Molecular & Cellular Oncology,
Год журнала:
2024,
Номер
11(1)
Опубликована: Ноя. 11, 2024
Cervical
cancer
(CC)
is
one
of
the
common
malignant
tumors
in
women,
and
incidence
rate
located
second
place
female
tumors.
As
a
major
RNA
N6-methyladenosine
(m6A)
methyltransferase,
methyltransferase-like
14
(METTL14)
involved
tumor
progression
by
catalyzing
methylation
modifications
mRNAs.
However,
molecular
mechanism
METTL14-mediated
m6A
modification
CC
remains
not
fully
revealed.
The
expression
METTL14
was
detected
RT-qPCR
western
blot.
Cell
function
assayed
cell
counting
kit-8
(CCK-8)
assay
flow
cytometry
analysis.
Methylated
immunoprecipitation
(MeRIP)
used
to
confirm
relationship
between
homeobox
B13
(HOXB13).
In
our
study,
we
found
that
level
elevated
tissues
cells
compared
with
their
controls.
inhibition
significantly
impaired
proliferation
epithelial-mesenchymal
transition
(EMT)
process,
while
also
induced
apoptosis
HeLa
C33A
cells.
Furthermore,
findings
indicated
(HOXB13)
target
METTL14,
which
positively
regulated
HOXB13
an
m6A-dependent
manner.
Rescue
experiments
overexpression
effectively
reversed
suppression
knockdown.
Finally,
confirmed
METTL14-modified
exerted
oncogenic
effect
through
activation
nuclear
factor
kappa
B
(NF-κB)
pathway.
conclusion,
data
demonstrated
HOXB13,
mediated
facilitated
advancement
targeting
NF-κB
pathway,
may
be
potential
for
treatment
CC.
Cell Death Discovery,
Год журнала:
2024,
Номер
10(1)
Опубликована: Авг. 26, 2024
Abstract
CAFs
(cancer-associated
fibroblasts)
are
highly
flexible
cells
of
the
cancer
microenvironment.
They
produce
extracellular
matrix
(ECM)
constituents
that
form
structure
tumor
stroma
but
also
a
source
metabolites,
growth
factors,
chemokines,
and
exosomes
impact
every
aspect
tumor,
including
its
response
to
treatment.
It
is
believed
exosomal
miRNAs
facilitate
intercellular
signaling,
which
essential
for
development
cancer.
The
role
in
microenvironment
(TME)
carcinogenesis
reviewed
this
paper.
preferred
reporting
items
systematic
reviews
meta-analyses
(PRISMA)
2020
guidelines
were
used
perform
review.
Several
databases,
Web
Science,
Medline,
Embase,
Cochrane
Library,
Scopus,
searched
using
following
keywords:
CAFs,
CAF,
cancer-associated
fibroblasts,
stromal
miRNA,
miRNAs,
exosome
similar
terms.
We
identified
studies
investigating
TME
their
carcinogenesis.
A
total
12,572
papers
identified.
After
removing
duplicates
(
n
=
3803),
8774
articles
screened
by
title
abstract.
Of
these,
421
excluded
from
further
analysis.
has
been
reported
if
not
functioning
correctly,
may
influence
secretory
phenotype
tip
contribute
increased
invasiveness,
spread,
decreased
treatment
efficacy,
poorer
prognosis.
Under
influence,
normal
fibroblasts
(NFs)
transformed
into
CAFs.
Furthermore,
they
participate
metabolic
reprogramming,
allows
fast
proliferation
cell
population,
adaptation
growing
energy
demands,
capacity
avoid
immune
system
identification.
Abstract
Background
Recent
evidence
has
demonstrated
that
abnormal
expression
and
regulation
of
circular
RNA
(circRNAs)
are
involved
in
the
occurrence
development
a
variety
tumors.
The
aim
this
study
was
to
investigate
effects
circ_PPAPDC1A
Osimertinib
resistance
NSCLC.
