Journal of Innovative Optical Health Sciences,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 28, 2024
Programmed
cell
death
(PCD)
plays
a
crucial
role
in
the
biological
processes
of
living
organisms
and
occurs
various
forms,
such
as
apoptosis,
necroptosis
ferroptosis.
However,
traditional
methods
for
PCD
analysis
are
time-consuming
complex.
In
this
paper,
we
propose
facile
surface-enhanced
Raman
spectroscopy
(SERS)-based
strategy
real-time
three
patterns
utilizing
black
phosphorus–gold
nanoparticles
(BP–Au
NPs)
ultrasensitive
unlabeled
probe.
BP–Au
NPs,
which
possess
excellent
biocompatibility,
capable
detecting
dye
molecules
at
concentrations
low
[Formula:
see
text][Formula:
text]M
remain
stable
least
one
week
different
physiological
environments.
view
this,
NPs-based
SERS
technique
can
distinguish
tiny
differences
molecular
fingerprints
cancer
cells
undergoing
(apoptosis,
ferroptosis)
triggered
by
doxorubicin,
shikonin
erastin,
respectively.
We
also
have
monitoring
intracellular
events
during
PCD,
spy
fluctuations
some
typical
bands
assigned
to
protein,
DNA
lipid,
revealing
unique
phenotypic
characteristics
each
pattern.
This
provides
detailed
comprehensive
mechanisms
drug-induced
level.
Microorganisms,
Год журнала:
2024,
Номер
12(7), С. 1400 - 1400
Опубликована: Июль 11, 2024
The
aim
of
the
study
was
to
establish
a
live/dead
qPCR
with
propidium
monoazide
(PMA)
that
can
quantitatively
differentiate
between
viable/non-viable
microorganisms
in
dental
hard
tissues.
Human
premolars
(n
=
88)
were
prepared
nickel–titanium
instruments
and
incubated
E.
faecalis
(21
d).
Subsequently,
bacteria
half
teeth
devitalized
by
heat
inactivation
(100
°C,
2
h).
following
parameters
tested:
PMA
concentrations
at
0
µmol
(control),
50
µmol,
100
200
µmol;
incubation
times
30
min
60
min,
blue
light
treatment
for
min.
ground
using
cryomill
bacterial
DNA
quantified
qPCR,
ANOVA,
p
0.05.
control
group
detected
similar
number
avital
9.94
×
106
vital
1.61
107
cells.
use
inhibited
amplification
from
non-viable
cells
during
qPCR.
As
result,
best
detection
achieved
parameters:
(concentration,
time,
treatment)
200-30-30;
5.53
104
(avital)
1.21
100.7
(vital).
method
is
suitable
differentiation
quantification
dentin,
as
well
evaluate
effectiveness
different
preparation
procedures
antimicrobial
irrigants
other
biological
substances.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Авг. 28, 2024
Recent
advancements
in
cancer
research
have
led
to
the
generation
of
innovative
nanomaterials
for
improved
diagnostic
and
therapeutic
strategies.
Despite
proven
potential
two-dimensional
(2D)
molybdenum
disulfide
(MoS
Journal of Applied Toxicology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 29, 2024
Abstract
The
success
of
graphene
oxides
has
gained
extensive
research
interests
in
developing
novel
2D
nanomaterials
(NMs).
WS
2
nanosheets
(NSs)
are
transition
metal‐based
NMs,
but
their
toxicity
is
unclear.
In
this
study,
we
investigated
the
oral
NSs
to
mouse
intestines.
Male
mice
were
administrated
with
vehicles,
1,
10,
or
100
mg/kg
via
intragastric
route,
once
a
day,
for
5
days.
results
indicate
that
did
not
induce
pathological
ultrastructural
changes
There
minimal
trace
elements
exposure
W
accumulation,
and
only
Co
levels
dose‐dependently
increased.
