Label-Free Liquid Crystal Biosensor Based on Split Aptamers for Detecting Serum Myoglobin

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

Myoglobin (Mb) is a critical cytoplasmic heme protein and one of the earliest detectable biomarkers following acute myocardial infarction (MI). The rapid precise detection serum Mb holds significant clinical value. This study capitalizes on advantages split aptamer (Apt) sequence, which short effectively minimizes false-positive signals caused by secondary structure probe. It constructs "sandwich"-structured liquid crystal biosensor with an "Apt-target molecule-Apt" configuration for label-free Mb. Apt fragments were immobilized surface glass substrate as capture probes (CP) using triethoxybutanal silane (TEA) linker molecules. When present, other fragment, signal probe (SP), specifically binds reassociates CP, restoring original stem-loop Apt, thereby inducing change in alignment 5CB leads to variations brightness color optical polarizing microscope image, facilitating quantitative method capable detecting concentrations ranging from 0.05 130 ng/mL, minimum limit 0.045 ng/mL. sandwich biosensing offers several advantages, including detection, operational simplicity, high specificity. demonstrates potential biomarkers.

Язык: Английский

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Smart Textile Electrochemical Capacitive Biosensor for Real-Time Monkeypox Virus Detection

ACS Applied Electronic Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 21, 2025

Язык: Английский

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Research Progress of CRISPR-Cas System in Medical Diagnosis

Journal of Clinical Personalized Medicine, Год журнала: 2025, Номер 04(02), С. 231 - 239

Опубликована: Янв. 1, 2025

Язык: Английский

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