Methods
Human
circRNAs
microarray
analysis
conducted
identify
differentially
expressed
(DE)
Osimertinib-acquired
tissues
effect
on
cell
proliferation,
invasion,
migration,
apoptosis
assessed
both
vitro
vivo.
Dual-luciferase
reporter
assay,
RT-qPCR,
Western-blot,
rescue
assay
were
employed
confirm
interaction
between
circ_PPAPDC1A/miR-30a-3p/IGF1R
axis.
Results
results
revealed
significantly
upregulated
acquired
reduced
sensitivity
PC9
HCC827
cells
promoted
while
inhibiting
Osimertinib-resistant
PC9/OR
HCC829/OR
cells,
Silencing
partially
reversed
resistance.
Additionally,
acted
as
competing
endogenous
(ceRNA)
by
targeting
miR-30a-3p,
Insulin-like
Growth
Factor
1
Receptor
(IGF1R)
identified
functional
gene
for
miR-30a-3p
Furthermore,
confirmed
axis
plays
role
activating
PI3K/AKT/mTOR
signaling
pathway
NSCLC
with
Conclusions
Therefore,
first
time
we
exerts
an
oncogenic
sponging
active
IGF1R/PI3K/AKT/mTOR
pathway.
may
serve
novel
diagnostic
biomarker
therapeutic
target
patients
Graphical
Exosomes
can
regulate
the
malignant
progression
of
tumors
by
carrying
a
variety
genetic
information
and
transmitting
it
to
target
cells.
Recent
studies
indicate
that
exosomal
circular
RNAs
(circRNAs)
multiple
biological
processes
in
carcinogenesis,
such
as
tumor
growth,
metastasis,
epithelial-mesenchymal
transition,
drug
resistance,
autophagy,
metabolism,
angiogenesis,
immune
escape.
In
microenvironment
(TME),
circRNAs
be
transferred
among
cells,
endothelial
cancer-associated
fibroblasts,
microbiota,
affecting
initiation
progression.
Due
high
stability
widespread
presence
circRNAs,
they
hold
promise
biomarkers
for
diagnosis
prognosis
prediction
blood
urine.
addition,
designing
nanoparticles
targeting
utilizing
derived
from
cells
or
stem
provide
new
strategies
cancer
therapy.
this
review,
we
examined
crucial
role
regulating
tumor-related
signaling
pathways
summarized
transmission
between
various
types
their
impact
on
TME.
Finally,
our
review
highlights
potential
diagnostic
prognostic
biomarkers,
well
suggesting
clinical
International Journal of Surgery,
Год журнала:
2025,
Номер
111(3), С. 2590 - 2602
Опубликована: Янв. 7, 2025
The
immune
response
is
modulated
by
a
diverse
array
of
signals
within
the
tissue
microenvironment,
encompassing
biochemical
factors,
mechanical
forces,
and
pressures
from
adjacent
tissues.
Furthermore,
extracellular
matrix
its
constituents
significantly
influence
function
cells.
In
case
carcinogenesis,
changes
in
biophysical
properties
tissues
can
impact
received
cells,
these
c1an
be
translated
into
through
mechano-transduction
pathways.
These
pathways
have
profound
on
cellular
functions,
influencing
processes
such
as
cell
activation,
metabolism,
proliferation,
migration,
etc.
Tissue
mechanics
may
undergo
temporal
during
process
offering
potential
for
novel
dynamic
levels
regulation.
Here,
we
review
advances
mechanoimmunology
malignancy
studies,
focusing
how
mechanosignals
modulate
behaviors
cells
at
level,
thereby
triggering
an
that
ultimately
influences
development
progression
malignant
tumors.
Additionally,
also
focused
mechano-immunoengineering
systems,
with
help
which
could
to
further
understand
tumor
or
alterations
microenvironment
provide
new
research
directions
overcoming
immunotherapeutic
resistance
Frontiers in Genetics,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 4, 2025
Introduction
Endoscopic
tissue
biopsy
combined
with
histopathology
is
the
gold
standard
for
diagnosis
of
colorectal
cancer
(CRC);
however,
invasive
nature
this
procedure
hinders
its
acceptance
by
patients.
