Aptamer based immunotherapy: a potential solid tumor therapeutic
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 17, 2025
Aptamer-based
immunotherapy
can
be
a
new
hope
for
treating
solid
tumors
with
personalized
and
specific
approaches
toward
cancer
therapies.
Aptamers
are
small
synthetic
single-stranded
nucleic
acids
that
may
bring
in
paradigm
shift
tumors.
These
highly
selective
drugs
applied
cellular
immunotherapy,
cytokine
modulation,
immune
checkpoint
suppression.
This
review
provides
an
overview
of
the
recent
advances
aptamer-based
technologies
key
clinical
trials
involving
AON-D21
AM003.
potently
active
regulation
tumor
targeting.
However,
aptamer
stability
bioavailability
seriously
compromised
by
issues
relating
to
renal
clearance
rapid
degradation
through
nucleases.
The
latter
reviewed
here
along
novel
improvements,
some
which
involve
chemical
modifications
greatly
enhance
prolong
circulation
time;
exemplary
such
PEGylation,
cholesterol
conjugation,
synthesis
circular
acids.
regulatory
aspect
is
also
crucial.
For
example,
addition
strategies
prevent
drug-drug
interactions
(DDIs)
remediation
medications,
this
paper
underscores
need
risk
assessment,
particularly
because
immunogenicity
organ
failure.
use
aptamers
expanded
development
SOMAmers,
X-aptamers,
bioinformatics.
To
make
major
part
treatment,
future
research
should
concentrate
more
on
resolving
existing
expanding
their
beneficial
uses.
Язык: Английский
Advances with radiopharmaceutical therapy in cancer
Elsevier eBooks,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Migrasomes: key players in immune regulation and promising medical applications
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Май 15, 2025
Migrasomes
are
newly
discovered
extracellular
organelles
released
by
migrating
cells,
such
as
immune
tumor
and
other
special
functional
cells
like
podocytes
embryonic
cells.
They
contain
a
diverse
array
of
constituents,
including
proteins,
lipids,
RNA
which
can
be
to
the
designated
location
activate
surrounding
thereby
facilitating
intercellular
communication
signal
transduction.
Since
then,
our
understanding
mechanism
function
migrasomes
has
expanded
exponentially,
with
recent
evidence
indicating
they
involved
in
various
physiological
pathological
processes,
particularly
regulation.
Furthermore,
methods
techniques
for
extracting,
detecting,
characterizing
constantly
advancing.
Herein,
we
summarize
current
their
key
roles
modulating
responses,
well
prospective
challenges
clinical
application,
aiming
provide
novel
insights
into
emerging
organelles.
Язык: Английский
Development of a Molecular Beacon-Based Genosensor for Detection of Human Rotavirus A
Molecular Biotechnology,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 31, 2024
Язык: Английский
APTAMER TABANLI TANIMLAMA YÖNTEMİ VE GIDA GÜVENLİĞİNDEKİ UYGULAMALARI
GIDA / THE JOURNAL OF FOOD,
Год журнала:
2024,
Номер
49(3), С. 536 - 553
Опубликована: Май 22, 2024
Aptamerler,
tek
sarmallı,
kısa,
sentetik
nükleik
asit
dizileridir.
Çeşitli
hedef
moleküllere
karşı
yüksek
afinite
gösteren
ve
spesifik
olarak
bağlanan
aptamerler,
gıdalarda
bulunabilecek
patojen
mikroorganizmalar,
biyotoksinler,
alerjenler,
pestisitler
ağır
metaller
gibi
çeşitli
risk
faktörlerinin
hassas
bir
şekilde
tespiti
için
kullanılmaktadır.
Gıda
güvenliği
açısından
oluşturan
aptamerlerin
seçilmesi
SELEX
adı
verilen
süreçle
gerçekleştirilir.
Seçilen
aptamer,
molekül
ile
özgün
etkileşime
girer
bu
etkileşim
elektrokimyasal,
optik
veya
diğer
biyosensör
teknikler
kullanılarak
ölçülür.
analizlerinde
kullanılan
geleneksel
yöntemlere
kıyasla
daha
hızlı
sonuçlar
veren,
özgüllüğe
duyarlılığa
sahip
aptamer
tabanlı
duyulan
ilgi
giderek
artmaktadır.
Bu
derlemede
genel
özellikleri
prensibiyle
üretimleri
özetlenmiş
gıda
alanındaki
uygulamalarına
örnekler
verilmiştir.
Electrochemiluminescence-Based Lateral Flow Assay for Detection of Cardiac Troponin T Using Aptamers Developed through a Modified SELEX Technique
ACS Applied Optical Materials,
Год журнала:
2024,
Номер
2(8), С. 1688 - 1698
Опубликована: Авг. 13, 2024
Rapid
and
sensitive
detection
of
cardiac
troponin
T
(cTnT)
through
a
portable
device
holds
immense
significance
for
the
early
diagnosis
acute
myocardial
infarction
in
point-of-care
settings.
Herein,
an
electrochemiluminescence-based
lateral
flow
assay
(LFA)
on
microfluidic
hybrid
platform
cTnT
was
developed
using
aptamer
sandwich
assay.
Aptamers
specific
to
were
enriched
from
library
new
centrifugal-systematic
evolution
ligands
by
exponential
enrichment
(c-SELEX)
technique.
Two
high-affinity
aptamers,
cT21
(Kd
=
10.7
μM)
cT33
13.1
μM),
with
Kd
values
determined
isothermal
titration
calorimetry,
used
respectively
as
capture
probes
In
assay,
labeled
Lumidot,
while
immobilized
LFA
strip
cTnT–cT33–Lumidot
complex.
The
reaction
solution
containing
luminol
cT33–Lumidot
conjugate
generated
ECL
signals
under
applied
potential
1.4
V
electrode
pair
screen
printed
nitrocellulose
membrane
device.
intensity
correlated
linearly
concentration
range
100
pM
10
nM
offered
limit
142.71
pM.
This
sensor
exhibits
high
prospects
practical
applications
diagnosing
Язык: Английский