Lipid
droplets
observed
all
groups
mice,
lipidomics
data
significantly
decreased
four
lipid
species,
belonging
phosphatidylcholine
(PC).
proteins
regulating
autophagic
lipolysis,
namely,
LC3,
lysosomal
associated
membrane
protein
(LAMP2)
perilipin
(PLIN2),
increased,
it
was
statistically
different
LC3.
study
suggest
repeated
induced
influences
on
injury,
element
balance,
autophagy,
profiles
intestines,
indicating
relatively
high
biocompatibility
intestine
route.
Sensors,
Год журнала:
2024,
Номер
24(19), С. 6458 - 6458
Опубликована: Окт. 6, 2024
Developing
powerful
immunoassays
for
sensitive
and
real-time
detection
of
targets
has
always
been
a
challenging
task.
Due
to
their
advantages
direct
readout,
controllable
sensing,
low
background
interference,
photothermal
have
become
type
new
technology
that
can
be
used
various
applications
such
as
disease
diagnosis,
environmental
monitoring,
food
safety.
By
modification
with
antibodies,
materials
induce
temperature
changes
by
converting
light
energy
into
heat,
thereby
reporting
specific
target
recognition
events.
This
article
reviews
the
design
application
based
on
different
materials,
including
noble
metal
nanomaterials,
carbon-based
two-dimensional
oxide
sulfide
Prussian
blue
nanoparticles,
small
organic
molecules,
polymers,
etc.
It
pays
special
attention
role
working
principle
immunoassays.
Additionally,
challenges
prospects
future
development
are
briefly
discussed.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(50), С. 69798 - 69806
Опубликована: Дек. 4, 2024
Detection
of
Helicobacter
pylori
is
essential
for
the
prevention
gastric
cancer.
By
detecting
metabolized
NH3,
it
was
able
to
noninvasively
reveal
state
H.
pylori;
however,
still
a
challenge
since
NH3
concentration
much
lower
conventional
gas
sensors.
Herein,
we
developed
MoS2-based
sensor
continuous,
real-time
monitoring
growth.
The
atomic
thin
layer
and
all-exposed
surface
MoS2
facilitate
adsorption
charge
transferring.
A
high-response
prepared
by
decoration
depositing
metal
nanoparticles.
Fe-decorated
outperformed
with
high
response
40.9%
5.7
ppm
at
25
±
2
°C,
low
LOD
(6.2
ppb),
long-term
stability
12.5%
after
5
months.
applied
detection
in
situ
its
92
h
growth
cycle.
release
curve
exponential
phase
during
continuously
monitored,
quantified.
maximum
specific
rate
0.195
0.005
h–1,
which
well-consistent
nature
This
study
opens
up
technological
roadmap
noninvasive
future.
Journal of Innovative Optical Health Sciences,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 28, 2024
Programmed
cell
death
(PCD)
plays
a
crucial
role
in
the
biological
processes
of
living
organisms
and
occurs
various
forms,
such
as
apoptosis,
necroptosis
ferroptosis.
However,
traditional
methods
for
PCD
analysis
are
time-consuming
complex.
In
this
paper,
we
propose
facile
surface-enhanced
Raman
spectroscopy
(SERS)-based
strategy
real-time
three
patterns
utilizing
black
phosphorus–gold
nanoparticles
(BP–Au
NPs)
ultrasensitive
unlabeled
probe.
BP–Au
NPs,
which
possess
excellent
biocompatibility,
capable
detecting
dye
molecules
at
concentrations
low
[Formula:
see
text][Formula:
text]M
remain
stable
least
one
week
different
physiological
environments.
view
this,
NPs-based
SERS
technique
can
distinguish
tiny
differences
molecular
fingerprints
cancer
cells
undergoing
(apoptosis,
ferroptosis)
triggered
by
doxorubicin,
shikonin
erastin,
respectively.
We
also
have
monitoring
intracellular
events
during
PCD,
spy
fluctuations
some
typical
bands
assigned
to
protein,
DNA
lipid,
revealing
unique
phenotypic
characteristics
each
pattern.
This
provides
detailed
comprehensive
mechanisms
drug-induced
level.