Therefore,
there
exists
a
critical
need
to
identify
novel
markers
facilitating
early
CRC
detection
and
prognosis.
Circular
RNAs
(circRNAs)
hold
promise
as
clinical
diagnostic
markers.
This
study
aimed
investigate
impact
circDOCK1
on
metastasis
prognosis
well
underlying
molecular
mechanisms.
Methods
We
explored
circRNA
expression
profiles
in
four
pairs
tissues
adjacent
non-carcinoma
via
microarray
analysis.
After
Gene
Ontology
(GO),
Kyoto
Encyclopedia
Genes
Genomes
(KEGG),
circRNA–miRNA
network
analyses,
was
chosen
further
investigation.
evaluated
relevance
80
controls,
correlating
characteristics.
Follow-up
data
from
patient
telephone
interviews
were
analyzed
survival
outcomes.
Transfection
efficiency
confirmed
qRT-PCR
HCT116
SW480
colon
cells,
effects
cell
proliferation,
migration,
invasion
assessed.
Results
Microarray
revealed
149
significantly
differentially
expressed
circRNAs,
including
71
upregulated
78
downregulated
tissues.
CircDOCK1
exhibited
elevated
patients
emerged
an
independent
prognostic
factor.
Kaplan–Meier
curve
analysis
suggested
that
unfavorable
factor
CRC.
In
vivo
experiments
overexpression
enhanced
consistent
results
upon
downregulation.
Conclusion
These
indicate
may
play
role
promoting
suggesting
potential
biomarker.
Journal of International Medical Research,
Год журнала:
2025,
Номер
53(2)
Опубликована: Фев. 1, 2025
Early
diagnosis
and
timely
management
are
critical
for
determining
disease
outcomes
prognoses.
To
date,
certain
methods
developing
disease-specific
biomarkers
have
been
reported;
however,
strategies
musculoskeletal
biomarker
development
rarely
studied.
Recent
studies
highlighted
the
potential
application
of
extracellular
vesicles
(EVs)
as
biomarkers.
EVs
encapsulate
proteins,
lipids,
messenger
RNAs,
microRNAs
derived
from
their
cellular
origin;
these
constituents
remain
stable
within
can
traverse
blood–brain
barrier.
Because
distinctive
characteristics,
actively
investigated
diagnostic
tools
various
conditions,
including
cancer,
inflammatory
diseases,
disorders.
Although
many
advantages
development,
they
not
yet
fully
researched
in
context
pathologies.
The
current
review
aimed
to
highlight
biomarkers,
summarize
processes
EV
discuss
limitations
future
perspectives
iScience,
Год журнала:
2025,
Номер
28(4), С. 112183 - 112183
Опубликована: Март 12, 2025
Renal
cell
carcinoma
(RCC),
particularly
clear
RCC
(ccRCC),
has
seen
rising
incidence
and
mortality
rates.
Early
detection
remains
difficult
due
to
nonspecific
symptoms,
often
leading
diagnoses
at
advanced
stages
unfavorable
prognoses.
Current
treatments,
including
surgery,
targeted
therapies,
immunotherapies,
face
limitations
such
as
adverse
reactions,
drug
resistance,
immune
evasion.
This
review
discusses
the
role
of
circular
RNAs
(circRNAs)
their
involvement
in
circRNA/miRNA/mRNA
axis
during
progression.
As
competitive
endogenous
(ceRNAs),
circRNAs
regulate
gene
expression
cellular
processes,
proliferation,
metastasis,
apoptosis,
resistance.
Furthermore,
we
explore
impact
on
tumor
metabolic
reprogramming
influence
microenvironment.
We
also
summarize
clinical
prospects,
challenges,
future
directions
this
field.
A
comprehensive
understanding
these
complex
interactions
may
provide
new
insights
into
diagnosis
treatment
RCC,
ultimately
overcoming
existing
challenges
improving
patient
outcomes.
