Single‐Cell Liquid‐Core Microcapsules for Biomedical Applications
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 24, 2025
Abstract
More
recently,
single‐cell
encapsulation
emerged
as
a
promising
field
in
biomedicine
due
to
its
potential
applications,
cell
analysis
and
therapy.
Traditional
techniques
involve
embedding
cells
crosslinked
polymers
create
continuous
microgels,
suitable
mainly
for
adherent
cells,
or
encapsulating
them
droplets
only
short‐term
analysis,
their
instability.
In
this
study,
we
developed
method
single
liquid‐core
microcapsules
address
these
limitations.
The
liquid
system
is
generated
an
all
aqueous
environment
through
polymeric
electrostatic
interactions.
Additionally,
design
innovative
low
cost
sorting
utilizing
magnetic
nanoparticles
(MNPs)
efficiently
select
encapsulated
units
further
applications.
This
tested
with
both
suspension
types,
demonstrating
cytocompatibility
no
abnormal
effects
on
behavior.
MNP‐based
achieved
nearly
80%
purity
of
the
population.
Overall,
technology
provides
highly
efficient
such
screening,
by
enabling
precise
short
medium‐term
real
time
monitoring,
high
resolution
imaging
cellular
Furthermore,
semipermeable
membrane
unlocks
new
advancing
therapy
offering
protection
while
ensuring
diffusion
therapeutic
factors,
paving
way
strategies.
Язык: Английский
Liquid Biopsy Instrument for Ultra-Fast and Label-Free Detection of Circulating Tumor Cells
Zhu Shu,
Zhixian Zhu,
Chen Ni
и другие.
Research,
Год журнала:
2024,
Номер
7
Опубликована: Янв. 1, 2024
Rapid
diagnosis
and
real-time
monitoring
are
of
great
important
in
the
fight
against
cancer.
However,
most
available
diagnostic
technologies
time-consuming
labor-intensive
commonly
invasive.
Here,
we
describe
CytoExam,
an
automatic
liquid
biopsy
instrument
designed
based
on
inertial
microfluidics
impedance
cytometry,
which
uses
a
deep
learning
algorithm
for
analysis
circulating
tumor
cells
(CTCs).
In
silico
vitro
experiments
demonstrated
that
CytoExam
could
achieve
label-free
detection
CTCs
peripheral
blood
cancer
patients
within
15
min.
The
clinical
applicability
was
also
verified
using
samples
from
10
healthy
donors
>50
with
breast,
colorectal,
or
lung
Significant
differences
number
collected
predicted
were
observed
between
2
groups,
variations
dielectric
properties
being
observed.
ultra-fast
minimally
invasive
features
may
pave
way
new
paths
scientific
research.
Язык: Английский
Dynamically Tunable Optofluidic Multifocal Microlens Arrays by 3D Printing
ACS Sensors,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 4, 2025
Microlens
arrays
(MLAs)
are
key
components
in
3D
integrated
imaging
optical
systems,
particularly
the
multifocal
MLAs,
which
provide
a
new
strategy
to
break
through
depth-of-field
limitations
for
imaging.
However,
focal
lengths
of
most
existing
MLAs
that
produced
by
solid
materials
fixed,
making
it
difficult
meet
dynamic
requirements
with
large
depth
field.
In
this
article,
we
innovatively
propose
dynamically
tunable
using
fluid
as
lens
material,
is
into
three-dimensional
optofluidic
chip
fabricated
two-photon
printing
technology.
The
realized
filling
microcavity
array
flow
streams
gradient
refractive
index
(RI)
distribution,
formed
convection
and
diffusion
between
miscible
liquids
different
RIs.
By
changing
rates,
RI
distribution
can
be
readily
regulated;
thus,
characteristics
tuned.
modulation
mechanism
revealed
combining
theoretical
analysis,
numerical
simulations,
experimental
observations.
Thanks
excellent
regulatability
fluids
optofluidics,
present
MLA
offers
wide
adjustment
range
length,
aperture,
spot
intensity.
Especially,
possesses
ability
rapidly
switch
planes
(flat,
concave,
multiple-curved
planes).
Furthermore,
applications
demonstrated
fluorescent
microparticles
fluorescence-stained
cells
samples,
exhibit
enhanced
magnification
improved
clarity.
This
adaptability
supports
sample
observation,
highlighting
great
potential
requiring
Язык: Английский
The use of droplet-based microfluidic technologies for accelerated selection of Yarrowia lipolytica and Phaffia rhodozyma yeast mutants
Biology Methods and Protocols,
Год журнала:
2024,
Номер
9(1)
Опубликована: Янв. 1, 2024
Abstract
Microorganisms
are
widely
used
for
the
industrial
production
of
various
valuable
products,
such
as
pharmaceuticals,
food
and
beverages,
biofuels,
enzymes,
amino
acids,
vaccines,
etc.
Research
is
constantly
carried
out
to
improve
their
properties,
mainly
increase
productivity
efficiency
reduce
cost
processes.
The
selection
microorganisms
with
improved
qualities
takes
a
lot
time
resources
(both
human
material);
therefore,
this
process
itself
needs
optimization.
In
last
two
decades,
microfluidics
technology
appeared
in
bioengineering,
which
allows
manipulating
small
particles
(from
tens
microns
nanometre
scale)
flow
liquid
microchannels.
based
on
small-volume
objects
(microdroplets
from
nano
femtolitres),
manipulated
using
microchip.
chip
made
an
optically
transparent
inert
medium
material
contains
series
channels
size
(<1
mm)
certain
geometry.
Based
physical
chemical
properties
microparticles
(like
size,
weight,
optical
density,
dielectric
constant,
etc.),
they
separated
microsensors.
idea
accelerated
application
microfluidic
technologies
separate
mutants
after
mutagenesis.
This
article
discusses
possible
practical
implementation
separation
mutants,
including
yeasts
like
Yarrowia
lipolytica
Phaffia
rhodozyma
mutagenesis
will
be
discussed.
Язык: Английский
Lab-In-Fiber Optofluidic Device for Droplet Digital Polymerase Chain Reaction (DdPCR) with Real-Time Monitoring
ACS Sensors,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 25, 2024
Droplet
microfluidic
systems
have
emerged
as
indispensable
and
advanced
tools
in
contemporary
biological
science.
A
prominent
example
is
the
droplet
digital
polymerase
chain
reaction
(ddPCR),
which
plays
a
pivotal
role
next-generation
sequencing
detection
of
rare
nucleic
acids
or
mutations.
However,
existing
optical
configurations
are
bulky,
intricate,
costly,
require
meticulous
alignment
to
optimize
fluorescence
sensing.
Herein,
we
propose
lab-in-fiber
optofluidic
system
(LiFO),
provides
stable
compact
footprint,
self-alignment,
enhanced
coupling
for
high-accuracy
ddPCR.
Moreover,
LiFO
could
expand
its
capabilities
multiangle-scattering
light
collection
collect
focused
forward-scattering
(fFSL)
enable
real-time
counting
size
monitoring.
To
accomplish
these
attributes,
incorporates
fibers,
along
with
fabricated
PDMS
grooves,
self-aligned
setup
implement
simultaneous
scattering
detection.
Furthermore,
harnesses
concept
flowing
droplets
functioning
microlenses,
allows
us
translate
fFSL
signals
into
information.
We
demonstrated
effectiveness
ddPCR
applications,
illustrating
capacity
enhance
accuracy
precision
DNA
quantification.
Notably,
exhibits
improved
linearity
measurement
serial
dilutions,
reflected
by
an
increase
Язык: Английский