Experimental Study on Compatibility of Human Bronchial Epithelial Cells in Collagen–Alginate Bioink for 3D Printing
Bioengineering,
Год журнала:
2024,
Номер
11(9), С. 862 - 862
Опубликована: Авг. 23, 2024
This
paper
reports
an
experimental
study
on
the
compatibility
of
human
bronchial
epithelial
(HBE)
cells
in
a
collagen-alginate
bioink.
The
was
assessed
using
culture
well
method
with
three
bioink
compositions
prepared
from
10%
alginate
solution
and
neutralized
TeloCol-10
mg/mL
collagen
stock
solution.
Cell
viability,
quantified
by
(live
cell
count-dead
count)/live
count
within
HBE
cell-laden
hydrogel,
evaluated
live/dead
assay
Day
0
to
6.
Experimental
results
demonstrated
that
4:1
composition
exhibited
highest
viability
6
(85%),
outperforming
1:4
composition,
which
showed
75%
45%,
respectively.
Additionally,
live
for
0,
trend
persisted
through
Days
1
6,
underscoring
its
superior
performance
maintaining
promoting
proliferation.
These
findings
show
higher
compared
other
two
compositions.
Язык: Английский
The Biomedical Applications of Biomolecule Integrated Biosensors for Cell Monitoring
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(12), С. 6336 - 6336
Опубликована: Июнь 7, 2024
Cell
monitoring
is
essential
for
understanding
the
physiological
conditions
and
cell
abnormalities
induced
by
various
stimuli,
such
as
stress
factors,
microbial
invasion,
diseases.
Currently,
techniques
detecting
metabolites
originating
from
specific
cells
are
employed
to
obtain
information
on
in
terms
of
human
health.
Although
states
have
traditionally
been
accessed
using
instrument-based
analysis,
this
has
replaced
sensor
systems
equipped
with
new
materials
technologies.
Various
developed
recognizing
biological
markers
proteins
surfaces,
components
plasma
membranes,
secreted
metabolites,
DNA
sequences.
Sensor
classified
into
subclasses,
chemical
sensors
biosensors,
based
used
recognize
targets.
In
review,
we
aim
outline
fundamental
principles
cells,
encompassing
both
biosensors
sensors.
Specifically,
focus
biosensing
types
sensing
signal-transducing
elements
introduce
recent
advancements
applications
biosensors.
Finally,
address
present
challenges
biosensor
prospects
that
should
be
considered
enhance
performance.
review
covers
application
believe
it
can
provide
valuable
insights
researchers
general
readers
interested
its
further
biomedical
fields.
Язык: Английский
Effects of Coaxial Nozzle’s Inner Nozzle Diameter on Filament Strength and Gelation in Extrusion-Based 3D Printing with In Situ Ionic Crosslinking
Biomimetics,
Год журнала:
2024,
Номер
9(10), С. 589 - 589
Опубликована: Сен. 29, 2024
This
study
systematically
investigates
the
effects
of
coaxial
nozzle’s
inner
nozzle
diameter
on
strength
and
gelation
filaments
produced
via
extrusion-based
3D
printing
with
in
situ
ionic
crosslinking.
In
this
system,
bioink
(sodium
alginate
solution)
was
extruded
through
outer
nozzle,
crosslinking
solution
(calcium
chloride
nozzle.
The
fixed
at
2.16
mm,
varied
among
1.19,
0.84,
0.584
mm.
results
indicate
that,
as
decreased,
filament
became
poorer.
These
findings
highlight
importance
optimizing
for
improved
Язык: Английский
Extrusion Bioprinting of Scaffolds: An Introduction
Springer eBooks,
Год журнала:
2024,
Номер
unknown, С. 1 - 15
Опубликована: Ноя. 6, 2024
Язык: Английский
Integration of bioprinting advances and biomechanical strategies for in vitro lung modelling
Biofabrication,
Год журнала:
2024,
Номер
17(1), С. 012006 - 012006
Опубликована: Ноя. 13, 2024
Abstract
The
recent
occurrence
of
the
Covid-19
pandemic
and
frequent
wildfires
have
worsened
pulmonary
diseases
raised
urgent
need
for
investigating
host-pathogen
interactions
advancing
drug
vaccine
therapies.
Historically,
research
experimental
studies
relied
on
two-dimensional
cell
culture
dishes
and/or
animal
models,
which
suffer
from
physiological
differences
human
lung.
More
recently,
there
has
been
investigation
into
use
lung-on-a-chip
models
organoids,
while
bioprinting
technologies
also
emerged
to
fabricate
three-dimensional
constructs
or
lung
with
enhanced
relevance.
Concurrently,
achievements
made
develop
biomimetic
strategies
simulating
in
vivo
biomechanical
conditions
induced
by
breathing,
though
challenges
remain
incorporating
these
bioprinted
models.
Bioprinted
combined
advanced
would
represent
a
promising
approach
advance
disease
discovery
therapeutic
development.
As
inspired,
this
article
briefly
reviews
progress
both
vitro
strategies,
focus
native
tissue
microstructure
properties,
constructs,
mimic
environment.
This
urges
that
integration
advances
be
essential
achieve
synergistic
effects
modelling.
Key
issues
are
identified
discussed
along
recommendations
future
research.
Язык: Английский