Cannabidiol-loaded poly lactic-co-glycolic acid nanoparticles with improved bioavailability as a potential for osteoarthritis therapeutic DOI Creative Commons

Zhiqiang Jin,

Yanting Zhan, Li Zheng

и другие.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Ноя. 26, 2022

Abstract Cannabidiol (CBD) is a non-intoxicating cannabinoid from cannabis sativa that has demonstrated efficacious against inflammation, which can be considered as potential drug for arthritis treatment. However, the poor solubility and low bioavailability limit its clinical application. Here, we report an effective strategy to fabricate CBD-loaded poly lactic-co-glycolic acid nanoparticles (CBD-PLGA-NPs). The CBD-PLGA-NPs exhibited spherical morphology average diameter of 238 nm. CBD was sustained release CBD-PLGA-NPs, improved CBD. Primary chondrocytes rat pups were isolated, LPS used induce inflammation in vitro simulate osteoarthritis (OA). effectively protect damage cell viability. What’s more, according results CCK-8 assay, hematoxylin-eosin staining, safranin O immunofluorescence real-time polymerase chain reaction observed significantly suppressed LPS-induced primary chondrocyte expression inflammatory cytokines, including interleukin 1β (IL-1β), 6 (IL-6), tumor necrosis factor-α (TNF-α) matrix metalloproteinase 13 (MMP-13). Remarkably, also showed better therapeutic effects inhibiting degradation extracellular than equivalent solution. In general, fabrication good protection promising system Significance study order improve CBD, described development simple efficient (CBD-PLGA-NPs) treating damaged. fabricated could enhance chondroprotective by factors, increasing cellularity, improving structural changes, regarded treat OA.

Язык: Английский

Molecular and Cellular Mechanisms of Action of Cannabidiol DOI Creative Commons
Nadia Martínez Naya,

Jazmin Kelly,

Giuliana Corna

и другие.

Molecules, Год журнала: 2023, Номер 28(16), С. 5980 - 5980

Опубликована: Авг. 9, 2023

Cannabidiol (CBD) is the primary non-psychoactive chemical from Cannabis Sativa, a plant used for centuries both recreational and medicinal purposes. CBD lacks psychotropic effects of Δ9-tetrahydrocannabinol (Δ9-THC) has shown great therapeutic potential. exerts wide spectrum at molecular, cellular, organ level, affecting inflammation, oxidative damage, cell survival, pain, vasodilation, excitability, among others, modifying many physiological pathophysiological processes. There evidence that may be effective in treating several human disorders, like anxiety, chronic psychiatric pathologies, cardiovascular diseases, even cancer. Multiple cellular pre-clinical studies using animal models disease trials have an overall safe profile. In this review article, we summarize pharmacokinetics data, putative mechanisms action CBD, reported to give comprehensive list findings major attributed compound.

Язык: Английский

Процитировано

62
Anti-inflammatory effect of walnut-derived peptide via the activation of Nrf2/Keap1 pathway against oxidative stress DOI Creative Commons
Yuan Qi, Dan Wu, Li Fang

и другие.

Journal of Functional Foods, Год журнала: 2023, Номер 110, С. 105839 - 105839

Опубликована: Окт. 14, 2023

This study aimed to investigate the anti-inflammatory effect of walnut-derived peptide LPLLR (LP-5) in oxidative stress-induced Caco-2 cells and model mice. The results showed that LP-5 significantly increased activities CAT SOD, while decreased levels MDA both vitro vivo. stress signaling was regulated by promoting Nrf2 nuclear transfer, up-regulating HO-1 expression, down-regulating Keap1 expression. As demonstrated molecular docking, could effectively bind Keap1. Additionally, inhibited activation NLRP3 inflammasome via expression NLRP3, ASC, caspase-1, reducing release inflammatory cytokines, including IL-18, IL-1β, TNF-α, thereby preventing cell apoptosis. Moreover, production IL-10 iNOS COX-2 cells. These data suggested ameliorated stress-mediated inflammation inhibiting activating Nrf2/Keap1 signaling.

Язык: Английский

Процитировано

14
Cannabidiol‐Loaded Poly Lactic‐Co‐Glycolic Acid Nanoparticles with Improved Bioavailability as a Potential for Osteoarthritis Therapeutic DOI

Zhiqiang Jin,

Yanting Zhan, Li Zheng

и другие.

