Journal of Fish Biology, Год журнала: 2025, Номер unknown
Опубликована: Май 12, 2025
Abstract As a member of the bone morphogenetic protein (BMP) family, Bmp4 plays crucial role in formation, early developmental stage and regulation gonadal development. This study aims to investigate expression patterns potential functions Pampus argenteus during its reproductive cycle. A systematic evolutionary analysis was performed, quantified localized using real‐time polymerase chain reaction (PCR), Western blotting immunohistochemistry. Additionally, effects various hormones on hormone‐related genes were analysed vitro cultured testes, ovaries oocytes. The results revealed that is evolutionarily conserved broadly expressed across tissues. Notably, significantly increased at 4 13 days post‐hatching (dph), with no significant differences observed other stages. identified specific target band Stage III ovaries, immunohistochemistry oogonia, oocytes spermatogonia. In hormonal treatments demonstrated oestradiol (E2) upregulated bmp4 both testes levels being notably higher ovaries. immature vitellogenic vitro, sex hormone did not affect , lhr fshr lhβ . However, under LH + hCG treatment (50 ng/mL 5 IU/mL), fshβ upregulated. summary, our findings reveal serves as pivotal factor development gonads germ cells P. within ovary likely regulated by E2. During early‐stage ovary, E2 may jointly promote differentiation cells. offers valuable theoretical foundation for addressing challenges breeding process.
Язык: Английский