Real-time SERS analysis of programmed cell death patterns based on black Phosphorus–gold nanoparticles DOI Creative Commons
Jieyi Chen,

Binggang Ye,

Ranran Fan

и другие.

Journal of Innovative Optical Health Sciences, Год журнала: 2024, Номер unknown

Опубликована: Авг. 28, 2024

Programmed cell death (PCD) plays a crucial role in the biological processes of living organisms and occurs various forms, such as apoptosis, necroptosis ferroptosis. However, traditional methods for PCD analysis are time-consuming complex. In this paper, we propose facile surface-enhanced Raman spectroscopy (SERS)-based strategy real-time three patterns utilizing black phosphorus–gold nanoparticles (BP–Au NPs) ultrasensitive unlabeled probe. BP–Au NPs, which possess excellent biocompatibility, capable detecting dye molecules at concentrations low [Formula: see text][Formula: text]M remain stable least one week different physiological environments. view this, NPs-based SERS technique can distinguish tiny differences molecular fingerprints cancer cells undergoing (apoptosis, ferroptosis) triggered by doxorubicin, shikonin erastin, respectively. We also have monitoring intracellular events during PCD, spy fluctuations some typical bands assigned to protein, DNA lipid, revealing unique phenotypic characteristics each pattern. This provides detailed comprehensive mechanisms drug-induced level.

Язык: Английский

Tumor microenvironment responsive nanozymes for multimodal imaging of tumors DOI Creative Commons
Heng Wang, Wenrui Ouyang, Hongxing Liu

и другие.

Nano TransMed, Год журнала: 2024, Номер 3, С. 100032 - 100032

Опубликована: Янв. 29, 2024

Nanozymes exhibit immense potential and promising prospects in the realm of diagnostic imaging tumor therapy, owing to their inherent physicochemical properties enzymatic activities. With further development application nanotechnology, nanozymes are expected become an important tool field medicine, providing new solutions for disease diagnosis treatment. This review provides overview recent studies over past five years on capable responding microenvironment (TME) multimodal imaging, with a focus hypoxia, acid pH, excess GSH ROS. In addition, we present current status several common related contrast agents, such as MRI/CT, MRI/PET, MRI/SPECT, PET/CT. Moreover, some thoughts challenges, future development, clinical translation microenvironment-responsive shared. aims provide up-to-date theranostic nanomedicine reference those who fields, promote progress this interdisciplinary subject, accelerate translation.

Язык: Английский

Процитировано

22

An insight into the dual role of MoS2-based nanocarriers in anticancer drug delivery and therapy DOI
Sandip Ghosh, Jui‐Yang Lai

Acta Biomaterialia, Год журнала: 2024, Номер 179, С. 36 - 60

Опубликована: Март 27, 2024

Язык: Английский

Процитировано

19

Sustainable green synthesis of silver nanoparticles for safer biomedical application DOI
Mohammed Ali Dheyab, Azlan Abdul Aziz,

Shaymaa Hussein Nowfal

и другие.

Journal of environmental chemical engineering, Год журнала: 2025, Номер unknown, С. 115998 - 115998

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

3

Engineering and optimization of the SPR device ZnO/Ag/WO3/Ni/2D-Nanomaterials highly sensitive for biomedical processing and detection DOI
Mohamed El barghouti,

Fatima Houari,

Abdellatif Akjouj

и другие.

Optical Materials, Год журнала: 2024, Номер 149, С. 115019 - 115019

Опубликована: Фев. 5, 2024

Язык: Английский

Процитировано

16

The advancement of molybdenum disulfide quantum dots nanoparticles as nanocarrier for drug delivery systems: Cutting-edge in dual therapeutic roles DOI

MohammadHossein Karami,

Behzad Aghabarari

Journal of Molecular Structure, Год журнала: 2024, Номер 1318, С. 139149 - 139149

Опубликована: Июнь 27, 2024

Язык: Английский

Процитировано

6

Versatile Self-Assembled Near-Infrared SERS Nanoprobes For Multidrug-Resistant Bacterial Infection-Specific Surveillance and Therapy DOI
Qian Gao,

Ruocan Liu,

Yundi Wu

и другие.

Acta Biomaterialia, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

5

Nitrogen-doped MoS2 QDs as fluorescent probes for sensitive detection of curcumin and cell imaging DOI
Jia Ge,

Zhiyao Zhai,

Yuru Chen

и другие.