ChemBioChem, Год журнала: 2023, Номер 24(9)

Опубликована: Фев. 16, 2023

Abstract Cannabidiol (CBD) is a non‐intoxicating cannabinoid from cannabis sativa that has demonstrated efficacious against inflammation, which can be considered as potential drug for arthritis treatment. However, the poor solubility and low bioavailability limit its clinical application. Here, we report an effective strategy to fabricate Cannabidiol‐loaded poly(lactic‐co‐glycolic acid) copolymer (CBD‐PLGA) nanoparticles (NPs), with spherical morphology average diameter of 238 nm. CBD was sustained release CBD‐PLGA‐NPs, improved CBD. The CBD‐PLGA‐NPs effectively protect damage LPS cell viability. We observed significantly suppressed LPS‐induced primary rat chondrocyte expression inflammatory cytokines, including interleukin 1β (IL‐1β), 6 (IL‐6), tumor necrosis factor‐α (TNF‐α) matrix metalloproteinase 13 (MMP‐13). Remarkably, also showed better therapeutic effects inhibiting degradation extracellular chondrocytes than equivalent solution. In general, fabrication good protection in vitro promising system osteoarthritis

Язык: Английский

Процитировано

13
Cannabinoids improve mitochondrial function in skeletal muscle of exhaustive exercise training rats by inhibiting mitophagy through the PINK1/PARKIN and BNIP3 pathways DOI
Juncheng Si, Lili Sun, Ying� Qin

и другие.

Chemico-Biological Interactions, Год журнала: 2024, Номер 389, С. 110855 - 110855

Опубликована: Янв. 4, 2024

Язык: Английский

Процитировано

5
Effects of Oral Cannabidiol on Health and Fitness in Healthy Adults: An 8-Week Randomized Trial DOI Open Access
Victoria Flores, Jacob N. Kisiolek,

Arjun Ramani

и другие.

Nutrients, Год журнала: 2023, Номер 15(12), С. 2664 - 2664

Опубликована: Июнь 7, 2023

There is a lack of research on the effects cannabidiol (CBD) health-related fitness, physical activity, cognitive health, psychological wellbeing, and concentrations C-reactive protein (CRP) in healthy individuals. CBD has potential anti-inflammatory neuroprotective effects.

Язык: Английский

Процитировано

11
Cannabidiol-loaded hydrogel microneedle patches inhibit TRIM14/TRAF3/ NF-κB axis for the treatment of psoriasis DOI
Mengyan Li,

D Li,

Yu Zhang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 304, С. 140825 - 140825

Опубликована: Фев. 9, 2025

Язык: Английский

Процитировано

0
Development of Edible Carbohydrate–Protein Sports Gels to Optimize the Muscle Glycogen Re-Synthesis DOI Creative Commons

Vishal Verma,

V Biesold-McGee Gill,

Avinash Kumar

и другие.

Gels, Год журнала: 2025, Номер 11(5), С. 341 - 341

Опубликована: Май 2, 2025

This study was aimed at providing athletes a solution to replenish the muscle glycogen re-synthesis an optimal rate with hemp seeds as natural protein source and Bengal gram dal its use for preparation of gel. The gel contains richest energy, it is effective way provide energy nutrients athletes. prepared in three variations different seed concentrations. We then analyzed pH macronutrient composition. sensory characteristics were seven parameters, including appearance, taste, color, texture, aroma, consistency, acceptability, using hedonic scale on 25 panelists. A analysis showed that sample received overall acceptability score 7.16 ± 0.99 from panel. shelf life observed recommended temperature 4 degrees Celsius, which 12 days. best formulation B 38 g seeds, better lower average value (6.68 1.44, 6.56 1.29, 7.6 1.16, 7 1.26, 5.822 0.0183, respectively). Sample contained 30.8 protein, 16.09 carbohydrates, 8.4 fat, 263.16 kcal per 100 g. resulting ratio carbohydrates high-protein post-workout meal. Hence, can be considered supplement.

