Application of CRISPR-Cas System in HPV Detection Using Biosensor Devices and POC Technologies

BME Frontiers, Год журнала: 2025, Номер 6

Опубликована: Янв. 1, 2025

Human papillomavirus (HPV) is the most common virus for genital tract infections. Cervical cancer ranks as fourth prevalent globally, with over 99% of cases in women attributed to HPV infection. This infection continues pose an ongoing threat public health. Therefore, development rapid, high-throughput, and sensitive detection platforms important, especially regions limited access advanced medical resources. CRISPR-based biosensors, a promising new method nucleic acid detection, are now rapidly widely used basic applied research have received much attention recent years diagnosis treatment. In this review, we discuss mechanisms functions CRISPR-Cas system, focusing on its applications diagnostics. The review covers CRISPR technologies such CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, along amplification methods, signal output systems, point-of-care testing (POCT) strategies. comprehensive overview highlights versatility potential detection. We also numerous biosensors developed since introduction detect HPV. Finally, some challenges faced by system.

Язык: Английский

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Double-emulsion droplet digital CRISPR/Cas12a for amplification-free, absolute quantification of nucleic acids at attomole levels

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162098 - 162098

Опубликована: Апрель 1, 2025

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PAM triggered visual “green-yellow-orange” ratiometric fluorescence probes in CRISPR/Cas12a: Universal detection of exosome and APE1

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 114010 - 114010

Опубликована: Май 1, 2025

Язык: Английский

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Screening and selection of cellulase-secreting yeast single cells using integrated double emulsion droplet and flow cytometry techniques

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 416, С. 136038 - 136038

Опубликована: Май 29, 2024

The capability of yeast cell factories to secrete industrially important enzymes into the extracellular environment can significantly reduce overall production costs. This impacts a wide range sectors, including pharmaceuticals, food and feed, textiles, detergents, materials, energy. Although recent advances in molecular biology laboratory automation have expanded our construct enormous strain variant libraries, it calls for technologies effectively screen thousands strains with phenotypes that are spatially removed from original cell. In this work, we demonstrate application integrated double emulsion (DE) droplets fluorescence-activated sorting (FACS) technology screening selection single cells based on enzyme activity. Single Saccharomyces cerevisiae secreting cellulase enzyme, β-glucosidase, compartmentalised within highly monodisperse DE fluorogenic substrate. After single-cell encapsulation, cultivated allow production. cell-laden sorted by FACS identity high β-glucosidase secretion. Moreover, encapsulated S. successfully recovered DEs after sorting. We expect user-friendly method will be combined synthetic techniques optimise value-added product yield stability.

Язык: Английский

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Circular DNA enhanced amplification-free CRISPR/Cas12a assays for end-user friendly ultra-sensitive Porphyromonas gingivalis diagnosis

Microchemical Journal, Год журнала: 2024, Номер unknown, С. 111983 - 111983

Опубликована: Окт. 1, 2024

Язык: Английский

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