Post-translational
modifications
(PTMs)
of
α-synuclein
(α-syn)
such
as
acetylation
and
phosphorylation
play
important
yet
distinct
roles
in
regulating
α-syn
conformation,
membrane
binding,
amyloid
aggregation.
However,
how
PTMs
regulate
function
presynaptic
terminals
remains
unclear.
Previously,
we
reported
that
clusters
synaptic
vesicles
(SV)
1
,
neutral
phospholipid
lysophosphatidylcholine
(LPC)
can
mediate
this
clustering
2
.
Here,
based
on
our
previous
findings,
further
demonstrate
N-terminal
acetylation,
which
occurs
under
physiological
conditions
is
irreversible
mammalian
cells,
significantly
enhances
the
functional
activity
SVs.
Mechanistic
studies
reveal
enhancement
caused
by
N-acetylation-promoted
insertion
α-syn’s
N-terminus
increased
intermolecular
interactions
LPC-containing
membrane.
Our
work
demonstrates
N-acetylation
fine-tunes
α-syn–LPC
interaction
for
mediating
SV
clustering.
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Авг. 5, 2024
Protein
post-translational
modification
(PTM)
is
a
covalent
process
that
occurs
in
proteins
during
or
after
translation
through
the
addition
removal
of
one
more
functional
groups,
and
has
profound
effect
on
protein
function.
Glycosylation
most
common
PTMs,
which
polysaccharides
are
transferred
to
specific
amino
acid
residues
by
glycosyltransferases.
A
growing
body
evidence
suggests
glycosylation
essential
for
unfolding
various
activities
organisms,
such
as
playing
key
role
regulation
function,
cell
adhesion
immune
escape.
Aberrant
also
closely
associated
with
development
diseases.
Abnormal
patterns
linked
emergence
health
conditions,
including
cancer,
inflammation,
autoimmune
disorders,
several
other
However,
underlying
composition
structure
glycosylated
have
not
been
determined.
It
imperative
fully
understand
internal
differential
expression
glycosylation,
incorporate
advanced
detection
technologies
keep
knowledge
advancing.
Investigations
clinical
applications
focused
sensitive
promising
biomarkers,
effective
small
molecule
targeted
drugs
emerging
vaccines.
These
studies
provide
new
area
novel
therapeutic
strategies
based
glycosylation.
Chemical Society Reviews,
Год журнала:
2024,
Номер
53(10), С. 4976 - 5013
Опубликована: Янв. 1, 2024
Protein
misfolding
and
amyloid
aggregation,
linked
to
neurodegenerative
diseases,
can
result
from
liquid–liquid
phase
separation
(LLPS)
a
subsequent
liquid-to-solid
transition.
This
represents
LLPS
as
generic
mechanism
in
nucleation.
Chemical Reviews,
Год журнала:
2024,
Номер
124(5), С. 2805 - 2838
Опубликована: Фев. 19, 2024
Post-translational
modifications
(PTMs)
endow
proteins
with
new
properties
to
respond
environmental
changes
or
growth
needs.
With
the
development
of
advanced
proteomics
techniques,
hundreds
distinct
types
PTMs
have
been
observed
in
a
wide
range
from
bacteria,
archaea,
and
eukarya.
To
identify
roles
these
PTMs,
scientists
applied
various
approaches.
However,
high
dynamics,
low
stoichiometry,
crosstalk
between
make
it
almost
impossible
obtain
homogeneously
modified
for
characterization
site-specific
effect
individual
PTM
on
target
proteins.
solve
this
problem,
genetic
code
expansion
(GCE)
strategy
has
introduced
into
field
studies.
Instead
modifying
after
translation,
GCE
incorporates
amino
acids
during
thus
generating
site-specifically
at
positions.
In
review,
we
summarize
systems
orthogonal
translation
installation
PTMs.
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Фев. 21, 2024
Abstract
Phase
separation
is
a
cellular
phenomenon
where
macromolecules
aggregate
or
segregate,
giving
rise
to
biomolecular
condensates
resembling
"droplets"
and
forming
distinct,
membrane-free
compartments.
This
process
pervasive
in
biological
cells,
contributing
various
essential
functions.
However,
when
phase
goes
awry,
leading
abnormal
molecular
aggregation,
it
can
become
driving
factor
the
development
of
diseases,
including
tumor.
Recent
investigations
have
unveiled
intricate
connection
between
dysregulated
tumor
pathogenesis,
highlighting
its
potential
as
novel
therapeutic
target.
article
provides
an
overview
recent
research,
with
particular
emphasis
on
role
tumor,
implications,
outlines
avenues
for
further
exploration
this
intriguing
field.
Cells,
Год журнала:
2024,
Номер
13(5), С. 394 - 394
Опубликована: Фев. 25, 2024
Glycoproteomics
has
accelerated
in
recent
decades
owing
to
numerous
innovations
the
analytical
workflow.
In
particular,
new
mass
spectrometry
strategies
have
contributed
inroads
O-glycoproteomics,
a
field
that
lags
behind
N-glycoproteomics
due
several
unique
challenges
associated
with
complexity
of
O-glycosylation.
This
review
will
focus
on
progress
sample
preparation,
enrichment
strategies,
and
MS/MS
techniques
for
identification
characterization
O-glycoproteins.
