Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation DOI Open Access

Karlo Grgurević,

Dora Bramberger,

Martina Miloloža

и другие.

Polymers, Год журнала: 2024, Номер 16(23), С. 3407 - 3407

Опубликована: Дек. 3, 2024

The environmental impact of plastic waste is a growing global challenge, primarily due to non-biodegradable plastics from fossil resources that accumulate in ecosystems. Biodegradable polymers like polyhydroxyalkanoates (PHAs) offer sustainable alternative. PHAs are microbial biopolymers produced by microorganisms using renewable substrates, including agro-industrial byproducts, making them eco-friendly and cost-effective. This study focused on the isolation characterization PHA-producing waste, chickpeas, chickpeas with bean residues, starch. Screening via Sudan Black staining identified PHA-accumulating strains such as

Язык: Английский

Biological delignification and anaerobic fermentation of wheat straw: A promising approach for sustainable utilization of crop straw bioresources DOI Creative Commons
Mudasir Nazar, Jipeng Tian, Xin Wang

и другие.

Industrial Crops and Products, Год журнала: 2025, Номер 227, С. 120839 - 120839

Опубликована: Март 15, 2025

Язык: Английский

Процитировано

1

Investigating chemical pre-treatment methods: Valorization of wheat straw to enhance polyhydroxyalkanoate (PHA) production with novel isolate Bacillus paranthracis RSKS-3 DOI Creative Commons
Rohan Samir Kumar Sachan, Inderpal Devgon, Vikas Sharma

и другие.

Heliyon, Год журнала: 2024, Номер 10(11), С. e31572 - e31572

Опубликована: Май 22, 2024

Wheat is a crucial food crop worldwide, generating straw upon post-harvest. The often burned to enhance soil fertility, leading massive air pollution. In this study, wheat was investigated for the production of Polyhydroxyalkanoate (PHA) using novel isolate Bacillus paranthracis RSKS-3. pulverized and valorized with different acids (2% 4% H2SO4, acetic acid, hydrochloric acid) alkalis NaOH, calcium carbonate, potassium hydroxide). validation carbohydrates done Molisch test by analyzing purple-ring DNS which concluded H2SO4 as an effective treatment maximal sugar yield 5.04 mg mL-1 at P<0.05. bioconversion efficiency extract PHA resulted in 0.87 g L-1 RSKS-3, later characterized Ultraviolet (UV)-spectroscopy FT-IR assessment. findings research offer potential strategy mitigate airborne pollutants that result from smouldering straw, thereby contributing significant improvements sustainable development.

Язык: Английский

Процитировано

4

Screening and characterization of PHA producing bacteria from sewage water identifying Bacillus paranthracis RSKS-3 for bioplastic production DOI Creative Commons
Rohan Samir Kumar Sachan, Abhinav Kumar, Arun Karnwal

и другие.

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 14, 2025

Abstract Polyhydroxyalkanoate (PHA) as bioplastic is considered a replacement for conventional plastic due to its more beneficial properties. The ability of PHA biodegrade in shorter period major advantage. Different sewage water samples were collected from the Budha Nala near Maheru regions Punjab. PHA-producing bacteria isolated using minimal salt media supplemented with Nile blue. Further screening was carried out Sudan Black B stain and red stain. positive isolates characterized gram reaction, motility, biochemical tests. individual later screened maximum accumulation glucose. extracted FTIR, XRD, SEM, UV spectroscopy, NMR, TGA. Twenty-six different on Upon stain, nineteen showed black granules orange fluorescence bodies under 100X magnification that confirmed polyhydroxyalkanoates. tests partially belonging Bacillus genus. All produced granular form, however, isolate P-3 production 0.068 g/L. FTIR XRD chemical crystallinity studies spectroscopy by analyzing absorption spectra at 235 nm standard crotonic acid sulfuric conversion acid. P-3, paranthracis RSKS-3 first reported bacterium produce necessary optimize efficiency yield.

Язык: Английский

Процитировано

0

Rice Straw Hydrolysate Valorization for Efficient Polyhydroxyalkanoate Production in a Two-Stage Process DOI Creative Commons

Fanirintsoa Herivonona Andrianantenaina,

Raj Morya,

Hwan-Hong Joo

и другие.

Environmental Technology & Innovation, Год журнала: 2025, Номер unknown, С. 104217 - 104217

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Waste to wealth: Polyhydroxyalkanoates (PHA) production from food waste for a sustainable packaging paradigm DOI Creative Commons
Heri Septya Kusuma,

Atna Sabita,

Najla Anira Putri

и другие.

Food Chemistry Molecular Sciences, Год журнала: 2024, Номер 9, С. 100225 - 100225

Опубликована: Окт. 10, 2024

The growing demand for sustainable food packaging and the increasing concerns regarding environmental pollution have driven interest in biodegradable materials. This paper presents an in-depth review of production Polyhydroxyalkanoates (PHA), a polymer, from waste. PHA-based bioplastics, particularly when derived low-cost carbon sources such as volatile fatty acids (VFAs) waste oils, offer promising solution reducing plastic enhancing sustainability. Through optimization microbial fermentation processes, PHA can achieve significant efficiency improvements, with yields reaching up to 87 % content under ideal conditions. highlights technical advancements using packaging, emphasizing its biodegradability, biocompatibility, potential serve alternative petroleum-based plastics. However, challenges high costs, mechanical limitations, need scalability remain barriers industrial adoption. future hinges on overcoming these through further research innovation techniques, material properties, cost reduction strategies, along necessary legislative support promote widespread use.