Язык: Английский

Процитировано

0
Suk-SaiYasna Remedy, a Traditional Thai Medicine, Mitigates Stress-Induced Cognitive Impairment via Keap1-Nrf2 Pathway DOI Open Access

Wuttipong Masraksa,

Supawadee Daodee, Orawan Monthakantirat

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(11), С. 5388 - 5388

Опубликована: Июнь 4, 2025

Suk-SaiYasna (SSY) is a well-documented traditional Thai herbal formula in the Royal Scripture of King Narai’s Traditional Medicine. SSY contains Cannabis sativa leaves as key ingredient and has traditionally been used to promote sleep, alleviate stress-related symptoms, stimulate appetite. This study aimed investigate neuroprotective effects mouse model unpredictable chronic mild stress (UCMS)-induced cognitive impairment explore underlying mechanisms, particularly antioxidant enzyme pathways. Behavioral tests, including Y-maze test, novel object recognition Morris water maze demonstrated that UCMS-exposed mice exhibited compared non-stress mice. However, treatment significantly improved learning memory performance Mechanistic studies revealed reduced lipid peroxidation hippocampus frontal cortex, brain regions affected by stress. Furthermore, UCMS activity enzymes superoxide dismutase (SOD) catalase (CAT), whereas restored their activity, indicating antioxidative vivo. Gene expression analysis further regulates oxidative via Nrf2/Keap1 signaling pathway. In vitro using 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay confirmed radical scavenging activities its components, demonstrating significant potential. Phytochemical identified delta-9-tetrahydrocannabinol, delta-9-tetrahydrocannabinolic A, cannabinoids bioactive compounds SSY, along with potent antioxidants such gallic acid, myricetin, myristicin, piperine, costunolide, gingerol. These findings suggest mitigates UCMS-induced function through properties multiple pathways, activities, modulating Nrf2-Keap1 pathway, inducing HO-1, NQO1 mRNAs, other enzymes. work bridges medicine modern neuropharmacology.

Язык: Английский

Процитировано

0
Therapeutic potentials of cannabidiol: Focus on the Nrf2 signaling pathway DOI Open Access
Marjan Talebi,

Mohammad Mehdi Sadoughi,

Seyed Abdulmajid Ayatollahi

и другие.

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 168, С. 115805 - 115805

Опубликована: Окт. 31, 2023

Cannabidiol (CBD), a cannabinoid that does not create psychoactive activities, has been identified as having multitude of therapeutic benefits. This study delves into the chemical properties, pharmacokinetics, safety and toxicity, pharmacological effects, most importantly, association between potential CBD nuclear factor erythroid 2-related 2 (Nrf2) signaling pathway. The relationship Nrf2 is closely linked to certain proteins are associated with cardiovascular dysfunctions, cancers, neurodegenerative conditions. Specifically, connected initiation progression diverse health issues, including nephrotoxicity, bladder-related diseases, oral mucositis, obesity, myocardial injury angiogenesis, skin-related inflammations, psychotic disorders, neuropathic pain, Huntington's disease, Alzheimer's Parkinson's neuroinflammation, Amyotrophic Lateral Sclerosis, Multiple Sclerosis. zone great interest in medical field, it significantly impact treatment prevention wide-ranging Additional investigation necessary entirely apprehend mechanisms underlying this crucial interplay develop effective interventions.

Язык: Английский

Процитировано

8
Integrated Metabolomics and Network Pharmacology Investigation of Cardioprotective Effects of Myricetin after 1-Week High-Intensity Exercise DOI Open Access
Tianyou Li, Le Wang, Luting Wu

и другие.

Nutrients, Год журнала: 2023, Номер 15(6), С. 1336 - 1336

Опубликована: Март 9, 2023

Cardiovascular adverse effects caused by high-intensity exercise (HIE) have become a public health problem of widespread concern. The therapeutic effect and metabolic regulation mechanism myricetin, phytochemical with potential effects, rarely been studied. In this study, we established mice models different doses myricetin intervention 1 week HIE after intervention. Cardiac function tests, serology, pathological examinations were used to evaluate the protective on myocardium. possible targets obtained using an integrated analysis metabolomics network pharmacology verified molecular docking RT-qPCR experiments. Different concentrations improved cardiac function, significantly reduced levels myocardial injury markers, alleviated ultrastructural damage, area ischemia/hypoxia, increased content CX43. We regulated combined validated them RT-qPCR. conclusion, our findings suggest that exerts anti-cardiac through downregulation PTGS2 MAOB upregulation MAP2K1 EGFR while regulating complicated network.

Язык: Английский

Процитировано

6