A Novel Method for Detecting Genetic Biomarkers in Blood-based Liquid Biopsies Using Surface Plasmon Resonance Imaging and Magnetic Beads Shows Promise in Cancer Diagnosis and Monitoring

Talanta, Год журнала: 2025, Номер unknown, С. 127543 - 127543

Опубликована: Янв. 1, 2025

Язык: Английский

---

Exploring the potential of ionic liquid-based electrochemical biosensors for real-time biomolecule monitoring in pharmaceutical applications: From lab to life

Results in Engineering, Год журнала: 2023, Номер 20, С. 101533 - 101533

Опубликована: Окт. 21, 2023

Ionic liquid-based electrochemical biosensors have recently surged to prominence as an intriguing technology with transformative potential for real-time biomolecule monitoring, notably within the dynamic pharmaceutical landscape. By demonstrating their adeptness in detecting extensive array of biomolecules, encompassing glucose, hormones, nucleic acids, and pivotal biomarkers, these substantiated efficacy monitoring biomolecules sphere revealing excellent thermal stability, minimal volatility, expansive working range. For instance, biosensor comprising a conducting polymer, graphene, gold nanoparticles, ionic liquids exhibited exceptional sensitivity, limit detection low 1 fM (at S/N = 3), range 3.2 0.32 pM, remarkable long-term durability aflatoxin B1 detection. In light compelling developments, first time, this review offers comprehensive exploration recent advancements emergent trajectories (spanning majorly from 2019 2023) utilization biosensors, particularly context aforementioned diverse pertinent industry. Furthermore, it discusses challenges opportunities that lie ahead production shedding on reshape future applications.

Язык: Английский

---

High detectable and low sample volume portable cholesterol biosensor based on PAMAM dendrimers grafted to bioinspired polynorepinephrine nanomaterials

Journal of Applied Electrochemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Язык: Английский

---

Designing a Simple Electrochemical Genosensor for the Detection of Urinary PCA3, a Prostate Cancer Biomarker

Micromachines, Год журнала: 2024, Номер 15(5), С. 602 - 602

Опубликована: Апрель 29, 2024

This study investigates the feasibility of a simple electrochemical detection Prostate Cancer Antigen 3 (PCA3) fragments extracted from patients’ urine, using thiolated single-strand DNA probe immobilized on gold surface without redox probe. To enhance PCA3 recognition process, we conducted comparative analysis hybridization location two probes: Probe 1 targets first 40 bases, while 2 fragment bases 47 to 86. Hybridization with followed, square wave voltammetry. The limit designed genosenors were order (2.2 ng/mL), and (1.6 ng/mL) for Probes 2, respectively, subsequent sensitivities (0.09 ± 0.01) µA−1 · µg−1 mL (0.10 mL. Specificity tests then sensor functionalized as it presents better analytical performances. results indicate that can clearly discriminate complementary target non-complementary one. A further modeling calibration curves Power Law/Hill model indicates dissociation constant increases by one magnitude, confirming ability perfectly ones.

Язык: Английский

---

Colorimetric detection of single nucleotide polymorphisms mediated by unmodified gold nanoparticles and peptide nucleic acids

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113773 - 113773

Опубликована: Апрель 1, 2025

Язык: Английский

---

First electrochemical bioplatform to assist in personalized 5-fluorouracil chemotherapy

Sensors and Actuators B Chemical, Год журнала: 2023, Номер 401, С. 135017 - 135017

Опубликована: Ноя. 22, 2023

Язык: Английский

---

High-sensitivity label-free electrochemical genosensors for carbon nanotube plasmon-assisted detection of somatic mutations in nucleic acids from formalin-fixed paraffin-embedded tissues

Microchemical Journal, Год журнала: 2024, Номер unknown, С. 112234 - 112234

Опубликована: Ноя. 1, 2024

Язык: Английский

---

A novel magnetic ligand-based assay for the electrochemical determination of BRD4

Talanta, Год журнала: 2024, Номер 279, С. 126577 - 126577

Опубликована: Июль 19, 2024

The first magnetic ligand-based electrochemical assay aimed at the determination of BRD4 was developed and validated. is an epigenetic regulator great interest in oncology relation to its overexpression observed pathogenesis several cancer diseases. also represents a major target for development innovative treatments protein inhibition or degradation. Despite relevance both diagnostics therapeutic purposes, current methodologies are limited commercial ELISA kits. We present novel BRD4. based on use small synthetic fragment natural ligand as receptor, thus exploiting intrinsic biological protein-protein recognition mechanism. In addition, features beads immobilization platforms peroxidase-conjugated monoclonal anti-BRD4 antibody generation signal. shows outstanding performance terms rapidity, with results achievable less than 20 min, no matrix effect when applied human plasma cell lysate samples, excellent specificity. proposed method exhibits limit detection 2.66 nM response range tunable function amount immobilized receptor. successfully accurate untreated lysates, proven by reference method. good bioassay showed potential application this strategy convenient point-of-care testing.

Язык: Английский

---

Tuning assembled DNA nanoarchitecture into disassembled state to enhance accurate and ultrasensitive detection of KRAS G12D mutation

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 420, С. 136513 - 136513

Опубликована: Авг. 23, 2024

Язык: Английский

---