Investigation of the Effects of Salinity Exposure on Immune Defense, Morphology, and Gene Expression in the Gills of Macrobrachium nipponense DOI Creative Commons
Shubo Jin, Rong Zhou, Hongtuo Fu

и другие.

Antioxidants, Год журнала: 2025, Номер 14(6), С. 655 - 655

Опубликована: Май 29, 2025

Macrobrachium nipponense is an important economic freshwater species in China. Previous research has found that M. can reproduce under salinity conditions of 10 parts per thousand (ppt) and exhibits a strong ability to adapt changes the aquatic environment. The aim present study was identify molecular mechanism terms saline acclimation by identifying immune response, morphology, gene expression gills ppt. findings revealed exposure dramatically stimulated activities MDA, Ca2+Mg2+-ATPase, CAT, reaching peak on Day 7 (p < 0.05), indicating these antioxidant enzymes play essential roles protecting body from damage caused treatment. In addition, we no obvious morphological gills, well water environments with such salinity. Transcriptome profiling analysis identified 168, 434, 944 differentially expressed genes (DEGs) when comparing S0 vs. S1, S1 S4, S4 S7, respectively. Furthermore, lysosome, apoptosis, amino sugar, nucleotide sugar metabolism; cGMP-PKG signaling pathway; pancreatic secretion; calcium pathway represented main enriched metabolic pathways DEGs study. Lysosome, metabolism are immune-related pathways, while secretion energy-metabolism-related pathway, suggesting response energy regulation this species. results quantitative real-time PCR analyses were consistent those RNA-Seq, accuracy This provides valuable evidence for high-salinity environments, thus potential be used aquaculture programs alkaline regions.

Язык: Английский

Investigation of the Effects of Salinity Exposure on Immune Defense, Morphology, and Gene Expression in the Gills of Macrobrachium nipponense DOI Creative Commons
Shubo Jin, Rong Zhou, Hongtuo Fu

и другие.

Antioxidants, Год журнала: 2025, Номер 14(6), С. 655 - 655

Опубликована: Май 29, 2025

Macrobrachium nipponense is an important economic freshwater species in China. Previous research has found that M. can reproduce under salinity conditions of 10 parts per thousand (ppt) and exhibits a strong ability to adapt changes the aquatic environment. The aim present study was identify molecular mechanism terms saline acclimation by identifying immune response, morphology, gene expression gills ppt. findings revealed exposure dramatically stimulated activities MDA, Ca2+Mg2+-ATPase, CAT, reaching peak on Day 7 (p < 0.05), indicating these antioxidant enzymes play essential roles protecting body from damage caused treatment. In addition, we no obvious morphological gills, well water environments with such salinity. Transcriptome profiling analysis identified 168, 434, 944 differentially expressed genes (DEGs) when comparing S0 vs. S1, S1 S4, S4 S7, respectively. Furthermore, lysosome, apoptosis, amino sugar, nucleotide sugar metabolism; cGMP-PKG signaling pathway; pancreatic secretion; calcium pathway represented main enriched metabolic pathways DEGs study. Lysosome, metabolism are immune-related pathways, while secretion energy-metabolism-related pathway, suggesting response energy regulation this species. results quantitative real-time PCR analyses were consistent those RNA-Seq, accuracy This provides valuable evidence for high-salinity environments, thus potential be used aquaculture programs alkaline regions.

Язык: Английский

Процитировано

0