Analytica Chimica Acta, Год журнала: 2024, Номер 1325, С. 343090 - 343090

Опубликована: Авг. 10, 2024

Язык: Английский

Процитировано

4

Two‐Stage Mixed‐Dye‐Based Isothermal Amplification with Ribonuclease‐Cleavable Enhanced Probes for Dual‐Visualization Detection of SARS‐CoV‐2 Variants of Interest DOI
Xiong Ding, Yaru Wang,

Yuxin Gui

и другие.

Advanced Science, Год журнала: 2024, Номер 11(29)

Опубликована: Июнь 3, 2024

Abstract Rapid and visual detection of SARS‐CoV‐2 variants is vital for timely assessment variant transmission in resource‐limited settings. Here, a closed‐tube, two‐stage, mixed‐dye‐based isothermal amplification method with ribonuclease‐cleavable enhanced probes (REP), termed REP‐TMAP, dual‐visualization including JN.1, BA.2, BA.4/5, Delta introduced. The first stage REP‐TMAP reverse transcription recombinase polymerase the second synergistically mediated by REP mixed dyes cresol red hydroxy naphthol blue. In reaction, color change under ambient light indicates infection, while fluorescence blue excitation specifies type. On detecting transcribed RNA spike gene, this assay rapid (within 40 min), highly sensitive (10–200 copies per reaction), specific (identification single‐base mutations). Furthermore, has been clinically validated to accurately detect BA.4/5 from 102 human oropharyngeal swabs. proposed therefore holds great potentials provide rapid, dual‐visualization, sensitive, specific, point‐of‐care beyond.

Язык: Английский

Процитировано

3

Engineered Leukocyte Biomimetic Colorimetric Sensor Enables High-Efficient Detection of Tumor Cells Based on Bioorthogonal Chemistry DOI
Min Li,

Lanlan Jia,

Aihong Zhu

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(28), С. 36106 - 36116

Опубликована: Июль 2, 2024

Accurate detection of heterogeneous circulating tumor cells (CTCs) is critical as they can make more aggressive, drug-resistant, and metastasizing. Although the leukocyte membrane coating strategy promising in meeting challenge detecting CTCs due to its inherent antiadhesive properties, it still limited by reduction or loss expression known markers. Bioorthogonal glycol-metabolic engineering expected break down this barrier feeding with sugar derivatives a unique functional group establish artificial targets on surface cells. Herein, an engineered biomimetic colorimetric sensor was accordingly fabricated for high-efficient CTCs. Compared conventional coating, could covalently bound models fed Ac4ManNAz vitro through synergy bioorthogonal chemistry metabolic glycoengineering, ignoring phenotypic changes Meanwhile, sandwich structure composed layer/CTCs/MoS2 nanosheet formed visual HeLa low 10 mL–1. Overall, approach overcome dependence cell technology specific phenotypes provide new viewpoint highly efficiently detect

Язык: Английский

Процитировано

3

Establishment of a Protocol for Viability qPCR in Dental Hard Tissues DOI Creative Commons
Torsten Sterzenbach,

Vanessa Neumann,

Evelyn Trips

и другие.

Microorganisms, Год журнала: 2024, Номер 12(7), С. 1400 - 1400

Опубликована: Июль 11, 2024

The aim of the study was to establish a live/dead qPCR with propidium monoazide (PMA) that can quantitatively differentiate between viable/non-viable microorganisms in dental hard tissues. Human premolars (n = 88) were prepared nickel–titanium instruments and incubated E. faecalis (21 d). Subsequently, bacteria half teeth devitalized by heat inactivation (100 °C, 2 h). following parameters tested: PMA concentrations at 0 µmol (control), 50 µmol, 100 200 µmol; incubation times 30 min 60 min, blue light treatment for min. ground using cryomill bacterial DNA quantified qPCR, ANOVA, p 0.05. control group detected similar number avital 9.94 × 106 vital 1.61 107 cells. use inhibited amplification from non-viable cells during qPCR. As result, best detection achieved parameters: (concentration, time, treatment) 200-30-30; 5.53 104 (avital) 1.21 100.7 (vital). method is suitable differentiation quantification dentin, as well evaluate effectiveness different preparation procedures antimicrobial irrigants other biological substances.

Язык: Английский

Процитировано

3