Aptamer based immunotherapy: a potential solid tumor therapeutic

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 17, 2025

Aptamer-based immunotherapy can be a new hope for treating solid tumors with personalized and specific approaches toward cancer therapies. Aptamers are small synthetic single-stranded nucleic acids that may bring in paradigm shift tumors. These highly selective drugs applied cellular immunotherapy, cytokine modulation, immune checkpoint suppression. This review provides an overview of the recent advances aptamer-based technologies key clinical trials involving AON-D21 AM003. potently active regulation tumor targeting. However, aptamer stability bioavailability seriously compromised by issues relating to renal clearance rapid degradation through nucleases. The latter reviewed here along novel improvements, some which involve chemical modifications greatly enhance prolong circulation time; exemplary such PEGylation, cholesterol conjugation, synthesis circular acids. regulatory aspect is also crucial. For example, addition strategies prevent drug-drug interactions (DDIs) remediation medications, this paper underscores need risk assessment, particularly because immunogenicity organ failure. use aptamers expanded development SOMAmers, X-aptamers, bioinformatics. To make major part treatment, future research should concentrate more on resolving existing expanding their beneficial uses.

Язык: Английский

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Advances with radiopharmaceutical therapy in cancer

Elsevier eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Migrasomes: key players in immune regulation and promising medical applications

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Май 15, 2025

Migrasomes are newly discovered extracellular organelles released by migrating cells, such as immune tumor and other special functional cells like podocytes embryonic cells. They contain a diverse array of constituents, including proteins, lipids, RNA which can be to the designated location activate surrounding thereby facilitating intercellular communication signal transduction. Since then, our understanding mechanism function migrasomes has expanded exponentially, with recent evidence indicating they involved in various physiological pathological processes, particularly regulation. Furthermore, methods techniques for extracting, detecting, characterizing constantly advancing. Herein, we summarize current their key roles modulating responses, well prospective challenges clinical application, aiming provide novel insights into emerging organelles.

Язык: Английский

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Site-specific aptamer immobilization via amine-to-thiol cross-linking on field-effect transistor biosensor for cortisol detection

Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер unknown, С. 114842 - 114842

Опубликована: Июнь 1, 2025

Язык: Английский

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APTAMER TABANLI TANIMLAMA YÖNTEMİ VE GIDA GÜVENLİĞİNDEKİ UYGULAMALARI

GIDA / THE JOURNAL OF FOOD, Год журнала: 2024, Номер 49(3), С. 536 - 553

Опубликована: Май 22, 2024

Aptamerler, tek sarmallı, kısa, sentetik nükleik asit dizileridir. Çeşitli hedef moleküllere karşı yüksek afinite gösteren ve spesifik olarak bağlanan aptamerler, gıdalarda bulunabilecek patojen mikroorganizmalar, biyotoksinler, alerjenler, pestisitler ağır metaller gibi çeşitli risk faktörlerinin hassas bir şekilde tespiti için kullanılmaktadır. Gıda güvenliği açısından oluşturan aptamerlerin seçilmesi SELEX adı verilen süreçle gerçekleştirilir. Seçilen aptamer, molekül ile özgün etkileşime girer bu etkileşim elektrokimyasal, optik veya diğer biyosensör teknikler kullanılarak ölçülür. analizlerinde kullanılan geleneksel yöntemlere kıyasla daha hızlı sonuçlar veren, özgüllüğe duyarlılığa sahip aptamer tabanlı duyulan ilgi giderek artmaktadır. Bu derlemede genel özellikleri prensibiyle üretimleri özetlenmiş gıda alanındaki uygulamalarına örnekler verilmiştir.

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Electrochemiluminescence-Based Lateral Flow Assay for Detection of Cardiac Troponin T Using Aptamers Developed through a Modified SELEX Technique

ACS Applied Optical Materials, Год журнала: 2024, Номер 2(8), С. 1688 - 1698

Опубликована: Авг. 13, 2024

Rapid and sensitive detection of cardiac troponin T (cTnT) through a portable device holds immense significance for the early diagnosis acute myocardial infarction in point-of-care settings. Herein, an electrochemiluminescence-based lateral flow assay (LFA) on microfluidic hybrid platform cTnT was developed using aptamer sandwich assay. Aptamers specific to were enriched from library new centrifugal-systematic evolution ligands by exponential enrichment (c-SELEX) technique. Two high-affinity aptamers, cT21 (Kd = 10.7 μM) cT33 13.1 μM), with Kd values determined isothermal titration calorimetry, used respectively as capture probes In assay, labeled Lumidot, while immobilized LFA strip cTnT–cT33–Lumidot complex. The reaction solution containing luminol cT33–Lumidot conjugate generated ECL signals under applied potential 1.4 V electrode pair screen printed nitrocellulose membrane device. intensity correlated linearly concentration range 100 pM 10 nM offered limit 142.71 pM. This sensor exhibits high prospects practical applications diagnosing

Язык: Английский

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Development of a Molecular Beacon-Based Genosensor for Detection of Human Rotavirus A

Molecular Biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 31, 2024

Язык: Английский

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