Journal of Innovative Optical Health Sciences,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 28, 2024
Programmed
cell
death
(PCD)
plays
a
crucial
role
in
the
biological
processes
of
living
organisms
and
occurs
various
forms,
such
as
apoptosis,
necroptosis
ferroptosis.
However,
traditional
methods
for
PCD
analysis
are
time-consuming
complex.
In
this
paper,
we
propose
facile
surface-enhanced
Raman
spectroscopy
(SERS)-based
strategy
real-time
three
patterns
utilizing
black
phosphorus–gold
nanoparticles
(BP–Au
NPs)
ultrasensitive
unlabeled
probe.
BP–Au
NPs,
which
possess
excellent
biocompatibility,
capable
detecting
dye
molecules
at
concentrations
low
[Formula:
see
text][Formula:
text]M
remain
stable
least
one
week
different
physiological
environments.
view
this,
NPs-based
SERS
technique
can
distinguish
tiny
differences
molecular
fingerprints
cancer
cells
undergoing
(apoptosis,
ferroptosis)
triggered
by
doxorubicin,
shikonin
erastin,
respectively.
We
also
have
monitoring
intracellular
events
during
PCD,
spy
fluctuations
some
typical
bands
assigned
to
protein,
DNA
lipid,
revealing
unique
phenotypic
characteristics
each
pattern.
This
provides
detailed
comprehensive
mechanisms
drug-induced
level.
Nano TransMed,
Год журнала:
2024,
Номер
3, С. 100032 - 100032
Опубликована: Янв. 29, 2024
Nanozymes
exhibit
immense
potential
and
promising
prospects
in
the
realm
of
diagnostic
imaging
tumor
therapy,
owing
to
their
inherent
physicochemical
properties
enzymatic
activities.
With
further
development
application
nanotechnology,
nanozymes
are
expected
become
an
important
tool
field
medicine,
providing
new
solutions
for
disease
diagnosis
treatment.
This
review
provides
overview
recent
studies
over
past
five
years
on
capable
responding
microenvironment
(TME)
multimodal
imaging,
with
a
focus
hypoxia,
acid
pH,
excess
GSH
ROS.
In
addition,
we
present
current
status
several
common
related
contrast
agents,
such
as
MRI/CT,
MRI/PET,
MRI/SPECT,
PET/CT.
Moreover,
some
thoughts
challenges,
future
development,
clinical
translation
microenvironment-responsive
shared.
aims
provide
up-to-date
theranostic
nanomedicine
reference
those
who
fields,
promote
progress
this
interdisciplinary
subject,
accelerate
translation.
Abstract
Rapid
and
visual
detection
of
SARS‐CoV‐2
variants
is
vital
for
timely
assessment
variant
transmission
in
resource‐limited
settings.
Here,
a
closed‐tube,
two‐stage,
mixed‐dye‐based
isothermal
amplification
method
with
ribonuclease‐cleavable
enhanced
probes
(REP),
termed
REP‐TMAP,
dual‐visualization
including
JN.1,
BA.2,
BA.4/5,
Delta
introduced.
The
first
stage
REP‐TMAP
reverse
transcription
recombinase
polymerase
the
second
synergistically
mediated
by
REP
mixed
dyes
cresol
red
hydroxy
naphthol
blue.
In
reaction,
color
change
under
ambient
light
indicates
infection,
while
fluorescence
blue
excitation
specifies
type.
On
detecting
transcribed
RNA
spike
gene,
this
assay
rapid
(within
40
min),
highly
sensitive
(10–200
copies
per
reaction),
specific
(identification
single‐base
mutations).
Furthermore,
has
been
clinically
validated
to
accurately
detect
BA.4/5
from
102
human
oropharyngeal
swabs.
proposed
therefore
holds
great
potentials
provide
rapid,
dual‐visualization,
sensitive,
specific,
point‐of‐care
beyond.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(28), С. 36106 - 36116
Опубликована: Июль 2, 2024
Accurate
detection
of
heterogeneous
circulating
tumor
cells
(CTCs)
is
critical
as
they
can
make
more
aggressive,
drug-resistant,
and
metastasizing.
Although
the
leukocyte
membrane
coating
strategy
promising
in
meeting
challenge
detecting
CTCs
due
to
its
inherent
antiadhesive
properties,
it
still
limited
by
reduction
or
loss
expression
known
markers.
Bioorthogonal
glycol-metabolic
engineering
expected
break
down
this
barrier
feeding
with
sugar
derivatives
a
unique
functional
group
establish
artificial
targets
on
surface
cells.
Herein,
an
engineered
biomimetic
colorimetric
sensor
was
accordingly
fabricated
for
high-efficient
CTCs.
Compared
conventional
coating,
could
covalently
bound
models
fed
Ac4ManNAz
vitro
through
synergy
bioorthogonal
chemistry
metabolic
glycoengineering,
ignoring
phenotypic
changes
Meanwhile,
sandwich
structure
composed
layer/CTCs/MoS2
nanosheet
formed
visual
HeLa
low
10
mL–1.
Overall,
approach
overcome
dependence
cell
technology
specific
phenotypes
provide
new
viewpoint
highly
efficiently
detect
Microorganisms,
Год журнала:
2024,
Номер
12(7), С. 1400 - 1400
Опубликована: Июль 11, 2024
The
aim
of
the
study
was
to
establish
a
live/dead
qPCR
with
propidium
monoazide
(PMA)
that
can
quantitatively
differentiate
between
viable/non-viable
microorganisms
in
dental
hard
tissues.
Human
premolars
(n
=
88)
were
prepared
nickel–titanium
instruments
and
incubated
E.
faecalis
(21
d).
Subsequently,
bacteria
half
teeth
devitalized
by
heat
inactivation
(100
°C,
2
h).
following
parameters
tested:
PMA
concentrations
at
0
µmol
(control),
50
µmol,
100
200
µmol;
incubation
times
30
min
60
min,
blue
light
treatment
for
min.
ground
using
cryomill
bacterial
DNA
quantified
qPCR,
ANOVA,
p
0.05.
control
group
detected
similar
number
avital
9.94
×
106
vital
1.61
107
cells.
use
inhibited
amplification
from
non-viable
cells
during
qPCR.
As
result,
best
detection
achieved
parameters:
(concentration,
time,
treatment)
200-30-30;
5.53
104
(avital)
1.21
100.7
(vital).
method
is
suitable
differentiation
quantification
dentin,
as
well
evaluate
effectiveness
different
preparation
procedures
antimicrobial
irrigants
other
biological
substances.
