LFB. Compared qPCR, sensitivity specificity 63.6% (95% CI; 42.9-80.2%) 100% 67.5-100%), respectively. Kappa value moderate agreement (κ = 0.483). Perfect 1) observed between cPCR Lower cost shorter consumption demonstrated using

Язык: Английский

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Airborne Influenza Virus Surveillance Platform Using Paper-Based Immunosensors and a Growth-Based Virus Aerosol Concentrator

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Март 30, 2025

The measurement of respiratory viruses in indoor air is critical for effectively preventing the spread diseases. This typically accomplished by counting nucleic acids or plaques air-sampled viruses. Herein, we present a growth-based airborne virus surveillance (G-AVS) platform based on paper-based electrochemical immunosensors targeting hemagglutinin (HA) and nucleoprotein (NP), water-condensation sampling quantitative influenza measurements, compared with RT-qPCR, demonstrated consistency between two. In measurements conducted an elementary school using G-AVS, 23% (4/17) samples were positive, concentrations ranging from 1.7 × 104 to 1.6 106 gene copies/m3, while losses HA relative NP 48–75% at humidity 27.0–36.8% 60 min sampling, similar infectivities reported literature. has potential rapid cost-effective measurement.

Язык: Английский

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Pico-Scale Digital PCR on a Super-Hydrophilic Microarray Chip for Multi-Target Detection

Micromachines, Год журнала: 2025, Номер 16(4), С. 407 - 407

Опубликована: Март 30, 2025

The technology of digital polymerase chain reaction (dPCR) is rapidly evolving, yet current devices often suffer from bulkiness and cumbersome sample-loading procedures. Moreover, challenges such as droplet merging partition size limitations impede efficiency. In this study, we present a super-hydrophilic microarray chip specifically designed for dPCR, featuring streamlined loading methods compatible with micro-electro-mechanical systems (MEMS) technology. Utilizing hydrodynamic principles, our platform enables the formation uniform array 120-pL independent units within closed channel. setup allows rapid reactions facilitated by an efficient thermal cycler real-time imaging. We achieved absolute quantitative detection hepatitis B virus (HBV) plasmids at varying concentrations, alongside multiple targets, including cancer mutation gene fragments reference genes. This work highlights chip’s versatility potential applications in point-of-care testing (POCT) diagnostics.

Язык: Английский

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CLIA immunoassay as an alternative and accurate method to detect Sars-Cov-2 antigen compared to ELISA

Diagnostic Microbiology and Infectious Disease, Год журнала: 2025, Номер 112(4), С. 116828 - 116828

Опубликована: Апрель 5, 2025

Язык: Английский

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Low-cost optical sensors in electrified lab-on-a-disc platforms: liquid-phase boundary detection and automated diagnostics

Microsystems & Nanoengineering, Год журнала: 2025, Номер 11(1)

Опубликована: Апрель 7, 2025

Abstract Centrifugal microfluidic platforms are highly regarded for their potential in multiplexing and automation, as well wide range of applications, especially separating blood plasma manipulating two-phase flows. However, the need to use stroboscopes or high-speed cameras monitoring these tasks hinders extensive research commercial settings. In this study, we introduce an innovative cost-effective strategy using array light-dependent resistors (LDRs) optical sensors devices, particularly centrifugal platforms. While LDRs attractive photodetectors, bulky size frequently restricts ability provide high-resolution detection systems. Here, specific waveguides direct light beams from narrow apertures onto surface LDRs. We integrated into electrified Lab-on-a-Disc (eLOD) with wireless connectivity smartphones laptops. This enables many such droplet/particle counting velocity measurement, concentration analysis, fluidic interface multiphase flows, real-time sample volume on platforms, separation alternative costly stroboscope microscopes, imaging. used numerical simulations evaluate various fluids scenarios, which include rotation speeds up 50 rad/s a droplet sizes. For testbed, developed eLOD device analyze red cell (RBC) deformability improve automated sickle anemia by differences RBC during centrifugation sensors’ signals. addition anemia, has facilitate low-cost other medical conditions characterized altered deformability, thalassemia, malaria, diabetes.

Язык: Английский

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Optimizing microfluidic chip for rapid SARS-CoV-2 detection using Taguchi method and artificial neural network PSO

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 23, 2025

Abstract Microfluidic biosensors offer a promising solution for real-time analysis of coronaviruses with minimal sample volumes. This study optimizes biochip the rapid detection SARS-CoV-2 using Taguchi orthogonal table L 9 (3 4 ), which comprises nine groups experiments varying four key parameters: Reynolds number (Re), Damköhler (Da), Schmidt (Sc), and dimensionless position reaction surface (X). Signal-to-noise (S/N) ratios variance (ANOVA) are employed to determine optimal parameters assess their impact on binding kinetics response time device. These obtained correspond Re = 4.10 -2 , Da 1000, Sc 10 5 X 1. Additionally, results highlight as most influential factor, accounting 91%, while has effect 0.3%. Furthermore, an artificial neural network optimization technique, specifically particle swarm (PSO), was utilized predict biosensor performance. Derived from Full 81 ) design experiment, PSO model demonstrates its effectiveness compared conventional multi-layer perception (MLP) model, thus underlining potential in this innovative context.

Язык: Английский

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