Simple, Rapid and Enzyme-Free Assay for Potentiometric Determination of L-Cysteine Based on Meso-2,3-Dimercaptosuccinic Acid Self-Assembled Gold Electrode DOI

Hong-Shu Xiao,

Bowen Wu, Bin Li

и другие.

Опубликована: Янв. 1, 2024

As a sulfur containing α-amino acid in nature, L-cysteine (L-Cys) plays crucial role variety of metabolic pathways with many important physiological functions. However, it is still great challenge to construct simple and rapid approach for L-Cys up now. In this paper, simple, enzyme-free method was developed potentiometric determination by using meso-2, 3-dimercaptosuccinic (DMSA) self-assembled monolayer on gold electrode (Au/DMSA). The surface morphology property membrane without combined were characterized means scanning electron microscopy, X-ray photoelectron spectroscopy, electrochemical impedance cyclic voltammetry, respectively. recognition mechanism may be attributed formation hydrogen bonds between DMSA the target molecule L-Cys, producing double electric layer at sensing interface, that potential decreases increasing concentration L-Cys. assay results showed good linear response Tris-HCl buffer solution (pH=7.0) obtained range 1.0×10-8 1.0×10-3 mol/L, slope -57.00±0.63 mV/-pc (25ºC) detection limit 7.9×10-9 mol/L. possessed fast time (45s), reproducibility, strong anti-interference performance. Moreover, can well applied pig serums recovery rate 94.3~106.3%, indicating promising application prospect.

Язык: Английский

Rapid Glutathione Analysis with SERS Microneedles for Deep Glioblastoma Tissue Differentiation DOI
Min Zhang, Yongping You, Hang Zhang

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(25), С. 10200 - 10209

Опубликована: Июнь 13, 2024

Rapid tissue differentiation at the molecular level is a prerequisite for precise surgical resection, which of special value treatment malignant tumors, such as glioblastoma (GBM). Herein, SERS-active microneedle prepared by modifying glutathione (GSH)-responsive molecules, 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), on surface Au@Ag substrates distinction different GBM tissues. Since Raman signals DTNB@Au@Ag can be collected both portable and benchtop spectrometers, distribution GSH in tissues centimeter scale displayed through spectroscopy imaging, entire analysis process accomplished within 12 min. Accordingly, vivo brain orthotopic xenograft mice ex patients are accurately differentiated with microneedle, results well consistent staining postoperative pathological reports. In addition, outline tumor, peritumoral, normal indicated least 56 days. Considering that tumor quickly discriminated without restriction depth, promising to powerful intraoperative diagnostic tool surgery navigation.

Язык: Английский

Процитировано

5
Based on Fe and Ni prepared organic colloidal materials as efficient oxide nanozymes for chemiluminescence detection of GSH and Hg(II) ions DOI

Xiluan Yan,

Shiyun Cheng,

Yipi Xiao

и другие.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2024, Номер 321, С. 124696 - 124696

Опубликована: Июнь 21, 2024

Язык: Английский

Процитировано

3
Simple, rapid and enzyme-free assay for potentiometric determination of L-cysteine based on meso-2,3-dimercaptosuccinic acid self-assembled gold electrode DOI
Yuanqing Wu,

Hong-Shu Xiao,

Yu‐Yu Peng

и другие.

Microchemical Journal, Год журнала: 2024, Номер 206, С. 111548 - 111548

Опубликована: Авг. 31, 2024

Язык: Английский

Процитировано

3
A copper response fluorescent probe for selective detection of GSH based on Nitrogen-doped carbon dots DOI

Huanrong Zuo,

Xingyu Hou, Na Sun

и другие.

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113642 - 113642

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
A near-infrared fluorescent probe selectively recognizing cysteine to release H2S and its applications DOI
Xuan Wang,

Nianwei Wang,

Yikun Ren

и другие.

The Analyst, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Cysteine, a pivotal biothiol vital for human health, exhibits strong associations with various diseases, yet its concentration assessment is often confounded by structurally and functionally similar biothiols. Hydrogen sulfide (H2S), an endogenous gasotransmitter therapeutic potential, faces challenges in achieving pharmacological effects due to the unpredictable presence of inorganic H2S donors. To address these issues, we developed TMN-ONCS, near-infrared fluorescent probe that integrates p-tolyl isothiocyanate dicyanoisophorone fluorophore through self-immolative spacer. TMN-ONCS demonstrates high selectivity analytical performance cysteine, avoids generation multiple fluorophores seen conventional probes, functions as cysteine-activated donor concurrent visualization. Furthermore, it mitigates lipopolysaccharide-induced inflammation RAW 264.7 cells, highlighting potential treating inflammation.

Язык: Английский

Процитировано

0
Homocysteine stimulates orbital fibroblast proliferation and migration in Graves' orbitopathy via activating Akt signaling pathway DOI Creative Commons
Weili Yang,

Xinyu Xu,

Rongrong Xie

и другие.

Biochemical and Biophysical Research Communications, Год журнала: 2025, Номер unknown, С. 151890 - 151890

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Thiol-chromene click reaction-triggered mitochondria-targeted ratiometric fluorescent probe for intracellular biothiol imaging DOI
Aishan Ren,

Lige Qiao,

Kechun Li

и другие.

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Авг. 30, 2024

Язык: Английский

Процитировано

2
TDDFT elucidating the PET-Inhibition Mediated fluorescence enhancement in a Cysteine probe DOI

You-Qian Ma,

Hang Zhang, Yan Zhang

и другие.

Chemical Physics Letters, Год журнала: 2024, Номер 856, С. 141683 - 141683

Опубликована: Окт. 11, 2024

Язык: Английский

Процитировано

1
Simple, Rapid and Enzyme-Free Assay for Potentiometric Determination of L-Cysteine Based on Meso-2,3-Dimercaptosuccinic Acid Self-Assembled Gold Electrode DOI

Hong-Shu Xiao,

Bowen Wu, Bin Li

и другие.

Опубликована: Янв. 1, 2024

As a sulfur containing α-amino acid in nature, L-cysteine (L-Cys) plays crucial role variety of metabolic pathways with many important physiological functions. However, it is still great challenge to construct simple and rapid approach for L-Cys up now. In this paper, simple, enzyme-free method was developed potentiometric determination by using meso-2, 3-dimercaptosuccinic (DMSA) self-assembled monolayer on gold electrode (Au/DMSA). The surface morphology property membrane without combined were characterized means scanning electron microscopy, X-ray photoelectron spectroscopy, electrochemical impedance cyclic voltammetry, respectively. recognition mechanism may be attributed formation hydrogen bonds between DMSA the target molecule L-Cys, producing double electric layer at sensing interface, that potential decreases increasing concentration L-Cys. assay results showed good linear response Tris-HCl buffer solution (pH=7.0) obtained range 1.0×10-8 1.0×10-3 mol/L, slope -57.00±0.63 mV/-pc (25ºC) detection limit 7.9×10-9 mol/L. possessed fast time (45s), reproducibility, strong anti-interference performance. Moreover, can well applied pig serums recovery rate 94.3~106.3%, indicating promising application prospect.

Язык: Английский

Процитировано

0