Integrated Analysis of DNA Methylome and Transcriptome Reveals Regulatory Mechanism in the Longissimus Dorsi of Duroc Pigs DOI Creative Commons
Shiyin Li, Yingying Gao,

Lixia Ma

и другие.

Cells, Год журнала: 2025, Номер 14(11), С. 786 - 786

Опубликована: Май 27, 2025

DNA methylation plays a pivotal role in the epigenetic regulation of gene expression and holds promise for enhancing livestock meat production. In this study, we analyzed methylome transcriptome longissimus dorsi muscle (LDM) Duroc pigs with varying growth rates. Our results reveal that suppressed key development markers (MYOD, MYOG, MHC1) proliferation (PI67, PCNA), as well protein phosphorylation PI3K AKT (p < 0.05). Dual-luciferase reporter assays EMSA showed SP1 overexpression enhanced luciferase activity wild-type LPAR1 promoter, an effect amplified by demethylating agent 5-AZA The further demonstrates relationship between promoter region. Overexpression upregulated at both mRNA levels Knockdown reduced marker delayed myotube formation, while silencing disrupted LPAR1, MEF2C, MHC1 0.05), demethylation induced partially reversed these effects. These findings suggest methylation/SP1/LPAR1 axis is critical skeletal development, underscoring regulatory formation.

Язык: Английский

Integrative transcriptomic, proteomic and metabolomic analyses yields insights into muscle fiber type in cattle DOI
Xiaofan Tan, Ruixue Zhao, Jing Chen

и другие.

Food Chemistry, Год журнала: 2024, Номер 468, С. 142479 - 142479

Опубликована: Дек. 13, 2024

Язык: Английский

Процитировано

4
Supplementation of Enzymatic Hydrolysate in Low-Fishmeal and Low-Crop Diet Improves Growth, Antioxidant Capacity, and Immunity of Juvenile Sea Cucumber Apostichopus japonicus (Selenka) DOI Creative Commons
Q.-J. Li,

Z. Y. Liu,

Gang Yang

и другие.

Fishes, Год журнала: 2025, Номер 10(2), С. 42 - 42

Опубликована: Янв. 24, 2025

As the global demand for aquafeed ingredients continues to rise, sourcing sustainable alternatives is crucial aquaculture industries. This study aimed explore potential of enzymatic hydrolysate as a substitute traditional fishmeal and soybean meal in diets juvenile sea cucumbers (Apostichopus japonicus). Three isonitrogenous (15% crude protein) isolipidic (2.4% lipid) were formulated: control diet containing 10% 5% two experimental low-fishmeal (8%) low-soybean (0%) diets, supplemented with either 8% enzymatically hydrolyzed fish stickwater (EFS) or chicken pulp (ECP), designated Control, EFS, ECP, respectively. Juvenile (initial body weight, 0.25 ± 0.01 g) fed these 84 days evaluate effects ECP EFS on their growth performance, antioxidant capacity, inflammatory responses. The results revealed significantly higher final weights specific rates both groups than (p < 0.05). proximate chemical compositions cucumber less affected by > Compared group, elevated levels digestive enzymes, antioxidants, lysozyme, together lower malondialdehyde levels, recorded appeared exhibit greater potency enhancing performance capacity. Similar trends observed mRNA expression SOD, CAT, inflammation-related genes across groups. In nutshell, could effectively promote health A. japonicus, showing superior effect over EFS. These findings suggest that demonstrates viable alternative aquaculture. Further investigations are warranted reveal its underlying mechanism.

Язык: Английский

Процитировано

0
Genome-Wide Association Integrating a Transcriptomic Meta-Analysis Suggests That Genes Related to Fat Deposition and Muscle Development Are Closely Associated with Growth in Huaxi Cattle DOI Creative Commons
Chengli Liu, Tao Ren, Pengcheng Ruan

и другие.