Язык: Английский

Процитировано

3

Cascading Recovery of Added-Value Cocoa Bean Shell Fractions Through Autohydrolysis Treatments DOI Creative Commons
Marta Sánchez, Pedro Ferreira-Santos, Joana S. Gomes-Dias

и другие.

Food and Bioprocess Technology, Год журнала: 2024, Номер unknown

Опубликована: Июль 16, 2024

Abstract In this work, an autohydrolysis treatment was applied to cocoa bean shells (CBS) obtain different potentially added-value fractions rich in phenolic compounds with antioxidant potential and oligosaccharides prebiotic properties. The final residue enzymatically treated deliver sugars that can undergo fermentation-based biotransformation. This hydrothermal pretreatment assessed for maximum temperatures ( T max ) between 120 200 °C severities S 0 1.1 3.4. highest oligosaccharide concentration (5.5 g/L) achieved at of increase during the process allowed recovery interesting bioactive compounds, achieving a TPC activity 2.8 g/L 17178.5 µmol Fe 2+ /L, respectively, when reached °C. However, temperature, significant amount degradation products such as organic acids HMF already formed, compromise temperature 160 chosen further tests. It possible glucose yield 71% pretreated solids were hydrolysed. Hence, use autohydrolysis, avoiding toxic chemicals, has proved be sustainable alternative CBS composition employed multiple sectors.

Язык: Английский

Процитировано

2

Moderately halophilic bacterium Halomonas alkalicola strain Ext as a platform for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production with fruit peels residues as sole carbon source DOI Creative Commons
Martin N. Muigano,

Justus Onguso,

Sylvester Anami

и другие.

The Microbe, Год журнала: 2024, Номер 4, С. 100153 - 100153

Опубликована: Авг. 29, 2024

Polyhydroxyalkanoates (PHAs) are synthesized by a variety of microorganisms as intracellular storage granules under imbalanced nutrition and excess carbon. Carbon sources key contributors to the high cost PHA production, hence need for exploration cheap sustainable raw materials bacteria fermentation. In present study, Halomonas alkalicola strain Ext isolated from hypersaline lake in Kenya was assessed its ability utilize fruit peels hydrolysates (FPH) sole carbon production. Sugars were extracted dried banana, mango, orange, pineapple fruits through mechanical pretreatment dilute acid hydrolysis. Fruit pretreated with 3 % H2SO4 at 121℃ utilized shake flask fermentation produce PHAs Ext. At optimal C:N ratios between 20:1 30:1, bacterium could up 0.45±0.03, 0.394±0.12, 0.39±0.05, 0.28±0.0 g/L orange peels, mango banana respectively. A maximum content 16.92 achieved on 4 substrate loading. Monomer analysis gas chromatography-mass spectrometry (GC-MS) revealed that produced copolymer Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) 3-hydroxyvalerate (3HV) contents 5.77 %, 6.08 6.79 6.845 substrates, The findings this study suggest waste is potential feedstock production alkalicola.

Язык: Английский

Процитировано

1

Unlocking sustainable solutions: wood waste as a novel substrate for polyhydroxybutyrate (PHB) production to combat plastic pollution DOI
Mukesh Kumar, Rohan Samir Kumar Sachan,

Simran Kauts

и другие.

Environmental Technology, Год журнала: 2024, Номер unknown, С. 1 - 13

Опубликована: Окт. 10, 2024

Polyhydroxybutyrate (PHB) is considered as a hope for bioplastic production, which can serve sustainable alternative. Utilizing feedstock substrate widely explored the production but wood waste, abundant in cellulose, hemicellulose and lignocellulose, has limited studies PHB production. Herein, waste used biobased Hydrolyses of was done using sulphuric acid (H

Язык: Английский

Процитировано

1

Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation DOI Open Access

Karlo Grgurević,

Dora Bramberger,

Martina Miloloža

и другие.

Polymers, Год журнала: 2024, Номер 16(23), С. 3407 - 3407

Опубликована: Дек. 3, 2024

The environmental impact of plastic waste is a growing global challenge, primarily due to non-biodegradable plastics from fossil resources that accumulate in ecosystems. Biodegradable polymers like polyhydroxyalkanoates (PHAs) offer sustainable alternative. PHAs are microbial biopolymers produced by microorganisms using renewable substrates, including agro-industrial byproducts, making them eco-friendly and cost-effective. This study focused on the isolation characterization PHA-producing waste, chickpeas, chickpeas with bean residues, starch. Screening via Sudan Black staining identified PHA-accumulating strains such as

Язык: Английский

Процитировано

1