Veterinary Sciences, Год журнала: 2025, Номер 12(2), С. 109 - 109

Опубликована: Фев. 2, 2025

Growth traits are among the most important economic phenotypes targeted in genetic improvement of beef cattle. To understand basis growth Huaxi cattle, we performed a genome-wide association study (GWAS) on body weight, eye muscle area, and back fat thickness across five developmental stages population 202 Additionally, publicly available RNA-seq data from longissimus dorsi both young adult cattle were analyzed to identify key genes markers associated with In total, 7.19 million high-quality variant loci (SNPs INDELs) identified all samples. GWAS, three multilocus models (FarmCPU, MLMM, BLINK) outperformed conventional single-locus (CMLM, GLM, MLM). Consequently, GWAS analysis was conducted using models, which 99 significantly annotated total 83 candidate (CDGs). 23 CDGs overlapped differentially expressed public datasets between Furthermore, gene functional enrichment (KEGG GO) analyses revealed that over 30% pathways GO terms development deposition, crucial factors for production. Specifically, included MGLL, SGMS1, SNX29 AKAP6, implicated lipid metabolism, adipogenesis, growth. summary, this provides new insights into mechanisms underlying presents promising future breeding improvements.

Язык: Английский

Процитировано

0
Integrative Analysis of ATAC-Seq and RNA-Seq Identifies Key Genes Affecting Muscle Development in Ningxiang Pigs DOI Open Access
Wenhua Tan, Chenxi Liu,

Juan Liu

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2634 - 2634

Опубликована: Март 14, 2025

Meat production traits in pigs are critical economic characteristics, primarily influenced by the formation and development of skeletal muscle. Skeletal muscle is regulated a complex transcriptional network, which partly relies on chromatin accessibility for initiation. Ningxiang pigs, renowned Chinese indigenous breed, highly valued their tender meat. However, studies focusing particularly from perspective accessibility, have not yet been reported. Based this, present study selected several key time points to perform Transposase-Accessible Chromatin Sequencing (ATAC-seq) RNA sequencing (RNA-seq). This was carried out identify open regions genes during different growth stages, could influence pigs. We collected longissimus dorsi samples at postnatal days 14 (D14), 28 (D28), 85 (D85), 165 (D165), 250 (D250). For each age, three individuals were ATAC-seq RNA-seq. After initial differential analysis among ages, we identified 6412 differentially accessible peaks 1464 expressed genes. To clarify candidate transcription factors affecting motif revealed potential cis-regulatory elements with binding sites factors, including Fosl2 JunB. Correlation 56 overlapping significant positive correlation (r = 0.73, p 1 × 10−14) between gene expression accessibility. Key such as HOXA10, closely related development, specifically examined. These results enhance our understanding genetic epigenetic regulatory mechanisms porcine providing robust foundation future molecular studies.

Язык: Английский

Процитировано

0
Integrated Analysis of DNA Methylome and Transcriptome Reveals Regulatory Mechanism in the Longissimus Dorsi of Duroc Pigs DOI Creative Commons
Shiyin Li, Yingying Gao,

Lixia Ma

и другие.

Cells, Год журнала: 2025, Номер 14(11), С. 786 - 786

Опубликована: Май 27, 2025

DNA methylation plays a pivotal role in the epigenetic regulation of gene expression and holds promise for enhancing livestock meat production. In this study, we analyzed methylome transcriptome longissimus dorsi muscle (LDM) Duroc pigs with varying growth rates. Our results reveal that suppressed key development markers (MYOD, MYOG, MHC1) proliferation (PI67, PCNA), as well protein phosphorylation PI3K AKT (p < 0.05). Dual-luciferase reporter assays EMSA showed SP1 overexpression enhanced luciferase activity wild-type LPAR1 promoter, an effect amplified by demethylating agent 5-AZA The further demonstrates relationship between promoter region. Overexpression upregulated at both mRNA levels Knockdown reduced marker delayed myotube formation, while silencing disrupted LPAR1, MEF2C, MHC1 0.05), demethylation induced partially reversed these effects. These findings suggest methylation/SP1/LPAR1 axis is critical skeletal development, underscoring regulatory formation.

Язык: Английский

Процитировано

0